Influence of Dried Egg Yolk with or without Taurine in Extender on Sperm Characteristic of Cryopreserved Buffalo Semen

Abstract

The objective of this study is to determine the influence of dried egg yolk (DEY) powder on freezability of semen buffalo as an alternative of fresh egg yolk (FEY) with or without taurine. Semen was collected from five buffalo bulls with total motility (≥70% mass motility), pooled, extended (1: 10) with different tris extenders containing 20% FEY versus 5, 10, 15 and 20% DEY (1st experiment). Tris-EY extender contained 15% DEY was supplemented with taurine (0, 25, 50, 75 and 100 mM) in 2nd experiment. Results showed that increases in progressive motility at concentration 10 , 15% than 20% in DEY and FEY respectively, after dilution, equilibration, and thawing, Also, acrosome integrity percentage in post-equilibrated and thawed semen was improved (P <0.05) by 5, 10, and 15% DEY as compared with FEY, being the highest with 15% DEY. Adding 25 or 50-Mm taurine improved progressive motility after dilution, and acrosome integrity after equilibration, and membrane integrity percentage after all stages of cryopreservation. the powdered egg yolk at concentration of 10 or 15% may be used, as an alternative of fresh egg yolk in the diluents of buffalo sperm freezing. Addition of taurine at a level of 25 and 50 mM has possible protective effects as an antioxidant to improve semen quality of buffalo semen extended with 15% dried egg yolk.

Effect of Guava Filtrate Supplementation in Tris and Citrate-Based Extenders on Spermatozoa Quality of Brangus Bull after Sex-rest

“…Extenders based on commercial synthetic and an animal materials tend to be at risk of contamination with bacteria or mycoplasma, and easily damage during storage and harm spermatozoa (Delgado et al, 2018). In addition, egg yolks contain substances that interfere with biochemical and metabolic tests, can inhibit respiration, and potentially reduce spermatozoa motility (Pillet et al, 2011;Sakr et al, 2021). Tris-egg yolk proteins could also be phosphorylated by the spermatozoa during the incubation and attach to the sperm membranes so that adhering to the sperm membrane and limiting their movements (Bertuzzi et al, 2020).…”

“…Reducing a half proportion of egg yolk and replaced with GF makes the extender in T4 more preservative than other treatments. Egg yolks contain substances that interfere with biochemical and metabolic tests, can inhibit respiration, and potentially reduce spermatozoa motility (Pillet et al, 2011;Sakr et al, 2021).…”

“…The low density lipoprotein (LDL) can reduce the loss of phospholipids, grab toxins from plasma seminal proteins and stabilize the membrane (Manjunath et al, 2012;Akhter et al, 2010). However, egg yolks contain substances that interfere with biochemical and metabolic tests, can inhibit respiration, and potentially reduce spermatozoa motility (Pillet et al 2011;Sakr et al, 2021).…”