Protective Effect of Dietary Curcumin and Capsaicin on LPS-Induced Inflammation in Mice

Abstract

Objective:The current study aimed to evaluate the anti-inflammatory potency of combined curcumin and capsaicin against lipopolysaccharide (LPS) induced organ damage in mice. Methods: Adult male albino mice were distributed into five experimental groups for treatment with olive oil, LPS, curcumin, capsaicin and their combination, respectively, for 7 days prior to LPS induced inflammation. At the end of the experiment, blood samples were collected and used for the analysis of serum non-specific enzymes including serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin (TB), urea, creatinine and sugar, while the organ homogenates were subjected for the evaluation of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutothione S transferase (GST), nitric oxide (NO); lipid peroxidation (LPO) and it was further confirmed by histopathological study of different organs. Results and Conclusion: Curcumin, capsaicin and their combination had shown significant restoration of non-specific serum enzymes, antioxidant enzymes and attenuated inflammatory cells infiltration thereby preventing tissue/organ damage in LPS-challenged mice. However, the protective effect was found to be more when the two compounds were fed in combination. This beneficial potency of combined spice treatment is may be due to the contribution of diversified active moieties of curcumin and capsaicin in combination compared to individual molecules.

The comparison between curcumin and propolis against sepsis-induced oxidative stress, inflammation, and apoptosis in kidney of adult male rat

“…The SOD and CAT found to be critical parameters in sepsis; elevation of SOD and CAT caused accumulation of H 2 O 2 in cells. Meanwhile, Vasanthkumar et al [23] reported similar increments in LPO and NO after sepsis induction in mice using LPS.…”

“…Curcumin markedly inhibited hemolysis and lipid peroxidation of erythrocytes induced by linoleate by functioning as a scavenger of NO and blocking its synthesizing enzyme [25]. Curcumin also markedly decreased LPO and NO in the kidney of sepsis-induced mice [23]. C66, a novel curcumin derivative, was reported to reduce TNF-α, IL-1β, COX-2, and NF-κB production, in high glucose-stimulated diabetic rats [43].…”

Spices and herbs as immune enhancers and anti-inflammatory agents: A review

“…The anti-inflammatory effects of capsaicin may be enhanced when used in combination with other spices. One study showed that pre-treatment of mice with a combination of capsaicin and curcumin (the main active ingredient in turmeric) resulted in better protection against LPS-induced inflammation than capsaicin alone (Vasanthkumar et al, 2018). This result highlights the potential beneficial impact of combining spices.…”