Protective effect of dexamethasone on fetal rat skin in experimental intrauterine ischaemia/reperfusion injury

Kaptanoğlu, Aslı Feride; Arca, Turkan; Sargon, Mustafa F.; Kılınç, Kamer

doi:10.1111/ced.12019

Cited by 4 publications

(4 citation statements)

References 28 publications

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“…The samples were dehydrated in gradient alcohol, washed with propylene oxide, embedded in epoxy resin, and sectioned into 60‐nm ultrathin slices. After sectioning, the samples were stained with uranyl acetate and lead citrate and examined by TEM (JEM 1230 EX; JEOl, Japan) as described by Kaptanoglu et al…”

Section: Methodsmentioning

confidence: 99%

“…After sectioning, the samples were stained with uranyl acetate and lead citrate and examined by TEM (JEM 1230 EX; JEOl, Japan) as described by Kaptanoglu et al 17 The membrane was washed with TBS-T for 1 hour and incubated with horseradish peroxidase-conjugated goat anti-rabbit antibody diluted 1:1000 in TBS-T for detection of bound antibodies by enhanced chemiluminescence. Mouse monoclonal GAPDH antibody was used as the loading control.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

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Role of the p38 mitogen‐activated protein kinase signaling pathway in estrogen‐mediated protection following flap ischemia‐reperfusion injury

Hou

2016

Cell Biochemistry & Function

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Ischemia-reperfusion (I/R) injury often occurs during skin flap transplantation and results in tissue damage. Although estrogen treatment significantly alleviates this I/R injury-induced damage, the detailed molecular mechanism is not clear. In this study, a superficial epigastric artery flap I/R injury model was created in adult Wistar rats. Severe necrosis was observed in skin tissue after I/R injury. Histological examination of skin tissue revealed that I/R injury damages skin structure and results in neutrophil infiltration. Inflammation-related parameters, including neutrophil count, tumor necrosis factor-α, and interleukin-10 levels, were increased due to I/R injury. These pathological phenomena were reduced by estradiol treatment. Further investigation found that I/R injury triggers the p38 mitogen-activated protein kinase (p38-MAPK) pathway. The expression levels of p38-MAPK and phosphorylated p38-MAPK were increased after I/R injury. Estradiol increased the expression level of MAPK phosphatase-2, a putative phosphatase of p38, and reduced the levels of p38-MAPK and phosphorylated p38-MAPK. These results suggest that estradiol can improve skin flap survival, possibly by inhibiting neutrophil infiltration and the expression of p38-MAPK. This study provides an explanation for how estrogen alleviates I/R injury-induced damage that occurs during skin flap transplantation. In a rat pathological model, I/R injury leads to skin necrosis, skin structure damage, neutrophil infiltration, and inflammatory cytokine secretion, which are probably downstream effects of activation of the p38-MAPK pathway. On the other hand, estradiol treatment triggers the expression of MAPK phosphatase-2, a putative phosphatase of p38-MAPK, and reduced all examined pathological phenomena. Therefore, estrogen may reduce the deleterious effect of I/R injury on skin flap transplantation through modulating the p38-MAPK pathway.

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Section: Methodsmentioning

confidence: 99%

Section: Transmission Electron Microscopymentioning

confidence: 99%

Role of the p38 mitogen‐activated protein kinase signaling pathway in estrogen‐mediated protection following flap ischemia‐reperfusion injury

Hou

2016

Cell Biochemistry & Function

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“…The specific definition of IUGR is a foetus whose estimated weight is below the 10 th percentile or beyond two standard deviations for its gestational age and a neonatal birth weight below 2,500 g after full-term pregnancy (1,2). Based on current epidemiological analysis and clinical evidence, the morbidity of IUGR is approximately 5% to 12% in the perinatal period, and IUGR likely accounts for 10%-15% of pregnancy complications (3)(4)(5). IUGR has adverse effects on early life.…”

Section: A Model Of Intrauterine Growth Restriction Induced By Bilatementioning

confidence: 99%

A Model of Intrauterine Growth Restriction Was Induced by Bilateral Uterine Vessel Clamping in Pregnant Rats

Diao¹,

Li²,

Qu³

et al. 2017

J Anesth Perioper Med

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Background: Intrauterine growth restriction (IUGR) is considered to be a major risk for cerebral palsy and disability after birth. However, IUGR lacks effective treatment strategies. We aimed to establish a rat model of bilateral uterine artery ischemia-reperfusion as a surgical model of IUGR. Methods: At the 17th day of pregnancy, six Sprague-Dawley (SD) rats were randomly divided into sham (NOR) and growth restriction (GR) groups. In the GR groups, four small artery clamps were used to occlude the uterine vessels near the lower and upper ends of the bilateral horns for 25 min; then, the clamps were removed, and the horns were placed back into the abdomen cavity. In the sham-operated animals, the uterine horns were exposed for 25 min without artery clamping. After labour and delivery, the death rates and weights during the first 8 days of newborn rats were recorded. A pathological examination was performed with hematoxylin-eosin staining (HE) staining and terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling staining (TUNEL) on 4-day-old and 8-day-old brain tissues of the newborn rats. Results: The mortality within the first 24 h of rats in the GR group were increased (66.7% vs 0.0% ), and their daily weights within the first 8 postnatal days were decreased. In addition, the karyopyknosis percentages of 4-day-old and 8-day-old rats were increased (4-day-old: 45% vs 11%; 8-day-old: 34.7% vs 5.3%), and the numbers of TUNEL-positive cells were increased (4-day-old: 110 vs 12.7; 8-day-old: 146.7 vs 4). Conclusions: This model of foetal IUGR produced a decrease in weight, an increase in the death rate within 24 h and an increase in apoptosis in newborn rats. The establishment of the model was successful.

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“…Also, full support is supplied by the other departments of the university such as in infectious diseases (bacteriology and micology lab), genetic (e.g: PCR) and pathology (immunohistochemistry and immunoflorescein dyes). In addition to daily clinical practice and teaching dermatology to the medical students, the dermatologists make research in experimental, clinical and public health areas, as well as conducting continuing medical education activities such as sessions of case discussions, and seminars [6][7][8][9][10]. Due to the lack of academic staff in number, the department does not accept residents yet.…”

Section: Dermatologists As Community Leadersmentioning

confidence: 99%

The history of dermatology, venereology and dermatopathology in different countries - North Cyprus

Kaptanoğlu¹,

Mullaaziz²

2016

Glob Dermatol

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Protective effect of dexamethasone on fetal rat skin in experimental intrauterine ischaemia/reperfusion injury

Abstract: Maternal treatment with dexamethasone may have a protective effect on fetal skin in cases of I/R injury.

Cited by 4 publications

References 28 publications

Role of the p38 mitogen‐activated protein kinase signaling pathway in estrogen‐mediated protection following flap ischemia‐reperfusion injury

Role of the p38 mitogen‐activated protein kinase signaling pathway in estrogen‐mediated protection following flap ischemia‐reperfusion injury

A Model of Intrauterine Growth Restriction Was Induced by Bilateral Uterine Vessel Clamping in Pregnant Rats

The history of dermatology, venereology and dermatopathology in different countries - North Cyprus

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