Protective effect of chelerythrine against ethanol-induced gastric ulcer in mice

Li, Weifeng; Hao, Dingjun; Fan, Ting; Huang, Huimin; Yao, Huan; Niu, Xiaofeng

doi:10.1016/j.cbi.2013.11.011

Cited by 111 publications

(82 citation statements)

References 41 publications

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“…NO is a key protective factor for the gastric mucosa, and can regulate the secretion of gastric acid, promote the synthesis between the gastric mucus and mucoprotein, maintain and strengthen the mucous barrier function and clear oxygen free radicals (26). Polyphenols in Dragon-pearl tea may increase PGE2 and NO levels in the serum, improve microcirculation in the gastric mucosa, clear oxygen free radicals and strengthen the mucous barrier function.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

Wang

Sun

2015

Experimental and Therapeutic Medicine

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Abstract. Dragon-pearl tea is a type of green tea commonly consumed in Southwest China. In the present study, the antioxidative and anti-gastric ulcer effects of Dragon-pearl tea crude polyphenols (DTCP) were determined in vitro and in vivo. Treatment with 25, 50 or 100 µg/ml DTCP resulted in notable antioxidant effects in vitro, which manifested as 2,2-diphenyl-1-picrylhydrazyl and OH radical-scavenging activity. Furthermore, using an in vivo mouse model, DTCP was shown to reduce the gastric ulcer area in the stomach, in which the 200 mg/kg DTCP dose exhibited the most marked effect, with a gastric ulcer index inhibitory rate of 72.63%. In addition, DTCP was demonstrated to improve stomach acidity conditions in vivo by increasing the pH and reducing the level of gastric juice, as compared with the reserpine-induced gastric ulcer control mice. Furthermore, DTCP altered the serum levels of a number of oxidation-related biomolecules, including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), lipid peroxidation (LPO) and catalase (CAT), to subsequently exert an anti-gastric ulcer effect. Treatment with 50, 100 and 200 mg/kg DTCP increased the SOD, GSH-Px and CAT levels and reduced the MDA and LPO levels in the mouse model of gastric ulcers. These serum level alterations resulted in the modified serum levels of prostaglandin E2 (PGE2) and nitric oxide (NO), which are associated with gastric mucosal protection. A reverse transcription-quantitative polymerase chain reaction (RT-PCR) assay is a molecular biology experiment which could determine the changes of mRNA in tissues. Using the RT-PCR assay, DTCP was observed to increase the mRNA expression levels of certain genes associated with gastric ulcers: Epidermal growth factor, epidermal growth factor receptor, vascular endothelial growth factor and vascular endothelial growth factor receptor 1, while reducing gastrin expression levels. Therefore, the results indicated that DTCP induced a marked preventative effect on reserpine-induced gastric ulcers in vivo, as a result of its antioxidative capacity.

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Section: Discussionmentioning

confidence: 99%

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

Wang

Sun

2015

Experimental and Therapeutic Medicine

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“…An immunohistochemical analysis showed that the expression of cyclooxygenase (COX)-2 was significantly suppressed in vivo in mice without affecting the inhibition of COX-1 (Li et al 2014b). The protective effect of chelerythrine on an ethanol-induced gastric ulcer in mice has been described recently (Li et al 2014a). It significantly reduced the gastric ulcer index, myeloperoxidase activities, and macroscopic and histological score in a dose-dependent manner.…”

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confidence: 90%

The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-α secretion and cyclooxygenase activity

et al. 2017

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Quaternary benzophenanthridine alkaloids are known to have a wide range of biological effects, including antimicrobial, antifungal, anti-inflammatory, and antitumour activities. However, only sanguinarine and chelerythrine have been studied intensively. The aim of this study was to evaluate the anti-inflammatory potential of the five minor quaternary benzophenanthridine alkaloids sanguilutine, sanguirubine, chelirubine, chelilutine, and macarpine in vitro and to compare them with more thoroughly studied sanguinarine and chelerythrine. Before making cellbased assays, the cytotoxicity of the alkaloids was evaluated. The anti-inflammatory potential of the chosen alkaloids was evaluated as for their ability to modulate the lipopolysaccharideinduced secretion of tumour necrosis factor α (TNF-α) in the macrophage-like cell line THP-1. The cyclooxygenase (COX)-1 and COX-2 inhibitory activities were also measured. The results indicate that the presence of a methylenedioxy ring attached at carbon (C)7-C8 is important for reducing the secretion of TNF-α. Interestingly, this effect did not show a simple dependence on concentration. The selected alkaloids showed little or no anti-COX activity. The results obtained from the present experiments may provide additional information useful in understanding the structure-to-activity relationship of the quaternary benzophenanthridine alkaloids. The antiinflammatory potential and the cytotoxic effect are driven by the presence of a methylenedioxy ring attached at C7-C8 and C2-C3, respectively.

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“…It is well-established that various free radicals, including DPPH, ABTS radicals and superoxide, and other reactive species, such as singlet oxygen, hypochlorous acid and peroxynitrite, contribute to the pathology of chronic inflammation (27,28). Previous studies have suggested that inflammatory tissue damage may be due to the liberation of reactive oxygen species from the phagocytes that invade inflammation sites (29,30).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and anti-inflammatory activities of ethyl acetate extract of Gynura formosana (Kitam) leaves

Guo

Pan

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Gynura formosana Kitam (family Compositae) has traditionally been used for the prevention of diabetes, cancer and inflammation in China. However, there are few reports of its anti-inflammatory effects. In the present study, after assessing the in vitro antioxidant activities of extracts from Gynura formosana Kitam leaves, the anti-inflammatory and antioxidant activities of ethyl acetate extract of Gynura formosana Kitam leaves (EAEG) were further investigated in rats using a cotton pellet-induced model of granuloma. EAEG significantly inhibited the formation of cotton pellet-induced granuloma in rats in a dose-dependent manner. Moreover, EAEG treatment significantly decreased the level of plasma C-reactive protein and suppressed the activities of plasma glutamate pyruvate transaminase and lactate dehydrogenase in model groups. Furthermore, EAEG increased the hepatic levels of anti-oxidative enzymes or antioxidants, including catalase, superoxide dismutase and reduced glutathione, and reduced the level of lipid peroxidation in the rat model of inflammation. In addition, EAEG decreased plasma levels of tumor necrosis factor-α and interleukin-1β in the rat model of inflammation. Therefore, our results indicated that EAEG had potent anti-inflammatory effects, which was achieved at least in part through activating antioxidant enzyme activities and suppressing the production of proinflammatory mediators by macrophages.

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Protective effect of chelerythrine against ethanol-induced gastric ulcer in mice

Cited by 111 publications

References 41 publications

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-α secretion and cyclooxygenase activity

Antioxidant and anti-inflammatory activities of ethyl acetate extract of Gynura formosana (Kitam) leaves

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