Protective effect of Aurelia aurita against free radicals and streptozotocin-induced diabetes

Talluri, Mallikarjuna Rao; Ketha, Alekhya; Battu, Ganga Rao; Tadi, Rajananda Swamy; Tatipamula, Vinay Bharadwaj

doi:10.3329/bjp.v13i3.36907

Cited by 19 publications

(22 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The powder of dried seeds of P. longifolia (1.0 kg) was extracted with absolute methanol at room temperature (3 times). The whole extract was evaporated under low pressure to obtain a methanolic extract (ME, 45 g, 4.5% w/w) 30 . By using column chromatography (CC) of mesh size 100-200 via a hexane/ethyl acetate solvent system (step gradient flow), ME extract (5.0 g) yielded five major fractions (F1-5).…”

Section: Extraction and Isolationmentioning

confidence: 99%

Dual COX and 5-LOX inhibition by clerodane diterpenes from seeds of Polyalthia longifolia (Sonn.) Thwaites

Nguyen

Chandi

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Natural metabolites with their specific bioactivities are being considered as a potential source of materials for pharmacological studies. In this study, we successfully isolated and identified five known clerodane diterpenes, namely 16-oxo-cleroda-3,13(14)E-dien-15-oic acid (1), 16-hydroxy-cleroda-3,13-dien-15-oic acid (2), 16-hydroxy-cleroda-4(18),13-dien-16,15-olide (3), 3α,16α-dihydroxy-cleroda-4(18),13(14)Z-dien-15,16-olide (4), and 16α-hydroxy-cleroda-3,13(14)Z-dien-15,16-olide (5) from the methanolic extract of seeds of Polyalthia longifolia. Initially, all the isolated metabolites were investigated for COX-1, COX-2, and 5-LOX inhibitory activities using the standard inhibitory kits. Of which, compounds 3, 4, and 5 exhibited to be potent COX-1, COX-2, and 5-LOX inhibitors with the IC50 values similar or lower to those of the reference drugs. To understand the underlying mechanism, these compounds were subjected to molecular docking on COX-1, COX-2, and 5-LOX proteins. Interestingly, the in silico study results were in high accordance with in vitro studies where compounds 3, 4, and 5 hits assumed interactions and binding pattern comparable to that of reference drugs (indomethacin and diclofenac), as a co-crystallized ligand explaining their remarkable dual (COX/LOX) inhibitor actions. Taken together, our findings demonstrated that compounds 3, 4, and 5 functioned as dual inhibitors of COX/5-LOX and can contribute to the development of novel, more effective anti-inflammatory drugs with minimal side-effects.

show abstract

Section: Extraction and Isolationmentioning

confidence: 99%

Dual COX and 5-LOX inhibition by clerodane diterpenes from seeds of Polyalthia longifolia (Sonn.) Thwaites

Nguyen

Chandi

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The antioxidant activity was assessed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay [9] in triplet. Initially, 100 µL of 100 µM of DPPH in ethanol was prepared and reacted with known concentrations i.e.…”

Section: Dpph Assaymentioning

confidence: 99%

CHEMICAL AND PHARMACOLOGICAL EVALUATION OF MANGLICOLOUS LICHEN GRAPHIS AJAREKARII PATW. & C. R. KULK.

Polimati

Patnaik

Tatipamula

2019

JST

Self Cite

View full text Add to dashboard Cite

The chemical examination of ethanolic extract of manglicolous lichen Graphis ajarekarii (Ga-Et) resulted in isolation of three known metabolites – chiodectonic acid (1), graphenone (2) and graphisquinone (3). All the isolates (1-3) and Ga-Et were screened against DPPH and superoxide free radicals, six different cancer cell lines and one normal human cell line (NHME). This work is the first report of antioxidant and cytotoxicity studies on the isolated metabolites (1-3).

show abstract

“…The metabolites (1-9) and RM-Ac was subjected to 1,1diphenyl-2-picrylhydrazyl (DPPH) assay in triplicate, and results are reported as percentage of inhibition of DPPH free radicals [17]. Initially, to the known concentrations of the sample added 0.004% DPPH dissolved in methanol and incubated for half-an-hour at 37°C.…”

Section: Antioxidant Activity Dpph Assaymentioning

confidence: 99%

“…In DPPH assay, reduction of the DPPH free radicals to a DPPH-H (non-radical) form by antioxidant capable substance takes place [17,19]. As shown in Fig.…”

Section: Antioxidant Activitymentioning

confidence: 99%

See 1 more Smart Citation

Chemical and pharmacological evaluation of manglicolous lichen Roccella montagnei Bel em. D. D. Awasthi

2019

Self Cite

View full text Add to dashboard Cite

Background: Lichen is a composite organism composed of fungus in association with algae or cyanobacteria. Particularly, lichen betide to mangroves are named as manglicolous lichens. From the folklore, lichen extracts were used in the management of many infections and diseases. Results: The chemical investigation of acetone extract of manglicolous lichen Roccella montagnei (RM-Ac) yielded nine known metabolites namely divarinolmonomethylether (1), ethyl divaricatinate (2), divarinol (3), orcinol (4), methyl 2,6-dihydroxy-4-methylbenzoate (5), haematommic acid (6), atranol (7), ethyl haematommate (8) and ethyl orsellinate (9). Except 4, all are for the first reported from this species. The RM-Ac and its metabolites (1-9) were screened for antioxidant (DPPH, ABTS and superoxide free radical assays), anti-inflammatory, anticancer (SRB assay using A549, DLD-1, FADU, HeLa and MCF-7) and acute toxicity studies. The pharmacological results showed that compounds 6 and 8 depicted potent inhibitory profile against ABTS free radical (with IC 50 value of 40.0 and 40.5 μg/mL, respectively) and protein denaturation (with IC 50 value of 435 and 403 μg/mL, respectively). LD 50 of RM-Ac was found to be above 2 g/Kg body weight. Moreover, the RM-Ac showed prominent inhibition of formalin-hind albino rat paw oedema at both the tested doses, i.e., 100 and 200 mg/Kg b.w than that of the standard drug (indomethacin). Furthermore, the compounds 6 and 8 exhibited significant degree of specificity against HeLa, FADU and A549; besides, they showed very little degree of specificity against NHME cell line specifying less toxicity to normal cells. Conclusion: It can be concluded that the manglicolous lichen R. montagnei has an aptitude to act against free radicals, inflammation and cancer, and the main metabolites responsible for its biological activity are 6 and 8.

show abstract

Protective effect of Aurelia aurita against free radicals and streptozotocin-induced diabetes

Cited by 19 publications

References 33 publications

Dual COX and 5-LOX inhibition by clerodane diterpenes from seeds of Polyalthia longifolia (Sonn.) Thwaites

Dual COX and 5-LOX inhibition by clerodane diterpenes from seeds of Polyalthia longifolia (Sonn.) Thwaites

CHEMICAL AND PHARMACOLOGICAL EVALUATION OF MANGLICOLOUS LICHEN GRAPHIS AJAREKARII PATW. & C. R. KULK.

Chemical and pharmacological evaluation of manglicolous lichen Roccella montagnei Bel em. D. D. Awasthi

Contact Info

Product

Resources

About