Protective Effect of Ajuga multiflora BUNGE Extract on Lead Toxicity of Environmental Pollutant

Abstract

To evaluate the protective effect of Ajuga multiflora BUNGE (AMB) extract on the toxicity of lead acetate (LA), environmental pollutant, cell viability was measured by XTT assay using cultured NIH3T3 fibroblasts. And also, the effect of antioxidant, butylated hydroxytoluene (BHT) on LA-induced cytotoxicity was analysed. For the protective effect of AMB extract on LA-induced cytotoxicity, NIH3T3 fibroblasts were pretreated with 80 or 90 μg/mL of AMB extract for 2 hours before the treatment of LA. And also, the antioxidative effects of AMB extract against LA-induced cytotoxicity were assessed by DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP). In this study, LA significantly decreased cell viability dose-dependently compared with control, and then XTT50 value was determined at 46.1 μM of LA. In the effect of BHT against LA-induced cytotoxicity, it effectively prevented toxic effect of LA by the significant increase of cell viability. In the protective effect of AMB extract on LA-induced cytotoxicity, it significantly increased cell viability which was decreased by LA-induced cytotoxicity, and also it showed the antioxidative effects such as DPPH-radical scavenging activity, SOD-like activity and inhibitory activity of LP. From these results, it is suggested that the cytotoxicity of LA is involved in oxidative stress, and AMB extract effectively prevented the cytotoxicity induced by LA via an antioxidative effect. Conclusively, the natural substance such as AMB extract may be alternative resources for the prevention or treatment of diseases related with oxidative stress.

The Antioxidative Effect ofEclipta prostrataL. Extract on Cultured NIH3T3 Fibroblasts Injured by Manganese-Induced Cytotoxicity

“…그러나 아직까지 망간 중독에 대한 효과적인 치료방법이 확립되어 있지 않을 뿐만 아니라 이 의 독성현상에 대한 병인기전도 자세히 밝혀지지 않았으 며 (Cho, 1991), 세포나 분자수준에서 망간의 피부독성평가 나 기전에 대한 연구는 많지 않다 (Fulle et al, 2013). 최근, 망간과 같은 중금속류인 수은이나 카드뮴, 크롬의 독성이 산화적 손상(oxidative stress)과 관련이 있다고 제시되면서 이의 독성에 자유라디칼(free radicals)이 관여하고 있음이 보고된 바 있다 (Son et al, 2017 (Jung et al, 2017a). 식물 중 한련초 (Eclipta prostrata L., EP)는 국화과(Compositae)에 속하는 한 해살이풀로 우리나라 전국에 분포하고 있으며, 주로 논두 렁이나 도랑 등 습지나 논밭주변에 자생하고 있다 (Chang, 2003).…”