The Antioxidative Effect ofEclipta prostrataL. Extract on Cultured NIH3T3 Fibroblasts Injured by Manganese-Induced Cytotoxicity

Abstract

Manganese (Mn) is used as main materials in various chemical processes of industry, but it suggested that Mn brings about its toxicant by fume or dust through respiratory system and skin barrier. Mn toxicant induces the loss of mental health and life quality by cerebrovascular and skin diseases. Nevertheless, it lefts much unknown on the mechanism and the effectively therapeutic methods about Mn toxicant. Therefore, this study was evaluated the cytotoxicity induced by manganese dioxide (MnO 2) in cultured NIH3T3 fibroblasts, and also, the correlation between MnO 2-induced cytotoxicity and oxidative stress was examined. While, the effect of Eclipta prostrata L. (EP) extract belong to Compositae was assessed against MnO 2-induced cytotoxicity in the view of antioxidative effect for searching the natural resources mitigating or preventing the MnO 2-induced cytotoxicity. In this study, MnO 2-induced cytotoxicity was revealed as mid-toxic by Borenfreud and Puerner's toxic criteria, and catalase (CAT), an antioxidant prevented MnO 2-induced cytotoxicity by the remarkable increase of cell viability in these cultures. While, in the protective effect of EP extract on MnO 2-induced cytotoxicity, EP extract effectively prevented the cytotoxicity induced by MnO 2 via antioxidative effects such as xanthine oxidase (XO) inhibitory ability and DPPH-radical scavenging ability. From the above results, EP extract showed the effective prevention against MnO 2-induced cytotoxicity correlated with oxidative stress by antioxidative effects. Conclusively, this study may be useful to research or development the alternatively therapeutic agent from natural resources like EP extract for the treatment of diseases resulted in oxidative stress.

Eclipta prostrata (L.) L. (uses and bioactivities)

“…The oxidative stress due to ultraviolet irradiation is directly or indirectly involved in various diseases. The bioactivity of E. prostrata as an antioxidant has been reported by Chen et al [17], Gani and Devi [2], and Lee et al [18]. The aqueous extract of E. prostrata had a potent effect in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radicals, and iron chelating ions with IC50 values of 0.23 mg/mL, 0.48 mg/mL, and 1.…”

“…Manganese (Mn) is one of the heavy metals used in various industrial chemical processes, but at certain levels Mn is toxic in the respiratory system and skin protection. Toxic Mn causes loss of mental health and quality of life by cerebrovascular and skin diseases [18]. The extract of E. prostrata effectively prevented the cytotoxicity caused by MnO2 through its antioxidant effects such as xanthine oxidase (XO) inhibitory ability and radical scavenging ability of DPPH [18].…”

“…Toxic Mn causes loss of mental health and quality of life by cerebrovascular and skin diseases [18]. The extract of E. prostrata effectively prevented the cytotoxicity caused by MnO2 through its antioxidant effects such as xanthine oxidase (XO) inhibitory ability and radical scavenging ability of DPPH [18]. EP has high antioxidant activity which is closely correlated with increased anti-inflammatory activity of the skin by effectively suppressing tumor production of necrosis factor-alpha and interleukin-6 from macrophages.…”