Protective effect of a novel herbmedicine, Hepad, on apoptosis of SH-SY5Y cells and a rat model of Parkinson’s disease

Baek, Seung Min; Lee, Na Rae; Kim, Da Hye; Gu, Ayoung; Kim, Seong Yeol; Song, Dae-Yong; Kim, Dong‐Hee; Choi, Hak Joo; Park, Byung‐Jun; Kim, In Sik

doi:10.1007/s13273-015-0021-7

Cited by 6 publications

(11 citation statements)

References 27 publications

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“…The crucial characteristics of parkinsonism, including impaired motor function, decreased dopamine content, and reduced number of dopaminergic neurons (cell bodies and axonal terminals) in the nigrostriatal system, were replicated here in MPTP-intoxicated mice. In previous studies, we reported the apoptotic mechanisms by which Hepad exerts its protective effects in cell and animal PD models induced by exposure to 6-hydroxydopamine (6-OHDA) [ 16 ]. In the present study, we prepared H1 by mixing Atractylodis Rhizoma , Cnidii Rhizoma , Paeonia japonica , Poria cocos Wolf , Uncariae Ramulus Et Uncus , and Zizyphi Semen [ 16 ] and H2 by mixing Paeonia japonica , Uncariae Ramulus et Uncus , and Machilus thunbergii .…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, we reported the apoptotic mechanisms by which Hepad exerts its protective effects in cell and animal PD models induced by exposure to 6-hydroxydopamine (6-OHDA) [ 16 ]. In the present study, we prepared H1 by mixing Atractylodis Rhizoma , Cnidii Rhizoma , Paeonia japonica , Poria cocos Wolf , Uncariae Ramulus Et Uncus , and Zizyphi Semen [ 16 ] and H2 by mixing Paeonia japonica , Uncariae Ramulus et Uncus , and Machilus thunbergii . To our knowledge, this is the first study to demonstrate that H1 and H2 exert their neuroprotective effects by regulating inflammation, anti-oxidant enzymes, and apoptosis in the SH-SY5Y cell and mouse PD models that are induced by MPTP intoxication.…”

Section: Discussionmentioning

confidence: 99%

“…Our research has focused on herbal medicines for the treatment of PD. In previous studies, herbal medicines, such as Hepad, which is composed of Atractylodis Rhizoma , Cnidii Rhizoma , Paeonia japonica , Poria cocos Wolf , Uncariae Ramulus et Uncus , and Zizyphi Semen , have been found to inhibit cell apoptosis by reducing activation of the caspase-9/caspase-3 pathway [ 16 ]. Regarding the biological effects of Hepad’s components, Atractylodis Rhizoma Alba has been found to exhibit anti-oxidative, gastroprotective, and anti-inflammatory activity [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Mitigation Effects of a Novel Herbal Medicine, Hepad, on Neuroinflammation, Neuroapoptosis, and Neuro-Oxidation

et al. 2018

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Parkinson’s disease (PD), a common adult-onset neurodegenerative disorder with complex pathological mechanisms, is characterized by the degeneration of dopaminergic nigrostriatal neurons. The present study demonstrated that the herbal medicines Hepad 1 and 2 protected against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic neurotoxicity in C57BL/6 mice and SH-SY5Y cells. Hepad 1 and 2 remarkably alleviated the enhanced expression of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2, macrophage-1, and phosphorylated iκB-α) and apoptotic signals (Bcl-2-associated X protein, caspase-3, and poly [ADP-ribose] polymerase-1). Additionally, Hepad reduced MPTP-induced oxidative damage by increasing the expression of anti-oxidant defense enzymes (superoxide dismutase and glutathione S-transferase) and downregulating the levels of nicotinamide adenine dinucleotide phosphate oxidase 4. This study also showed that the neuroprotective effects of Hepad include anti-inflammatory, anti-apoptotic, and anti-oxidative properties, in addition to activation of the protein kinase B, extracellular-signal-regulated kinase, and c-Jun N-terminal kinase signaling pathways. Furthermore, oral administration of Hepad 1 and 2 attenuated the death of tyrosine hydroxylase-positive substantia nigra neurons that was induced by 20 mg/kg MPTP. Therefore, our results suggest that Hepad 1 and 2 are useful for treating PD and other disorders associated with neuro-inflammatory, neuro-apoptotic, and neuro-oxidative damage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mitigation Effects of a Novel Herbal Medicine, Hepad, on Neuroinflammation, Neuroapoptosis, and Neuro-Oxidation

et al. 2018

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“…However, both trunk exercises in water and on land-based were found improve gait parameters in stroke patients. However, further systematic and scientific studies in neurorehabilitation and other fields are needed to confirm the effects of therapy 28 , 29 ) .…”

Section: Discussionmentioning

confidence: 99%

A comparative study of the effects of trunk exercise program in aquatic and land-based therapy on gait in hemiplegic stroke patients

et al. 2016

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[Purpose] The purpose of this study was to compare the effects of aquatic and land-based trunk exercise program on gait in stroke patients. [Subjects and Methods] The subjects were 28 hemiplegic stroke patients (20 males, 8 females). The subjects performed a trunk exercise program for a total of four weeks. [Results] Walking speed and cycle, stance phase and stride length of the affected side, and the symmetry index of the stance phase significantly improved after the aquatic and land-based trunk exercise program. [Conclusion] These results suggest that the aquatic and land-based trunk exercise program may help improve gait performance ability after stroke.

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“…Genotyping using polymerase chain reaction for DJ-1 −/− confirmation was performed with the following primers: DJ-1 forward, 5′-GCT GAA ACT GTG CCA TGT GA-3′; DJ-1 reverse, 5′-TGC TAA AGC GCA TGC TCC AGA CT-3′; Mutant Neo, 5′-TGG ATG TGG AAT GTG TGC GAG-3′. The expression of DJ-1 protein was confirmed in aortic strips using western blotting analysis 13 , 16 , 17 ) . Our investigation conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No.…”

Section: Methodsmentioning

confidence: 99%

Differences in strength-duration curves of electrical diagnosis by physiotherapists between DJ-1 homozygous knockout and wild-type mice: a randomized controlled pilot trial

et al. 2016

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[Purpose] Strength-duration (SD) curves are used in electrical diagnosis by physiotherapists to confirm muscle degeneration. However, the usefulness of SD curves in comparing muscle degeneration in DJ-1 homozygous knockout (DJ-1−/−) and wild-type mice (DJ-1+/+) is not yet fully understood. The electrical properties of the gastrocnemius muscles of DJ-1−/− and DJ-1+/+ mice were compared in the current study. [Subjects and Methods] The electrode of an electrical stimulator was applied to the gastrocnemius muscle to measure the rheobase until the response of contractive muscle to electrical stimulation became visible in mice. [Results] The rheobase of DJ-1−/− mice showed a significant increase in a time-dependent manner, compared to that of DJ-1+/+ mice. [Conclusion] These results demonstrate that the DJ-1 protein may be implicated in the regulation of neuromuscular activity of gastrocnemius muscles of mice.

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Protective effect of a novel herbmedicine, Hepad, on apoptosis of SH-SY5Y cells and a rat model of Parkinson’s disease

Cited by 6 publications

References 27 publications

Mitigation Effects of a Novel Herbal Medicine, Hepad, on Neuroinflammation, Neuroapoptosis, and Neuro-Oxidation

Mitigation Effects of a Novel Herbal Medicine, Hepad, on Neuroinflammation, Neuroapoptosis, and Neuro-Oxidation

A comparative study of the effects of trunk exercise program in aquatic and land-based therapy on gait in hemiplegic stroke patients

Differences in strength-duration curves of electrical diagnosis by physiotherapists between DJ-1 homozygous knockout and wild-type mice: a randomized controlled pilot trial

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