Protective effect of a hydrogel containing Achyrocline satureioides extract-loaded nanoemulsion against UV-induced skin damage

Balestrin, Lucélia Albarello; Bidone, Juliana; Bortolin, Rafael Calixto; Moresco, Karla Suzana; Moreira, José Cláudio Fonseca; Teixeira, Hélder Ferreira

doi:10.1016/j.jphotobiol.2016.08.039

Cited by 42 publications

(38 citation statements)

References 36 publications

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“…All formulations presented results of droplet size (nm) in accordance with nanoemulsions obtained by the spontaneous emulsification procedure, ranging from 170 to 250 nm . Additionally, monodisperse formulations were achieved, exhibiting a PDI lower than 0.3 . It was also observed that formulations containing Tween ® 80 presented a droplet size and PDI significantly lower ( P < 0.05) than others.…”

Section: Resultssupporting

confidence: 62%

“…These results can be easily explained due to the higher viscosity of the formulations containing a thickening agent, hydroxyethyl cellulose polymer. This nonionic polymer acts as an additional diffusional barrier to RA release from the formulations, delaying the active molecule release and extending the presence of RA, expected for topical application …”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, RA presents some drawbacks that can be relevant causes for the product's loss of effectiveness, such as poor water solubility, high instability and poor permeability through biological barriers . For this reason, technological approaches to circumvent these problems have been studied, such as the use of lipid‐nanotechnology‐based delivery systems . Our research group has recently been interested in the development of topical nanoemulsions as delivery systems for RA.…”

Section: Introductionmentioning

confidence: 99%

“…Our research group has recently been interested in the development of topical nanoemulsions as delivery systems for RA. Nanoemulsions are dispersions of two immiscible liquids (oil‐in‐water) stabilized by an appropriate surfactant system, that have been widely studied as a promising alternative for the treatment of skin diseases and for cosmetics purposes, since their small droplet sizes can improve active lipophilic molecules penetration through the skin, increasing their topical effect . Nevertheless, for topical application, the low viscosity from nanoemulsions is a concern and the design of a semisolid formulation is a key consideration.…”

Section: Introductionmentioning

confidence: 99%

“…[5,8] For this reason, technological approaches to circumvent these problems have been studied, such as the use of lipid-nanotechnologybased delivery systems. [18][19][20][21][22] Our research group has recently been interested in the development of topical nanoemulsions as delivery systems for RA. Nanoemulsions are dispersions of two immiscible liquids (oil-in-water) stabilized by an appropriate surfactant system, that have been widely studied as a promising alternative for the treatment of skin diseases and for cosmetics purposes, since their small droplet sizes can improve active lipophilic molecules penetration through the skin, increasing their topical effect.…”

Section: Introductionmentioning

confidence: 99%

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Development, physico-chemical characterization and in-vitro studies of hydrogels containing rosmarinic acid-loaded nanoemulsion for topical application

Marafon

Fachel

Prá

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives Natural sources with antioxidant activity, such as rosmarinic acid (RA), have been considered as an interesting approach for the development of new anti‐ageing skin products. In this context, this study aimed to develop hydrogels containing RA‐loaded nanoemulsions and evaluate the effect of the addition of Tween® 80 (a nonionic cosurfactant) in formulations intended to be used for topical application. Methods Physico‐chemical characterization, in‐vitro release and skin retention/permeation from hydrogels of RA‐loaded nanoemulsions (containing or not Tween® 80) were evaluated. The RA‐loaded nanoemulsion safety profiles were also investigated in keratinocytes (HaCaT cells). Key findings It was observed that all formulations presented adequate physico‐chemical characterization for topical application. Furthermore, the results also demonstrated that the presence of Tween® 80 decreased the droplet size and polydispersity index of nanoemulsions and hydrogels. An extended RA release was noted for the hydrogels. However, when comparing the hydrogels, a positive effect of the presence of Tween® 80 on RA retention/permeation in total skin was seen. The RA‐loaded nanoemulsion safety profiles demonstrated a good tolerability (3.125–100 μm) in HaCaT cells. Conclusions The overall results demonstrated that the formulations developed in this study can be considered as a suitable carrier for RA in a topical application targeting new anti‐ageing skin care products.

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Section: Resultssupporting

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development, physico-chemical characterization and in-vitro studies of hydrogels containing rosmarinic acid-loaded nanoemulsion for topical application

Marafon

Fachel

Prá

et al. 2019

Journal of Pharmacy and Pharmacology

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A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

Balestrin

Fachel

Koester

et al. 2020

Phytochemical Analysis

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Introduction: Achyrocline satureioides (marcela or macela) is a plant widely used in folk medicine in South America. Recently, there has been increasing interest for the development of skin care products containing A. satureoides extracts, due to its welldocumented antioxidant, antiherpetic, and wound healing properties. Objectives: The present study aimed to develop and validate a yet unexplored stability-indicating and robust ultra-fast liquid chromatography (UFLC) method for the simultaneous quantification of the main flavonoids of A. satureioides in extracts, nanoemulsions, and porcine skin layers. Material and methods: The chromatographic separation of flavonoids quercetin, luteolin, and 3-O-methylquercetin was performed on a Luna C18 analytical column (100 mm × 2.0 mm i.d.; particle size 2.5 μm) using isocratic elution with methanol/phosphoric acid 1% (48:52 v/v) with a flow rate of 0.3 mL/min at 40 C. Results: The method was found to be specific, linear (R > 0.998), precise, accurate, and robust for all flavonoids assayed in A. satureioides extract, nanoemulsions, and porcine ear skin. A low matrix effect was noted for all complex matrices. The stability-indicating UFLC method was evaluated by submitting isolated flavonoids, a mixture of standards, and A. satureioides extract to acidic, alkaline, oxidative, UV-A/UV-C light, and thermal stress conditions. No peaks were found co-eluting with the flavonoids of interest in all matrices. The robustness of the method was confirmed using Plackett-Burman experimental design. Conclusion: The short run time (8 min) and reliability of the method could be useful for the determination of A. satureioides flavonoids in topical product development since extracts of this medicinal plant have been used to treat various skin disorders.

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Bio-Based Nanoemulsion Formulations Applicable in Agriculture, Medicine, and Food Industry

Jampílek

Kráľová

Campos

et al. 2019

Nanotechnology in the Life Sciences

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Nanotechnology providing "a new dimension" accompanied with new properties conferred to many current materials is widely used for production of a new generation of agrochemicals, in medicine it enables improved drug bioavailability, reducing undesirable side effects, minimizing non-specific uptake and specific targeting to certain target cells, while in food industry it has great importance in food protection and biofortification of food with valuable ingredients. In biobased nanoemulsions belonging to lipid nanocarriers plant oils used for oil phase, emulsifiers, biosurfactants, cosurfactants, targeting ligands on the surface of nanoemulsion (e.g., folate) or encapsulated active ingredients are of natural origin. The biocomponents of such nanoemulsions show low toxicity to living organisms, could protect encapsulated compounds for degradation, ensure their sustainable release and reduce the amount of active ingredient necessary for required effect. This chapter presents a comprehensive current overview of recent findings in the field of nanoemulsions and their utilization in agriculture and food industry, with the main emphasis on formulations encapsulating essential oils or plant extracts suitable as effective pesticide preparations as well as medicinal applications of bio-based nanoemulsions, where attention is paid to transdermal nanoemulsion formulations, the use of nanoemulsions in cancer therapy and for pulmonary and ocular drug delivery. Nanoemulsions formulated with natural emulsifiers, biosurfactants and biopolymers are presented and bio-based nanoemulsions of essential oils and their constituents as well as nanoemulsions with encapsulated vitamins, fatty acids and some bioactive compounds are discussed. Applications of nanoemulsions in edible coatings are outlined as well.

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Protective effect of a hydrogel containing Achyrocline satureioides extract-loaded nanoemulsion against UV-induced skin damage

Cited by 42 publications

References 36 publications

Development, physico-chemical characterization and in-vitro studies of hydrogels containing rosmarinic acid-loaded nanoemulsion for topical application

Development, physico-chemical characterization and in-vitro studies of hydrogels containing rosmarinic acid-loaded nanoemulsion for topical application

A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

Bio-Based Nanoemulsion Formulations Applicable in Agriculture, Medicine, and Food Industry

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