Protective effect of 2-dodecyl-6-methoxycyclohexa-2, 5-diene-1, 4-dione, isolated from Averrhoa carambola L., against Aβ1–42-induced apoptosis in SH-SY5Y cells by reversing Bcl-2/Bax ratio

Lu, Sisi; Wei, Xiaojie; Zhang, Hongliang; Chen, Zhen-Feng; Li, Juman; Xu, Xiaogang; Xie, Qiuqiao; Chen, Lixiu; Ye, Fangxing; Phama, Hoa Thi Thai; Jiang, Liping; Huang, Tianmin; Wei, Jinbin; Huang, Renbin

doi:10.1007/s00213-020-05668-9

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…The Bcl-2 family consists of pro-apoptotic proteins (Bax, Bad, and Bak) and anti-apoptotic proteins (Bcl-2 and Bcl-XL) (Pahrudin Arrozi et al., 2020 ). Bax can increase mitochondrial permeability, release pro-apoptotic factors, and inhibit anti-apoptotic Bcl-2 family proteins to trigger caspase signaling, leading to neuronal apoptosis (Lu et al., 2021 ). In order to evaluate the inhibitory effect of varying formulations on nerve cell apoptosis in vivo , the paraffin sections of brain tissue treated with free ICA/TSIIA, ICA/TSIIA liposomes or Ang2-ICA/TSIIA liposomes were stained by immunohistochemistry.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional icariin and tanshinone IIA co-delivery liposomes with potential application for Alzheimer’s disease

et al. 2022

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The blood–brain barrier (BBB) is a protective barrier for brain safety, but it is also a major obstacle to the delivery of drugs to the cerebral parenchyma such as the hippocampus, hindering the treatment of central nervous system diseases such as Alzheimer’s disease (AD). In this work, an anti-AD brain-targeted nanodrug delivery system by co-loading icariin (ICA) and tanshinone IIA (TSIIA) into Aniopep-2-modified long-circulating (Ang2-ICA/TSIIA) liposomes was developed. Low-density lipoprotein receptor-related protein-1 (LRP1) was a receptor overexpressed on the BBB. Angiopep-2, a specific ligand of LRP1, exhibited a high binding efficiency with LRP1. Additionally, ICA and TSIIA, drugs with neuroprotective effects are loaded into the liposomes, so that the liposomes not only have an effective BBB penetration effect, but also have a potential anti-AD effect. The prepared Ang2-ICA/TSIIA liposomes appeared narrow dispersity and good stability with a diameter of 110 nm, and a round morphology. Cell uptake observations, BBB models in vitro , and imaging analysis in vivo showed that Ang2-ICA/TSIIA liposomes not only penetrate the BBB through endocytosis, but also accumulate in N2a cells or brain tissue. The pharmacodynamic analysis in vivo demonstrated that Ang2-ICA/TSIIA liposomes could improve AD-like pathological features in APP/PS1 mice, including inhibiting neuroinflammation and oxidative stress, reducing apoptosis, protecting neurons, and improving cognitive function. Therefore, Ang2-ICA/TSIIA liposomes are considered a potentially effective therapeutic strategy for AD.

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Section: Resultsmentioning

confidence: 99%

Multifunctional icariin and tanshinone IIA co-delivery liposomes with potential application for Alzheimer’s disease

et al. 2022

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“…As previously reported [ 13 ], Bcl-2 and Bax are a pair of antagonistic partners and play opposite roles in the mitochondrial apoptosis pathway. The ratio of Bcl-2/Bax could reflect cell proliferation and apoptosis [ 41 ]. In our study, Bcl-2, Bcl-xl, Bax, and Bim were detected by western blot, and the results showed that both Bcl-2 and Bcl-xl were decreased in the VCD group but were rescued in the DBT group.…”

Section: Discussionmentioning

confidence: 99%

Danggui Buxue Tang Rescues Folliculogenesis and Ovarian Cell Apoptosis in Rats with Premature Ovarian Insufficiency

Wang

Liu

Nie

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Premature ovarian insufficiency (POI) is a common female endocrine disease that is closely linked to ovarian function. Danggui Buxue Tang (DBT) is a classic prescription of traditional Chinese medicine that is helpful for rescuing ovarian function. However, the mechanism by which DBT rescues ovarian function remains unclear. This study explored the molecular mechanism of DBT with respect to apoptosis and related signals in ovarian cells. The quality control of DBT was performed by HPLC. After DBT intervention in the POI rat model, serum AMH/FSH/LH/E2 levels were detected by ELISA, follicles at various developmental stages were observed by HE staining, apoptosis was detected by TUNEL, and the expression profiles of Bcl-2 family proteins and key proteins in the Jak2/Foxo3a signaling pathway were evaluated by western blot. The results demonstrated that DBT could encourage the development of primary/secondary/antral follicles and increase the secretion of AMH. Moreover, DBT might inhibit Foxo3a by upregulating Jak2, thereby mediating Bcl-2 family activities and inhibiting apoptosis in ovarian cells. In conclusion, DBT promotes follicular development and rescues ovarian function by regulating Bcl-2 family proteins to inhibit cell apoptosis, which could be related to the Jak2/Foxo3a signaling pathway.

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“…Studies have suggested that the activation of the PI3K/Akt signaling pathway may prevent Aβ-induced neurotoxicity [ [ 50 ]]. The ratio of Bcl-2 to Bax is known to be correlated with apoptosis [ [ 51 ]]. To investigate the neuroprotective mechanisms of adlay hull extracts, the ratio of Bcl-2/Bax in d-PC12 cells was measured by western blotting, and AHWE was found to significantly activate PI3K/Akt signaling, upregulate the expression of the antiapoptotic protein Bcl-2, and suppress the expression of the pro-apoptotic protein Bax.…”

Section: Discussionmentioning

confidence: 99%

Adlay hull extracts attenuate β-amyloid-induced neurotoxicity and oxidative stress in PC12 cells through antioxidative, anti-inflammatory, and antiapoptotic activities

Tsay

Lin

Hsu

et al. 2021

Biochemistry and Biophysics Reports

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Alzheimer's disease (AD) is characterized by accumulation of β-amyloid (Aβ) in senile plaques, contributing to oxidative stress, mitochondrial diseases, and synaptic atrophy, consequently leading to the deterioration of brain function. Adlay ( Coix lacryma-jobi L.) is an annual botanical. Here, a 95% ethanol extract of adlay hull (AHEE) was partitioned by ethyl acetate (AHEAE), n -butanol (AHBUE), and water (AHWE), and the effects of these extracts on lipopolysaccharide (LPS)-induced RAW264.7 cells and Aβ-induced PC12 cells, as experimental models of neurotoxicity, were evaluated. The expression of anti-inflammatory and antiapoptosis-related proteins was investigated and AHEE, AHEAE, and AHWE were found to exert anti-inflammatory effects. AHWE exhibited antiapoptotic effects and inhibited inducible nitric oxide synthase expression and nitric oxide production. We investigated the protective effects of AHWE against Aβ-induced neurotoxicity in dPC12 cells and explored the underlying mechanism. Pretreatment with AHWE significantly attenuated cell death and Aβ-mediated increase in B cell lymphoma (Bcl)-2/Bax ratio. AHWE significantly inhibited Aβ and enhanced protein kinase B (Akt) level in dPC12 cells, suggesting that its protective effect against Aβ-induced apoptosis in dPC12 cells was mediated through upregulation of the phosphoinositide 3-kinases (PI3K)/Akt signaling pathway. These extracts and its bioactive compound K36–21 may be potentially useful to treat neurodegenerative disorders.

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Protective effect of 2-dodecyl-6-methoxycyclohexa-2, 5-diene-1, 4-dione, isolated from Averrhoa carambola L., against Aβ1–42-induced apoptosis in SH-SY5Y cells by reversing Bcl-2/Bax ratio

Cited by 11 publications

References 27 publications

Multifunctional icariin and tanshinone IIA co-delivery liposomes with potential application for Alzheimer’s disease

Multifunctional icariin and tanshinone IIA co-delivery liposomes with potential application for Alzheimer’s disease

Danggui Buxue Tang Rescues Folliculogenesis and Ovarian Cell Apoptosis in Rats with Premature Ovarian Insufficiency

Adlay hull extracts attenuate β-amyloid-induced neurotoxicity and oxidative stress in PC12 cells through antioxidative, anti-inflammatory, and antiapoptotic activities

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