Protective and anti-inflammatory effects of hydroalcoholic leaf extract of <i>Origanum vulgare</i> on oxidative stress, <i>TNF-α</i> gene expression and liver histological changes in paraquat-induced hepatotoxicity in rats

Sharifi-Rigi, Ali; Heidarian, Esfandiar; Amini, Sayed Asadollah

doi:10.1080/13813455.2018.1437186

Cited by 39 publications

(27 citation statements)

References 34 publications

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“…Our results are in agreement with these reports and we have demonstrated that HYP is associated with enhanced oxidative stress and hepatotoxicity. Therapeutic interventions of natural origin which are capable of attenuating oxidative stress are known to exert hepatoprotective and other beneficial effects [22][23][24]. Our findings are in line with these reports and we demonstrated that hydroalcoholic extract of S. chirata possesses strong antioxidant potential as indicated by the reduced ROS generation, improved catalase activity, enhanced GSH levels, and inhibition of lipid peroxidation (Fig.…”

Section: Discussionsupporting

confidence: 91%

Antioxidant and Hepatoprotective Effect of Swertia Chirata on Hypoxia-Induced Oxidative Stress in Wistar Rats

Kaushal

Singh

Thakur

2019

Asian J Pharm Clin Res

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Objective: Swertia chirata has been used in traditional and folklore medicine to treat several ailments such as hepatic disorders. However, the mechanistic and experimental justification to its traditional use is lacking. The present study was aimed to investigate the hepatoprotective potential of S. chirata during hypoxia (HYP)-induced hepatic damage in Wistar rats and to determine the underlying mechanism.Methods: Hydroalcoholic extract of S. chirata was prepared using Soxhlet extraction. Animals were divided into six groups (n=5). Animals in the HYP groups were subjected to HYP for 3 days (10% O2) to induce oxidative stress and hepatic damage. 50 and 100 mg/kg extract treatments were provided orally once daily for 7 days after which animals were sacrificed, and biochemical investigations for oxidative stress, liver function tests, and hepatic histopathology were performed.Results: HYP-induced marked oxidative stress as indicated by the significantly elevated mitochondrial ROS generation, lipid peroxidation, glutathione, and depleted catalase levels. Liver function test indicated hepatic damage as the levels of serum glutamic-oxaloacetic transaminase, serum glutamic pyruvic transaminase, and aspartate transaminase were significantly elevated in HYP animals. S. chirata treatment alleviated oxidative stress and improved liver functions in a dose-dependent manner. Liver histopathology confirmed the marked hepatic damage induced by HYP and revealed that S. chirata efficiently rescued liver from hypoxic damage.Conclusion: Hydroalcoholic extract of S. chirata is a potent hepatoprotective intervention which was associated with its potential to alleviate oxidative stress and improve liver functions. Moreover, it could find clinical application as a safer and alternative remedy for liver ailments.

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Section: Discussionsupporting

confidence: 91%

Antioxidant and Hepatoprotective Effect of Swertia Chirata on Hypoxia-Induced Oxidative Stress in Wistar Rats

Kaushal

Singh

Thakur

2019

Asian J Pharm Clin Res

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“…In treated rats with NAC and silymarin, however, the serum levels of GOT and GPT decreased compared with the second group (Table 2). Previous studies had shown that antioxidants can keep integrity of cell membrane and decrease exudation of enzymes through free radical scavenging activities (Giridharan & Sabina, 2017;Sharifi-Rigi et al, 2019). Therefore, the Furthermore, DIC has been reported to decline the activity of antioxidant enzymes such as SOD and CAT (Giridharan & Sabina, 2017;Nouri, Heidarian, & Nikoukar, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In treated rats with NAC and silymarin, however, the serum levels of GOT and GPT decreased compared with the second group (Table ). Previous studies had shown that antioxidants can keep integrity of cell membrane and decrease exudation of enzymes through free radical scavenging activities (Giridharan & Sabina, ; Sharifi‐Rigi et al, ). Therefore, the reduction of serum GPT and GOT activities, at least in part, may be resulted from the antioxidant activity of NAC on the cell membrane in DIC‐toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…Today, many studies have focused on the natural extract of herbs and dietary supplements to prevent the toxicity of various chemicals (Baravalia, Vaghasiya, & Chanda, ; Sharifi‐Rigi, Heidarian, & Amini, ; Valipour, Heidarian, Khoshdel, & Gholami‐Arjenaki, ). Cellular thiol modulation is a new therapeutic strategy for pathological injuries by reactive oxygen species (ROS) (Rhodes & Braakhuis, ).…”

Section: Introductionmentioning

confidence: 99%

“…Today, many studies have focused on the natural extract of herbs and dietary supplements to prevent the toxicity of various chemicals (Baravalia, Vaghasiya, & Chanda, 2011;Sharifi-Rigi, Heidarian, & Amini, 2019;Valipour, Heidarian, Khoshdel, & Gholami-Arjenaki, 2016).…”

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confidence: 99%

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Ameliorative effects ofN‐acetyl cysteine on diclofenac‐induced renal injury in male rats based on serum biochemical parameters, oxidative biomarkers, and histopathological study

Nouri

Heidarian

2019

J Food Biochem

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Diclofenac (DIC) can cause nephrotoxicity in humans. In this study, we evaluated the protective effects of N‐acetyl cysteine (NAC) on DIC‐induced nephrotoxicity. Rats were assigned to four groups. Group 1 was control group; group 2 administrated with DIC only; group 3 administrated with DIC plus NAC and group 4 was treated with DIC and silymarin. Then, the oxidative biomarkers in serum and kidney were evaluated. In group 2, DIC caused a remarkable elevation (p < 0.05) in the levels of serum uric acid, TNF‐α, creatinine, urea, GOT, and GPT, protein carbonyl, malondialdehyde (MDA), and renal TNF‐α gene expression, relative to control group. In treated groups with NAC and silymarin, a noticeable reduction (p < 0.05) was seen in mentioned levels of biochemical parameters. NAC showed that it could reduce the abnormality of biochemical parameters and histopathological changes which is induced by DIC. Practical applications N‐acetyl cysteine (NAC) has a potential to ameliorate renal histopathological changes and improving renal activity of antioxidant enzymes in nephrotoxicity by diclofenac. Also, NAC has a potential to reduce inflammatory gene expression in the diclofenac‐induced nephrotoxicity. Additionally, NAC can be considered as an antioxidant which reduces renal MDA and serum protein carbonyl due to nephrotoxicity by diclofenac.

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A behavioral and molecular study; ameliorated anxiety‐like behavior and cognitive dysfunction in a rat model of chronic unpredictable stress treated with oregano extract

et al. 2022

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Objective Chronic stress is considered a severe risk factor leading to various disorders, including anxiety and cognitive decline. The present study aimed to investigate the effects of Origanum vulgare (oregano) extract on improving anxiety‐like behavior and learning and memory defection caused by chronic unpredictable stress (CUS). Method A 10‐day CUS protocol was executed on male rats, and on day 10, their anxiety, learning, and memory status were evaluated. After that, in addition to the CUS, the rats were treated with the oregano extract for 2 weeks. Then, the expression of BDNF, TrkB, and TLR2/4 genes in the hippocampus and prefrontal cortex of the rats was evaluated. Also, the liver‐ and kidney‐related serum parameters, including triglycerides, total cholesterol, HDL, LDL, creatinine, urea, serum glucose, alanine aminotransferase, and aspartate aminotransferase were assessed. Further, the extract's lethal effect and its impact on animals’ body weight were investigated. Results Behavioral tests confirmed the anxiety‐like behavior and learning–memory function impairment caused by CUS. In contrast, the administration of the extract could significantly alleviate the mental deficiencies and diminished anxiety‐like behaviors. Molecular assessments showed that CUS could markedly decrease the BDNF and TrkB genes’ expression levels while increasing that of TLR2 and TLR4. In contrast, in extract‐treated animals, mRNA levels of BDNF and TrkB considerably increased, yet TLR2 and TLR4 mRNA levels reduced. Additionally, consumption of the extract caused weight gain, while having no lethality and detrimental effect on the liver and kidneys functions. Conclusions These findings indicate the anxiolytic properties of the extract and its improving effect on cognitive dysfunction.

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Protective and anti-inflammatory effects of hydroalcoholic leaf extract of Origanum vulgare on oxidative stress, TNF-α gene expression and liver histological changes in paraquat-induced hepatotoxicity in rats

Abstract: O. vulgare leaf extract has inhibitory effects on paraquat-induced liver damage due to its antioxidant properties.

Cited by 39 publications

References 34 publications

Antioxidant and Hepatoprotective Effect of Swertia Chirata on Hypoxia-Induced Oxidative Stress in Wistar Rats

Antioxidant and Hepatoprotective Effect of Swertia Chirata on Hypoxia-Induced Oxidative Stress in Wistar Rats

Ameliorative effects ofN‐acetyl cysteine on diclofenac‐induced renal injury in male rats based on serum biochemical parameters, oxidative biomarkers, and histopathological study

A behavioral and molecular study; ameliorated anxiety‐like behavior and cognitive dysfunction in a rat model of chronic unpredictable stress treated with oregano extract

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