Protective Activities of the Filamentous Hemagglutinin and the Lymphocytosis-Promoting Factor of Bordetella pertussis in Mice

Abstract

Protective activities of the filamentous hemagglutinin (FHA) and the lymphocytosis-promoting factor (LPF) of Bordetella pertussis were compared by active and passive protection tests with intracerebral or respiratory challenge in mice. Mice immunized twice by intraperitoneal injection of 8 micrograms of FHA or glutaraldehyde-inactivated LPF were protected after aerosol challenge. One intraperitoneal injection of inactivated LPF also protected mice from intracerebral challenge; the dose protecting 50% of the mice was 8.5 micrograms. However, one intraperitoneal injection of 48 micrograms of FHA or two weekly intraperitoneal injections of 20 micrograms did not protect mice from death after intracerebral challenge. Injection of affinity-purified antibody to LPF from mouse hybridomas or from goats gave a dose-dependent protection against aerosol challenge. The smallest dose giving protection was 80-90 micrograms. Polyclonal or monoclonal antibody to FHA at doses of 1,440 micrograms or 360 micrograms, respectively, gave very little protection from disease after respiratory challenge. These data indicate that active immunization of mice followed by respiratory challenge with B. pertussis is a useful model to identify protective antigens.

Eighty-Kilodalton N-Terminal Moiety of Bordetella pertussis Filamentous Hemagglutinin: Adherence, Immunogenicity, and Protective Role

“…Although several studies have demonstrated that FHA is able to induce protective immunity in murine models (4,5,19,35,36), which is the basis for its inclusion in most of the current ACVs (29), attempts to protect by passive transfer of anti-FHA antibodies have not always been successful (26,27). In most of these studies, the antibodies were administered intraperitoneally, whereas in this study, the i.v.…”

Reciprocal Protective Immunity againstBordetella pertussisandBordetella parapertussisin a Murine Model of Respiratory Infection

“…Infection by aerosols of B. pertussis or B. parapertussis was achieved by a modified version of the method of Oda et al (21,30,31). B. pertussis or B. parapertussis was cultured on BG plates for 30 h at 37°C.…”

Pertussis: the disease and new diagnostic methods

“…In mouse studies, FHA protects against aerosol challenge by active but not passive immunization (119,136). Immunization with FHA in concert with PT has been shown to have a synergistic effect (136).…”