Eighty-Kilodalton N-Terminal Moiety of
            <i>Bordetella pertussis</i>
            Filamentous Hemagglutinin: Adherence, Immunogenicity, and Protective Role

Alonso, Sylvie; Reveneau, Nathalie; Pethe, Kévin; Locht, Camille

doi:10.1128/iai.70.8.4142-4147.2002

Cited by 32 publications

(29 citation statements)

References 47 publications

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“…Heparan sulfates are one of the receptors for FHA (10) and are ubiquitous on the epithelial cell surface. In agreement with the observed protection in the animal model, Alonso et al (1) previously reported that mice immunized with the FHA fragment containing the complete HBD domain (Fha44) were protected against B. pertussis challenge. In summary, an scFv phage display library against B. pertussis antigens was constructed.…”

Section: Vol 75 2007supporting

confidence: 67%

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Construction and Characterization of Single-Chain Variable Fragment Antibodies Directed against the Bordetella pertussis Surface Adhesins Filamentous Hemagglutinin and Pertactin

et al. 2007

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Section: Vol 75 2007supporting

confidence: 67%

“…However, anti-FHA antibodies were found in sera from convalescent patients and vaccinated infants (20). In animal models, a number of laboratories, including ours, showed that antibodies to FHA or FHA fragments are protective (1,5,6,8,11,29,30).…”

mentioning

confidence: 99%

Construction and Characterization of Single-Chain Variable Fragment Antibodies Directed against the Bordetella pertussis Surface Adhesins Filamentous Hemagglutinin and Pertactin

et al. 2007

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“…2B). This result indicates that the fusion of HtrA to the C terminus of Fha44 overcomes the previously described defect in colonization of a B. pertussis strain producing Fha44 instead of FHA in a PTXdeficient background (2). Adherence studies using the human pulmonary epithelial cell line A549 (Fig.…”

Section: Resultsmentioning

confidence: 84%

“…However, when both PTX and FHA are lacking, col- onization is strongly impaired. The replacement of FHA with Fha44 in a PTX-deficient background did not restore the ability of the strain to colonize the respiratory tract at wild-type levels (2). Surprisingly, the fusion of HtrA to Fha44 in a PTXdeficient background partially restored the ability of the strain to colonize mouse lungs.…”

Section: Discussionmentioning

confidence: 90%

Production of Nontypeable Haemophilus influenzae HtrA by Recombinant Bordetella pertussis with the Use of Filamentous Hemagglutinin as a Carrier

et al. 2005

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Bordetella pertussis, the etiologic agent of whooping cough, is a highly infectious human pathogen capable of inducing mucosal and systemic immune responses upon a single intranasal administration. In an attenuated, pertussis toxin (PTX)-deficient recombinant form, it may therefore constitute an efficient bacterial vector that is particularly well adapted for the delivery of heterologous antigens to the respiratory mucosa. Filamentous hemagglutinin (FHA) has been used as a carrier to present foreign antigens at the bacterial surface, thereby inducing local, systemic, and protective immune responses to these antigens in mice. Both full-length and truncated (Fha44) forms of FHA have been used for antigen presentation. To investigate the effect of the carrier (FHA or Fha44) on antibody responses to passenger antigens, we genetically fused the HtrA protein of nontypeable Haemophilus influenzae to either FHA form. The fha-htrA and Fha44 gene-htrA hybrids were expressed as single copies inserted into the chromosome of PTX-deficient B. pertussis. Both chimeras were secreted into the culture supernatants of the recombinant strains and were recognized by anti-FHA and anti-HtrA antibodies. Intranasal infection with the strain producing the FHA-HtrA hybrid led to significantly higher antiHtrA and anti-FHA antibody titers than those obtained in mice infected with the Fha44-HtrA-producing strain. Interestingly, the B. pertussis strain producing the Fha44-HtrA chimera colonized the mouse lungs more efficiently than the parental, Fha44-producing strain and gave rise to higher anti-FHA antibody titers than those induced by the parental strain.The mucosal delivery of vaccines has several advantages over systemic delivery. Consequently, the development of efficient mucosal vaccine delivery systems, including live attenuated or commensal bacterial vaccines, has attracted much attention over the last decades. However, most efforts have so far been devoted to the oral route, although the oral delivery of vaccine formulations requires large quantities of antigen and repeated administrations to be effective. The nasal route may represent an interesting alternative (23), as it avoids the encounter with an acidic and proteolytic environment as well as the competition with commensal microorganisms present in the digestive tract.Bordetella pertussis, the etiologic agent of whooping cough, has recently been used as a live vector to deliver foreign antigens to the respiratory mucosa in laboratory models (29). As a highly contagious agent, it colonizes the human respiratory tract very efficiently and induces both local and systemic immune responses. The deletion of the genes encoding pertussis toxin (PTX), the major virulence factor of B. pertussis (24), has led to a highly attenuated strain, as evidenced by a strong reduction in lung inflammation and lymphocytosis (18). In this genetically attenuated form, B. pertussis is able to induce strong protection against respiratory challenges with virulent strains when given by the nasal route in a...

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“…Subsequent bacterial entry into host cells confers protection from the host immune system and may contribute to host tissue damage (4,13,15). Hemagglutinins can function as adhesins and are required for virulence of several bacterial pathogens (1,9,24,35). Hemagglutinins are also considered important virulence factors, as they can be a mechanism to acquire hemin, necessary for bacterial growth, from erythrocytes (39).…”

mentioning

confidence: 99%

Hemagglutinin B Is Involved in the Adherence of Porphyromonas gingivalis to Human Coronary Artery Endothelial Cells

et al. 2005

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Porphyromonas gingivalis is a periodontopathogen that may play a role in cardiovascular diseases. Hemagglutinins may function as adhesins and are required for virulence of several bacterial pathogens. The aim of this study was to determine the role of hemagglutinin B (HagB) in adherence of P. gingivalis to human coronary artery endothelial (HCAE) cells. P. gingivalis strain 381, a P. gingivalis 381 HagB mutant, Escherichia coli JM109 expressing HagB (E. coli-HagB), and E. coli JM109 containing pUC9 (E. coli-pUC9) were tested for their ability to attach to HCAE cells. Inhibition assays were performed to determine the ability of purified recombinant HagB (rHagB) as well as antibodies to HagB, including the polyclonal antibody (PAb) A7985 and the monoclonal antibody (MAb) HL1858, to inhibit the attachment of P. gingivalis to HCAE cells. As expected, when the attachment of P. gingivalis and the HagB mutant were compared, no statistical significance was observed between the two groups (P ‫؍‬ 0.331), likely due to the expression of the hagB homolog hagC. However, E. coli-HagB adhered significantly better to HCAE cells than did E. coli-pUC9, the control strain. In a competition assay, the presence of purified rHagB decreased bacterial adhesion of P. gingivalis or E. coli-HagB to HCAE cells. The presence of PAb A7985 or MAb HL1858 also significantly decreased attachment of P. gingivalis and E. coli-HagB to host cells. These results indicate that HagB is involved in the adherence of P. gingivalis to human primary endothelial cells.

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Eighty-Kilodalton N-Terminal Moiety of Bordetella pertussis Filamentous Hemagglutinin: Adherence, Immunogenicity, and Protective Role

Cited by 32 publications

References 47 publications

Construction and Characterization of Single-Chain Variable Fragment Antibodies Directed against the Bordetella pertussis Surface Adhesins Filamentous Hemagglutinin and Pertactin

Construction and Characterization of Single-Chain Variable Fragment Antibodies Directed against the Bordetella pertussis Surface Adhesins Filamentous Hemagglutinin and Pertactin

Production of Nontypeable Haemophilus influenzae HtrA by Recombinant Bordetella pertussis with the Use of Filamentous Hemagglutinin as a Carrier

Hemagglutinin B Is Involved in the Adherence of Porphyromonas gingivalis to Human Coronary Artery Endothelial Cells

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