Protective action of ultrasound-guided intraparenchymal transplantation of BMSCs in adriamycin nephropathy rats through the RIPK3/MLKL and NLRP3 pathways

Xia, Chunjuan; Shao, Lishi; Ma, Yiqun; Wang, Xinghong; Ya, Zhang; Cheng, Sheue-yann; Li, Jiaqi; Zhang, Weihu; Zhang, Dongliang; Wang, Jiaping

doi:10.1016/j.acthis.2021.151773

Cited by 2 publications

(2 citation statements)

References 51 publications

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“…The therapeutic effects of FHD on renal function and histopathological changes induced by NS were further evaluated. SCr, BUN, and ALB are three critical indicators widely used to identify kidney function [ 31 ]. As shown in Figures 8(a) – 8(c) , the levels of SCr and BUN were significantly increased in serum of NS rats, and ALB level was decreased in comparison to that in control rats ( P < 0.001).…”

Section: Resultsmentioning

confidence: 99%

Network Pharmacology and In Vivo Experimental Validation to Uncover the Renoprotective Mechanisms of Fangji Huangqi Decoction on Nephrotic Syndrome

Yin

Mao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Fangji Huangqi decoction (FHD) is a traditional Chinese medicine formula that has the potential efficacy for nephrotic syndrome (NS) treatment. This study aims to explore the effects and underlying mechanisms of FHD against NS via network pharmacology and in vivo experiments. Methods. The bioactive compounds and targets of FHD were retrieved from the TCMSP database. NS-related targets were collected from GeneCards and DisGeNET databases. The compound-target and protein-protein interaction networks were constructed by Cytoscape 3.8 and BisoGenet, respectively. GO and KEGG analyses were performed by the DAVID online tool. The interactions between active compounds and hub genes were revealed by molecular docking. An NS rat model was established to validate the renoprotective effects and molecular mechanisms of FHD against NS in vivo. Results. A total of 32 hub genes were predicted to play essential roles in FHD treating NS. Eight main bioactive compounds of FHD had the good affinity with 9 hub targets (CCL2, IL-10, PTGS2, TNF, MAPK1, IL-6, CXCL8, TP53, and VEGFA). The therapeutic effect of FHD on NS was closely involved in the regulation of inflammation and PI3K-Akt pathway. In vivo experiments confirmed the renoprotective effect of FHD on NS, evidenced by reducing the levels of proteinuria, serum creatinine, blood urea nitrogen, and inflammatory factors in NS rats. The PI3K activator 740Y-P weakened the effects of FHD against NS. Furthermore, FHD downregulated the levels of PTGS2, MAPK1, IL-6, and p-Akt in NS rats. Conclusions. FHD alleviates kidney injury and inflammation in NS by targeting PTGS2, MAPK1, IL-6, and PI3K-Akt pathway.

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Section: Resultsmentioning

confidence: 99%

Network Pharmacology and In Vivo Experimental Validation to Uncover the Renoprotective Mechanisms of Fangji Huangqi Decoction on Nephrotic Syndrome

Yin

Mao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Liao et al [ 128 ] found that LIPUS (1.5 MHz; duty cycle, 20%; intensity, 30 mW/cm 2 ; 20 min/d) could promote ECM synthesis, inhibit the inflammatory response, and inhibit NF–kB pathway activation. Xia et al [ 129 ] found that the expression of NLRP3 protein on the BMSC cells of mice treated with ultrasound was significantly reduced, and the secretion of inflammatory factors such as IL–1β and IL–18 was inhibited. This suggests that ultrasound can inhibit the initiation phase of the NLRP3 inflammasome.…”

Section: Inflammation Under the Mechanical Environment In Bone Scaffoldsmentioning

confidence: 99%

Inflammation Responses to Bone Scaffolds under Mechanical Stimuli in Bone Regeneration

Wang

Yuan

Yin

et al. 2023

JFB

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Physical stimuli play an important role in one tissue engineering. Mechanical stimuli, such as ultrasound with cyclic loading, are widely used to promote bone osteogenesis; however, the inflammatory response under physical stimuli has not been well studied. In this paper, the signaling pathways related to inflammatory responses in bone tissue engineering are evaluated, and the application of physical stimulation to promote osteogenesis and its related mechanisms are reviewed in detail; in particular, how physical stimulation alleviates inflammatory responses during transplantation when employing a bone scaffolding strategy is discussed. It is concluded that physical stimulation (e.g., ultrasound and cyclic stress) helps to promote osteogenesis while reducing the inflammatory response. In addition, apart from 2D cell culture, more consideration should be given to the mechanical stimuli applied to 3D scaffolds and the effects of different force moduli while evaluating inflammatory responses. This will facilitate the application of physiotherapy in bone tissue engineering.

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Protective action of ultrasound-guided intraparenchymal transplantation of BMSCs in adriamycin nephropathy rats through the RIPK3/MLKL and NLRP3 pathways

Cited by 2 publications

References 51 publications

Network Pharmacology and In Vivo Experimental Validation to Uncover the Renoprotective Mechanisms of Fangji Huangqi Decoction on Nephrotic Syndrome

Network Pharmacology and In Vivo Experimental Validation to Uncover the Renoprotective Mechanisms of Fangji Huangqi Decoction on Nephrotic Syndrome

Inflammation Responses to Bone Scaffolds under Mechanical Stimuli in Bone Regeneration

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