Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV

Zhu, Faming; Du, Huahua; Miao, Zhiguo; Quan, Haizhi; Xu, Zhenghao

doi:10.1016/j.fsi.2009.02.022

Cited by 54 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These morphological aspects also were been observed by other authors in ultrastructural study for the WSSV (Chou et al, 1995;Durand et al, 1997;Wang et al, 2000;Rajendran et al, 2004;Van Hulten et al, 2001;Afsharnasab, 2006;Escobedo et al, 2008;Zhu et al, 2009).…”

Section: Discussionsupporting

confidence: 83%

Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques

et al. 2012

View full text Add to dashboard Cite

SUMMARY:In this study thirty shrimp samples from commercial marine shrimp (L. vannamei) farms of southern region of Brazil were obtained. Hepatopancreas and shell scrapings fragments collected in these animals were processed by transmission electron microscopy using negative staining (rapid preparation), immunoelectron microscopy and immunocytochemistry (immunolabelling with colloidal gold particles) techniques. On the transmission electron microscopy a great number of white spot virus particles, ovoid or bacilliform-to-ellipsoid, measured 230-290 nm in length and 80-160 nm in diameter with intra-nuclear projections were visualized by the negative staining technique in 27 (90%) out of 30 samples examined. Using immunoelectron microscopy technique, the anti-VP 664 serum agllutinated a large number of particles formed by antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction was styrongly marked by the particles of colloidal gold over the virus. Notably, this is the first report, to our knowledge, describing use of these microscopy techniques to study Brazilian L. vannamei marine shrimp samples; moreover, this methodology also appears to be a viable complementary tool for diagnosing the presence of the white spot virus within shrimp tissues. Importantly, these are the first photoelectron micrographs of the WSSV in Brazil.

show abstract

Section: Discussionsupporting

confidence: 83%

Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques

et al. 2012

View full text Add to dashboard Cite

show abstract

“…In this experiment, the third group acts as a positive control and the fourth group acts as a negative control (without challenge). The mortality was recorded for a 15-day period and the relative percentage survival (RPS) was calculated from the formula as described by Zhu et al [3]. The quantity of PAP-phMGFP that gave the best RPS was chosen for use in the next experiments.…”

Section: Protection Of Shrimp By Pap Gene Immunizationmentioning

confidence: 99%

“…Adaptive immune responses by crustaceans depend on their innate immune response systems [1] such as humoral and cellular immune systems [2]. Some studies have reported that shrimp immune responses cannot be stimulated except from infective agents or derivatives therefrom [3,4].…”

Section: Introductionmentioning

confidence: 99%

Stimulating the immune response of Litopenaeus vannamei using the phagocytosis activating protein (PAP) gene

Khimmakthong

Deachamag

Phongdara

et al. 2011

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Attempts have also been made to develop suitable preventive methods against several shrimp viruses. Some of the research works have proved that the immune system of shrimp could be induced using inactivated pathogens or recombinant proteins against virus [13,31,47,56]. However, variations in results have been seen while using the recombinant proteins from different expression systems [10,46].…”

Section: Introductionmentioning

confidence: 99%

Host and Virus Protein Interaction Studies in Understanding Shrimp Virus Gene Function

Otta

2012

Indian J. Virol.

View full text Add to dashboard Cite

Protein-protein interaction studies have been widely used in several fields to characterize an unknown protein. This in turn helps to find out several pathways to understand a complex mechanism or discover a drug for treatment. Among the methods, yeast two-hybrid has widely been used in human, animal and plant research studies. This aspect of research has also been found useful in understanding the shrimp virus gene function. With respect to White spot syndrome virus, interaction studies have been applied to elucidate virus structure, understand the mode of entry of the virus, mechanism of virus replication and also to discover some of the host anti-viral proteins. Interaction studies on other shrimp viruses are scanty and only few reports available on Yellow head virus and Taura syndrome virus. All these findings are still in preliminary stage and lot more studies are necessary to have the clear picture. Protein interaction research on other shrimp viruses are still lacking. Considering all these, it appears that this field of research has a wide scope to understand the virulence mechanism of shrimp viruses where very little information is available till date.

show abstract

Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV

Cited by 54 publications

References 38 publications

Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques

Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques

Stimulating the immune response of Litopenaeus vannamei using the phagocytosis activating protein (PAP) gene

Host and Virus Protein Interaction Studies in Understanding Shrimp Virus Gene Function

Contact Info

Product

Resources

About