Protection of Procambarus clarkii against white spot syndrome virus using recombinant oral vaccine expressed in Pichia pastoris

Effects of oral recombinant VP28 expressed in silkworm (Bombyx mori) pupa on immune response and disease resistance of Procambarus clarkii

“…But the application of recombinant proteins to prevent diseases in shrimp is still attractive and promising in the future. In our laboratory, several heterologous systems have produced recombinant versions of VP28 protein Jha et al 2007;Xu et al 2006), and research revealed that the produced recombinant VP28 protein in eukaryotic expression system (BmN cells) gave much better protection than that in prokaryotic expression system (E. coli; Du et al 2006).…”

Histological alterations and immune response in the crayfish Procambarus clarkii given rVP28-incorporated diets

“…These data tend to indicate that persistent exposure to viral protein may be required for continued protection. Especially, recombinant VP28 protein can enhance significantly immune activity and disease resistance of shrimp species [4,6,10,11]. The baculovirus expression vector system (BEVS) and silkworm as a bioreactor is commonly used to produce recombinant proteins for animal medicines, when production costs restrain their use, and the produced protein could be used directly without any purification [12,13].…”

“…Recent research indicated that apoptosis is proposed as the main anti-viral non-specific defense mechanism in crustaceans [5,6] and some immunostimulants can modulate host cell apoptosis in a dose-dependent manner [7e9]. Now, several heterologous systems have been used to produce recombinant versions of VP28 protein [4,6,10,11]. The domesticated silkworm, Bombyx mori, pupae are of rich nutrient and low toxicity, and traditionally used as animal feed, food material and traditional medicine in some countries, such as China, Japan and India.…”

Effectiveness of Natural Herbal Oil Formulation against White Spot Syndrome Virus in Penaeus vannamei

“…The cumulative mortality in infetcted shrimp reaches up to 100% in 4-7 days in lab and 10-14 days in the ponds [3][4][5][6]. The intensity of infection and severity depends on the viral load.…”