Protection of Procambarus clarkii against white spot syndrome virus using recombinant subunit injection vaccine expressed in Pichia pastoris

Abstract

The potentiality of injection vaccine against white spot syndrome virus (WSSV) in crayfish Procambarus clarkii was investigated. WSSV envelope proteins VP19 and VP28 were expressed in yeast Pichia pastoris GS115. The purified recombinant proteins (2 µg/g of crayfish) were injected intramuscularly, and the same dose injected as a booster shot on fifth day after vaccination. The vaccinated crayfish were divided into two even groups and later challenged orally by WSSV-infected dead crayfish muscle (2 g/individual) on the third and 21st days after the booster shot. The relative percent survival (RPS) in the third-day group was the highest in VP28 (91%), followed by VP19 + VP28 (84%), and VP19 (45%). The RPS for the 21st-day group was the highest in VP28 (78%), followed by VP19 + VP28 (76%), and VP19 (17%). Development of vaccine by using recombinant proteins VP19 and VP28 expressed in yeast is feasible.

Comparative Efficacy of dsRNA VP24, VP26, RR1 and WSV477 Gene against WSSV Infection in Penaeus monodon

“…Hence understanding the immune system of shrimps in much detailed manner is the need of the hour to establish greater and efficient methods to control the infectious diseases and also to ensure the long-term viability of the shrimp aquaculture practices. To overcome the impacts of WSSV various therapeutic approaches are followed, that includes manipulating environmental conditions, administrating various immune modulators (Chang et al, 2003;Balasubramanian et al, 2007), vaccination (Huang et al, 2005;Jha et al, 2006), and RNA interference (RNAi) strategies (Robalino et al, 2004;Xu et al, 2007). RNAi, an evolutionary conserved natural mechanism that was initially studied in the nematode, Caenorhabditis elegans (Fire et al1998).…”

Effectiveness of Natural Herbal Oil Formulation against White Spot Syndrome Virus in Penaeus vannamei

“…The cumulative mortality in infetcted shrimp reaches up to 100% in 4-7 days in lab and 10-14 days in the ponds [3][4][5][6]. The intensity of infection and severity depends on the viral load.…”