Protection of LDL from oxidation by olive oil polyphenols is associated with a downregulation of CD40-ligand expression and its downstream products in vivo in humans

Castañer, Olga; Covas, Marı́a-Isabel; Khymenets, Olha; Nyyssönen, Kristiina; Konstantinidou, Valentini; Zunft, H.‐J.; Torre, Rafael de la; Muñoz-Aguayo, Daniel; Vila, Joan; Fitó, Montserrat

doi:10.3945/ajcn.111.029207

Cited by 115 publications

(108 citation statements)

References 30 publications

(32 reference statements)

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“…Apart from the ability to inhibit low-density lipoprotein oxidation and the suppression of circulating monocyte chemoattractant protein-1, consumption of olive oil with a high polyphenol content for 3 weeks resulted in the reduced expression of proatherogenic and proinflammatory genes in the blood monocytes of healthy subjects (Castaner et al, 2012). Besides direct radical scavenging, this indicates that anti-inflammatory properties of polyphenols may contribute to their antioxidant effect.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Nowak

Zasowska-Nowak

Białasiewicz

et al. 2015

Pharmaceutical Biology

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Context: Polymorphonuclear leukocytes (PMNs) produce oxidants, contributing to systemic oxidative stress. Diets rich in plant polyphenols seem to decrease the risk of oxidative stressinduced disorders including cardiovascular disease. Objective: The objective of this study was to examine the in vitro effect of each of the 14 polyphenols on PMNs chemotaxis, intracellular calcium response, oxidants production. Materials and methods: Blood samples and PMNs suspensions were obtained from 60 healthy non-smoking donors and incubated with a selected polyphenol (0.5-10 mM) or a control solvent. We assessed resting and fMLP-dependent changes of intracellular calcium concentration ([Ca 2+ ] i ) in PMNs with the Fura-2AM method and measured fMLP-induced luminol enhanced whole blood chemiluminescence (fMLP-LBCL). Polyphenol chemoattractant activity for PMNs was tested with Boyden chambers. Results: Polyphenols had no effect on resting [Ca 2+ ] i . Unaffected by other compounds, fMLPdependent increase of [Ca 2+ ] i was inhibited by quercetin and catechol (5 mM) by 32 ± 14 and 12 ± 10% (p50.04), respectively. Seven of the 14 tested substances (5 mM) influenced fMLP-LBCL by decreasing it. Catechol, quercetin, and gallic acid acted most potently reducing fMLP-LBCL by 49 ± 5, 42 ± 15, and 28 ± 18% (p50.05), respectively. 3,4-Dihydroxyhydrocinnamic, 3,4-dihydroxyphenylacetic, 4-hydroxybenzoic acid, and catechin (5 mM) revealed distinct (p50.02) chemoattractant activity with a chemotactic index of 1.9 ± 0.8, 1.8 ± 0.7, 1.6 ± 0.6, 1.4 ± 0.2, respectively. Conclusion and discussion: Catechol, quercetin, and gallic acid at concentrations commensurate in human plasma strongly suppressed the oxidative response of PMNs. Regarding quercetin and catechol, this could result from an inhibition of [Ca 2+ ] i response.

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Section: Introductionmentioning

confidence: 99%

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Nowak

Zasowska-Nowak

Białasiewicz

et al. 2015

Pharmaceutical Biology

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“…The effects of LACHI MIX HT were examined on oxidative stress levels, chloride and potassium channel conductances and excitability of the sarcolemma, calcium homeostasis and contractile function, muscle atrophy, and muscle force, all of which are sensitive to the activity of ROS. The effects of LACHI MIX HT were compared with those of purified HT, already proposed in different therapies, i.e., anticancer or cardioprotective (Granados-Principal et al 2010;Warleta et al 2011;Castañer et al 2012). …”

Section: Introductionmentioning

confidence: 99%

An olive oil-derived antioxidant mixture ameliorates the age-related decline of skeletal muscle function

Pierno

Tricarico

Liantonio

et al. 2013

AGE

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Age-related skeletal muscle decline is characterized by the modification of sarcolemma ion channels important to sustain fiber excitability and to prevent metabolic dysfunction. Also, calcium homeostasis and contractile function are impaired. In the aim to understand whether these modifications are related to oxidative damage and can be reverted by antioxidant treatment, we examined the effects of in vivo treatment with an waste water polyphenolic mixture (LACHI MIX HT) supplied by LACHIFARMA S.r.l. Italy containing hydroxytirosol (HT), gallic acid, and homovanillic acid on the skeletal muscles of 27-month-old rats. After 6-week treatment, we found an improvement of chloride ClC-1 channel conductance, pivotal for membrane electrical stability, and of ATP-dependent potassium channel activity, important in coupling excitability with fiber metabolism. Both of them were analyzed using electrophysiological techniques. The treatment also restored the resting cytosolic calcium concentration, the sarcoplasmic reticulum calcium release, and the mechanical threshold for contraction, an index of excitation-contraction coupling mechanism. Muscle weight and blood creatine kinase levels were preserved in LACHI MIX HT-treated aged rats. The antioxidant activity of LACHI MIX HT was confirmed by the reduction of malondialdehyde levels in the brain of the LACHI MIX HT-treated aged rats. In comparison, the administration of purified HT was less effective on all the parameters studied. Although muscle function was not completely recovered, the present study provides evidence of the beneficial effects of LACHI MIX HT, a natural compound, to ameliorate skeletal muscle functional decline due to aging-associated oxidative stress.

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“…Olive oil and red wine antioxidant polyphenols inhibit endothelial activation which is one of the antiatherogenic properties of Mediterranean diet polyphenols [19]. In addition to reducing LDL oxidation, the dietary intake of polyphenol-rich olive oil is capable of reducing the expression of CD40L gene, its downstream products, and related genes those are actively involved in atherogenic and inflammatory processes in humans [6].…”

Section: Resultsmentioning

confidence: 99%

“…Polyphenols display protective action against LDL oxidation and potentially against CVD like atherogenesis. Polyphenols could exert health benefits not only by scavenging free radicals but also by modulating gene expression [6]. …”

Section: Mode Of Action Of Polyphenol By Scavange Rosmentioning

confidence: 99%

Cardio-Protecting Effect of Natural Bioactive Compound (Polyphenol) by Inhibiting LDL Oxidation with the Scavenging of Reactive Oxygen Species (ROS)

Manna¹,

Maiti²

2016

J Clin Exp Cardiolog

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Reduction in plasma low-density lipoprotein is a fundamental treatment for the prevention of acute coronary syndromes (ACS). Oxidization of low-density lipoprotein (LDL) particles leads to formation of atherosclerotic lesions and increased risk of cardiovascular disease (CVD) via a complex cascade of biochemical events occurring mostly within the arterial wall. The major advantage of drug those are treating with hypertension, hyperglycemia and hyperlipidemia to reduce the CVD morbidity and mortality are well established. However, the epidemiological evidences support that ingestion of certain foods in regular diet chart results to a reduction in of myocardial infarction markers and other CVD. Many reviews supported that the dietary antioxidants pertained to LDL oxidation and to vascular endothelial dysfunction. Polyphenol consumption is beneficial to maintain the plasma LDL level. This short commentary emphasising on health aspect depending on the published literature, which may provide some guidance for researchers in further investigations of cardiovascular health agents.

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Protection of LDL from oxidation by olive oil polyphenols is associated with a downregulation of CD40-ligand expression and its downstream products in vivo in humans

Cited by 115 publications

References 30 publications

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

An olive oil-derived antioxidant mixture ameliorates the age-related decline of skeletal muscle function

Cardio-Protecting Effect of Natural Bioactive Compound (Polyphenol) by Inhibiting LDL Oxidation with the Scavenging of Reactive Oxygen Species (ROS)

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