Protection against radiation-induced oxidative damage by an ethanolic extract ofNigella sativaL.

Abstract

The results obtained from the different experimental systems suggest the radioprotective ability of EE-NS involving prevention of radiation-induced oxidative damage.

Chlorisondamine, a sympathetic ganglionic blocker, moderates the effects of whole-body irradiation (WBI) on early host defense to a live bacterial challenge

“…This contrasts reports of increases in splenic ROS levels 24 h after exposure to 2 Gy [66]. Thymocytes isolated from mice 5-7 days after exposure to 1.5-3 Gy also had increased levels of ROS.…”

Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review

“…Oral administration of N. sativa oil (1 ml/kg/day for 5 days/week) prior to irradiation considerably attenuated the irradiation-related biochemical, immunological, and pathological effects [41]. Along the same lines, Rastogi and colleagues demonstrated that an ex vivo treatment of mouse splenic lymphocytes with an ethanolic extract of N. sativa 1 h prior to irradiation prevented that the formation of lipid peroxides and intracellular ROS; factors that mediate radiation-induced DNA damage and apoptosis [42]. Furthermore, oral administration of an ethanolic extract of N. sativa (100 mg/kg/day for 5 days) protected irradiated Swiss albino mice against oxidative injury to the spleen and liver tissues in as measured by the activity of anti-oxidant enzymes and lipid peroxidation [42].…”

“…Along the same lines, Rastogi and colleagues demonstrated that an ex vivo treatment of mouse splenic lymphocytes with an ethanolic extract of N. sativa 1 h prior to irradiation prevented that the formation of lipid peroxides and intracellular ROS; factors that mediate radiation-induced DNA damage and apoptosis [42]. Furthermore, oral administration of an ethanolic extract of N. sativa (100 mg/kg/day for 5 days) protected irradiated Swiss albino mice against oxidative injury to the spleen and liver tissues in as measured by the activity of anti-oxidant enzymes and lipid peroxidation [42]. These radio-protective effects were attributed to the radical scavenging potential of N. sativa [42].…”

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“…Moreover, radiation-induced DNA damage was prevented in splenocytes pre-treated with NS ethanolic extract 52 . The liver tissue total oxidant status, lipid hydroperoxide level, and oxidative stress index were increased in the irradiated rats compared to the control rats, while total antioxidant status and sulfhydryl (-SH) levels were significantly declined.…”

“…It caused an increase in the activities of SOD, catalase (CAT) and glutathione peroxidase (GPX) 51 . Also, NS had radio-protective effects against radiation-related oxidative stress and NS treatment meaningfully antagonized the effects of radiation; therefore, NS may be a beneficial agent in protection against ionizing radiationrelated tissue injury 52 . Ex-vivo treatment of mouse splenic lymphocytes with an ethanolic extract of NS before irradiation showed significant inhibition of the formation of lipid-peroxides and intracellular reactive oxygen species (ROS), which correlated with radiation-induced apoptosis.…”