Protection against leptospirosis conferred by Mycobacterium bovis BCG expressing antigens from Leptospira interrogans

Engineering a new vaccine platform for heterologous antigen delivery in live-attenuated Mycobacterium tuberculosis

“…1 ). The fact that DTP and other antigens have been efficiently expressed in BCG using comparable tools [48] , [49] , [50] , [51] reinforces the notion that heterologous expression in M. tuberculosis is not trivial.…”

Landscape of immunobiological properties of BCG: from vaccine vector to immunotherapy

“…the four portions of this chimera were individually cloned into the BcG to assess which one would be involved in the protection against leptospirosis. the results demonstrated 100% protection against leptospirosis and all rBcG strains significantly prevented renal colonization by Leptospira bacteria (Dorneles et al, 2020).…”

“…live vaccines with recombinant vectors are potential alternatives to increase the protection range and sustained stimulation of the immune system. the use of BcG as a vector for recombinant expression of heterologous antigens has been tested for several pathogens (Dorneles et al, 2020;Goulart et al, 2017;Soto et al, 2018). Goulart et al (2017) developed rBcG strains that are capable of inducing an effective immune response and protecting against sepsis caused by Streptococcus pneumoniae.…”

The Diverse Applications of Recombinant BCG-Based Vaccines to Target Infectious Diseases Other Than Tuberculosis: An Overview

“…A recent study by Dorneles et al used various combinations of Leptospira antigens to target the zoonotic bacterial disease leptospirosis ( Dorneles et al, 2020 ). Upon subcutaneous inoculation of hamsters with four rBCG constructs, each encoding a distinct portion of a recombinant chimera made of various leptospiral antigens, all constructs provided full protection against leptospirosis challenge through the induction of cellular immune responses, in contrast to the BCG controls which had no protection ( Dorneles et al, 2020 ). However, rBCG anti-bacterial vaccines have also been shown to elicit strong humoral responses.…”