Protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying E7 antigen at its surface

Ribelles, Pedro; Benbouziane, Bouasria; Langella, Philippe; Súarez, Juan E.; Bermúdez‐Humarán, Luis G.; Riazi, Ali

doi:10.1007/s00253-012-4575-1

Cited by 47 publications

(30 citation statements)

References 24 publications

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“…In another field of application, Lc-Lys CWBD appears already as a promising candidate to anchor noncovalent proteins at the bacterial surface as previously shown to obtain vaccines or probiotic bacteria (39).…”

Section: Discussionmentioning

confidence: 98%

A Novel Type of Peptidoglycan-binding Domain Highly Specific for Amidated d-Asp Cross-bridge, Identified in Lactobacillus casei Bacteriophage Endolysins

Regulski

Courtin

Kulakauskas

et al. 2013

Journal of Biological Chemistry

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Background: Peptidoglycan hydrolases, including bacterial autolysins and bacteriophage endolysins, contain generally a cell wall-binding domain (CWBD), responsible for their high affinity and specificity toward target cell walls. Results: Two Lactobacillus casei endolysins lyse only bacterial cells with a D-Asn cross-bridge in their peptidoglycan. Conclusion: The CWBD of these two endolysins recognizes specifically peptidoglycan with a D-Asn cross-bridge. Significance: This CWBD is a novel type of peptidoglycan-binding domain.

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Section: Discussionmentioning

confidence: 98%

A Novel Type of Peptidoglycan-binding Domain Highly Specific for Amidated d-Asp Cross-bridge, Identified in Lactobacillus casei Bacteriophage Endolysins

Regulski

Courtin

Kulakauskas

et al. 2013

Journal of Biological Chemistry

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“…LysA2, the L. casei bacteriophage A2 lysine, consists of an N-terminal catalytic domain and a C-terminal cell wall binding domain (CBD), which was previously shown to bind with high affinity to L. lactis (23). By virtue of this property, LysA2 CBD was here used to efficiently immobilize Hsa and PadA on the surface of L. lactis, which served as an antigen-presenting system.…”

Section: Resultsmentioning

confidence: 99%

“…We used for the immunization a recently developed novel display system that allows highly efficient immobilization of antigens on the surface of lactic acid bacteria, such as L. lactis (23). This system is based on non-genetically modified L. lactis used as a vehicle for antigens bound on the surface by means of a highaffinity cell-binding domain (CBD) from L. casei A2 phage lysine LysA2 (24).…”

Section: Discussionmentioning

confidence: 99%

Antibodies Targeting Hsa and PadA Prevent Platelet Aggregation and Protect Rats against Experimental Endocarditis Induced by Streptococcus gordonii

et al. 2016

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Streptococcus gordonii and related species of oral viridans group streptococci (VGS) are common etiological agents of infective endocarditis (IE). We explored vaccination as a strategy to prevent VGS-IE, using a novel antigen-presenting system based on non-genetically modified Lactococcus lactis displaying vaccinogens on its surface. Hsa and PadA are surface-located S. gordonii proteins implicated in platelet adhesion and aggregation, which are key steps in the pathogenesis of IE. This function makes them ideal targets for vaccination against VGS-IE. In the present study, we report the use of nonliving L. lactis displaying at its surface the N-terminal region of Hsa or PadA by means of the cell wall binding domain of Lactobacillus casei A2 phage lysine LysA2 (Hsa-LysA2 and PadA-LysA2, respectively) and investigation of their ability to elicit antibodies in rats and to protect them from S. gordonii experimental IE. Immunized and control animals with catheter-induced sterile aortic valve vegetations were inoculated with 10 6 CFU of S. gordonii. The presence of IE was evaluated 24 h later. Immunization of rats with L. lactis HsaLysA2, L. lactis PadA-LysA2, or both protected 6/11 (55%), 6/11 (55%), and 11/12 (91%) animals, respectively, from S. gordonii IE (P < 0.05 versus controls). Protection correlated with the induction of high levels of functional antibodies against both Hsa and PadA that delayed or totally inhibited platelet aggregation by S. gordonii. These results support the value of L. lactis as a system for antigen delivery and of Hsa and PadA as promising candidates for a vaccine against VGS-IE.

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“…Common vaginal lactobacilli exerted cytotoxic effects on cervical tumour cells independently of pH and lactate [96] . L. casei displaying E7 antigen at its surface protected mice against human papillomavirus type 16-induced tumours [97] . As regard hepatocarcinoma, the administration of probiotic fermented milk containing L. rhamnosus GG and, L. casei strain Shirota with chlorophyllin reduced liver pre-carcinogenic events in rat AFB1 induced liver carcinogenesis.…”

Section: Nutrtion and Breast Cancermentioning

confidence: 99%

Modification in the diet can induce beneficial effects against breast cancer

Aragón

Perdigón

LeBlanc

2014

WJCO

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Protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying E7 antigen at its surface

Cited by 47 publications

References 24 publications

A Novel Type of Peptidoglycan-binding Domain Highly Specific for Amidated d-Asp Cross-bridge, Identified in Lactobacillus casei Bacteriophage Endolysins

A Novel Type of Peptidoglycan-binding Domain Highly Specific for Amidated d-Asp Cross-bridge, Identified in Lactobacillus casei Bacteriophage Endolysins

Antibodies Targeting Hsa and PadA Prevent Platelet Aggregation and Protect Rats against Experimental Endocarditis Induced by Streptococcus gordonii

Modification in the diet can induce beneficial effects against breast cancer

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