Protection against Gluten-mediated Tight Junction Injury with a Novel Lignite Extract Supplement

Abstract

Background: Tight junctions are found in epithelial cells and function as selective gatekeepers to regulate absorption. PT-gliadin is the gluten protein segment that is known to impair the functioning of tight junctions. This study aimed to examine the effects of a lignite extract dietary supplement (RESTORE) on tight junction function in small intestine (IEC-6) and colon (Caco-2) epithelial cells. The study also evaluated the biologic safety of the same supplement as established by the rates of apoptosis in the intestinal and proximal renal tubule cell cultures treated with the supplement.

Protective Effects of Lignite Extract Supplement on Intestinal Barrier Function in Glyphosate-Mediated Tight Junction Injury

“…Transepithelial electrical resistance (TEER) is widely accepted as an indicator of tight junction permeability [17][18][19][20]. TEER was measured in both IEC-6 and Caco-2 cells seeded in 24-well transwell plates (1-micron pore size, Becton Dickinson).…”

Agriculture, the Anthropocene and human health

“…Work by leading autism researcher Dr. Zach Bush and his team, in addition to supporting the above scenario, shows that another major deleterious impact of glyphosate is interference in healthy epigenetic function in the gut: the switching on and off of key genes in our gut microbes and their regulation of healthy bodily and immune function 18,19 …”