Protection against experimental Aeromonas salmonicida infection in carp by oral immunisation with bacterial antigen entrapped liposomes

Irie, Takahiro; Watarai, Shinobu; Iwasaki, Tadashi; Kodama, Hiroshi

doi:10.1016/j.fsi.2004.07.006

Cited by 60 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As far as we know, this study is the first attempt to co-encapsulate two model immunostimulants specifically designed to target fish APCs in nanosized liposomes. To date, the unique attempt to vaccinate fish using liposomes was done by Irie et al , who explored the use of microsized liposomes containing A. salmonicida total extracts in carp [22]. Recently, Fredriksen et al have also shown that a combination of poly(lactic- co -glycolic acid) microparticles loaded with β-glucan and human γ-globulins were able to target head kidney macrophages inducing an adaptive in vivo immune response in salmon [41].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

et al. 2013

View full text Add to dashboard Cite

Development of novel systems of vaccine delivery is a growing demand of the aquaculture industry. Nano- and micro- encapsulation systems are promising tools to achieve efficient vaccines against orphan vaccine fish diseases. In this context, the use of liposomal based-nanocarriers has been poorly explored in fish; although liposomal nanocarriers have successfully been used in other species. Here, we report a new ∼125 nm-in-diameter unilamellar liposome-encapsulated immunostimulant cocktail containing crude lipopolysaccharide (LPS) from E. coli and polyinosinic:polycytidylic acid [poly (I:C)], a synthetic analog of dsRNA virus, aiming to be used as a non-specific vaccine nanocarrier in different fish species. This liposomal carrier showed high encapsulation efficiencies and low toxicity not only in vitro using three different cellular models but also in vivo using zebrafish embryos and larvae. We showed that such liposomal LPS-dsRNA cocktail is able to enter into contact with zebrafish hepatocytes (ZFL cell line) and trout macrophage plasma membranes, being preferentially internalized through caveolae-dependent endocytosis, although clathrin-mediated endocytosis in ZFL cells and macropinocytocis in macrophages also contribute to liposome uptake. Importantly, we also demonstrated that this liposomal LPS-dsRNA cocktail elicits a specific pro-inflammatory and anti-viral response in both zebrafish hepatocytes and trout macrophages. The design of a unique delivery system with the ability to stimulate two potent innate immunity pathways virtually present in all fish species represents a completely new approach in fish health.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Some of these studies have suggested that microencapsulated vaccines significantly enhance the protection and immune response in various fish species [19]–[22]. Thus far, however, no one has demonstrated the ability to simultaneously control the encapsulation of several immunostimulants in unilamellar, biocompatible liposomes.…”

Section: Introductionmentioning

confidence: 99%

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Thus, considering the mortality of the fish with natural low immune potency, the resulted data of the challenge test are meaningful to evaluate successful oral immunization with the A. hydrophila liposome vaccine. Irie et al (2005) also reported that oral administration of liposomes entrapping antigens of atypical A. salmonicida induced immunity in carp. Their liposomes differed from the liposomes prepared by us in having a different molar ratio of phospholipids and a different antigen-containing compartment.…”

Section: Discussionmentioning

confidence: 94%

Oral Immunization of Common Carp with a Liposome Vaccine Containing Aeromonas hydrophila Antigen

信哉¹,

哲郎²,

照雄³

2006

Fish Pathol.

View full text Add to dashboard Cite

“…Hal ini diduga karena adanya kerusakan antigen dari vaksin pada saat melewati saluran pencernaan, dan kesempatan setiap ikan memakan pakan yang mangandung vaksin tidak sama jumlahnya. Terdapat beberapa pendekatan yang dapat diambil dari kasus ini adalah dengan menggunakan bantuan liposom atau alginat untuk melindungi antigen dari tingkat keasaman saluran pencernaan, metode ini lebih dikenal dengan vaksin biofilm (Azad et al, 2000;Ire et al, 2005).…”

Section: Penggunaan Vaksin Bivalen a Hydrophila Dan M Fortuitum Melunclassified

EFEKTIVITAS VAKSIN BIVALEN Aeromonas hydrophila DAN Mycobacterium fortuitum UNTUK PENCEGAHAN INFEKSI PENYAKIT PADA IKAN GURAMI (Osphronemus goramy)

Sugiani

Aryati

Mufidah

et al. 2015

Jurnal Riset Akuakultur

View full text Add to dashboard Cite

ABSTRAKVaksinasi merupakan salah satu cara terbaik untuk pencegahan terhadap penyakit ikan. Aplikasi pemberian vaksin untuk skala massal oleh pembudidaya ikan masih menjadi kendala. Tujuan dari penelitian ini adalah untuk menguji efektivitas dan respons peningkatan kekebalan tubuh ikan terhadap penggunaan vaksin bivalen anti A. hydrophila dan M. fortuitum pada penanggulangan kejadian ko-infeksi A. hydrophila dan M. fortuitum pada ikan gurami. Perlakuan vaksinasi pada ikan gurami dilakukan selama empat minggu pemeliharan menggunakan tiga metode yang berbeda yaitu melalui injeksi, perendaman, dan oral, setiap perlakuan diulang sebanyak tiga kali dan diuji tantang selama empat minggu dengan ko-infeksi dari A. hydrophila dan M. fortuitum. Nilai RPS (relative percent survival) terbaik setelah uji tantang untuk penggunaan vaksin bivalen melalui perendaman pada benih ikan gurami adalah sebesar 26,4% lebih rendah dibandingkan dengan metode injeksi (56,33%). Vaksin bivalen kurang efektif jika diberikan melalui oral dengan nilai RPS 13,6%. Dosis vaksin bivalen terbaik untuk perlakuan vaksinasi melalui injeksi, perendaman, dan oral adalah campuran antara bakteri A. hydrophila 10 PENDAHULUANVaksinasi ikan untuk melawan berbagai infeksi bakteri patogen secara serempak dapat dilakukan dengan menggunakan vaksin bivalen (vaksin yang mengandung dua jenis patogen) atau polivalen (vaksin yang mengandung beberapa jenis patogen). Strategi vaksinasi yang ideal perlu memenuhi beberapa kriteria berikut ini, penyakit spesifik yang akan dipapar, jenis vaksin, metode vaksinasi, pemilihan waktu vaksinasi, dan perlakuan vaksinasi ulang (booster). Vaksin polivalen harus mampu melindungi dari semua serotipe dari tiap patogen penyebab penyakit tertentu. Namun, kemungkinan adanya kompetisi antigen spesifik yang mungkin terjadi terutama ketika vaksin diaplikasikan melalui injeksi perlu diperhatikan (Toranzo et al., 2009).

show abstract

Protection against experimental Aeromonas salmonicida infection in carp by oral immunisation with bacterial antigen entrapped liposomes

Cited by 60 publications

References 22 publications

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

Oral Immunization of Common Carp with a Liposome Vaccine Containing Aeromonas hydrophila Antigen

EFEKTIVITAS VAKSIN BIVALEN Aeromonas hydrophila DAN Mycobacterium fortuitum UNTUK PENCEGAHAN INFEKSI PENYAKIT PADA IKAN GURAMI (Osphronemus goramy)

Contact Info

Product

Resources

About