Protection against dextran sulfate sodium-induced colitis by microspheres of ellagic acid in rats

Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn's disease

“…Our results are in agreement with previous reports from Ogawa et al [15] and Singh et al [16] with microspheres of ellagic and an ellagic acid rich fraction of Punica granatum extract in dextran sulphate sodium-induced colitis. In a similar way, there has also been described the protective effect of ellagic acid on gastric damage induced in ischemic rat stomachs following ammonia or reperfusion [26].…”

“…Previous reports, have documented that microspheres of ellagic acid [15] and a Punica granatum extract in addition to its ellagic acid rich fraction [16] decreased the degree of inflammation associated with experimental dextran sulphate sodium (DSS)-induced colitis. Intra-colonic administration of trinitrobenzene sulfonic acid (TNBS) is one of standardized methods to produce an experimental model of IBD, which closely mimic the clinical and morphological features of IBD in particular Crohn's disease [17].…”

The Use of Pomegranate (Punica granatum L.) Phenolic Compounds as Potential Natural Prevention Against IBDs

“…Lipid peroxidation in the colonic mucosa, determined by the thiobarbituric acid-reactive substance (TBARS) assay, was significantly decreased by the encapsulated EA treatment, but was not affected by free EA. Similar effects were observed after SOD administration, supporting the idea that EA prevents the colitis development by radical scavenging or other anti-oxidative actions (Ogawa et al, 2002). EA was orally administrated to rats at both doses (10 and 20 mg/kg bw) 48, 24 and 1h before TNBS intra-colonic administration and 24h after.…”

“…Among the in vivo studies on the effects of pomegranate PCs on intestinal inflammation related to IBDs, four studies conducted on murine models were reported in the literature: three with rats (Larrosa et al, 2010;Ogawa et al, 2002;Rosillo et al, 2011) and one with mice (Singh et al, 2009). Experimental models of IBD were induced by a daily oral administration of dextran sulphate sodium (DSS) at 2-5% (v/v) in drinking water or by intra-colonic administration of trinitrobenzene sulfonic acid (TNBS).…”

“…To evaluate the prophylactic effects of EA in the treatment of IBDs (Ogawa et al, 2002), EA was administered to rats, as such or in an encapsulated form. The microsphere capsules allowed EA to reach the terminal ileum and colon where microcapsules dissolved.…”