Protected effect of Esenbeckia leiocarpa upon the inflammatory response induced by carrageenan in a murine air pouch model

Liz, Rafael de; Pereira, Danielle Fontana; Horst, Heros; Dalmarco, Eduardo Monguilhott; Dalmarco, Juliana Bastos; Simionatto, Edésio Luiz; Pizzolatti, Moacir Geraldo; Girard, Denis; Fröde, Tânia Sílvia

doi:10.1016/j.intimp.2011.08.009

Cited by 23 publications

(23 citation statements)

References 26 publications

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“…From our results, it is clear that either CHE or Alk showed agonistic activities for human PMNs. This is consistent with a previous study indicating that E. Leiocarpa exerted an important anti-inflammatory effect in vivo in neutrophils, since a decrease of myeloperoxidase was noted [6], an enzyme considered as an important marker of PMN activation [31]. In general, for all tested functions, PMNs responded to both CHE and Alk in a similar fashion, except for ROS production; CHE inhibited intracellular ROS production but increased the extracellular O 2 - production, whereas Alk (moderately) increased the former and also increased O 2 - production, but not significantly.…”

Section: Discussionsupporting

confidence: 94%

Activation of human neutrophils by Esenbeckia leiocarpa: comparison between the crude hydroalcoholic extract (CHE) and an alkaloid (Alk) fraction

Liz

Horst

Pizzolatti

et al. 2012

Journal of Inflammation

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Esenbeckia leiocarpa, a wide spread native Brazilian tree, was reported recently to possess anti-inflammatory effects in vivo, but the mechanisms involved are still not fully understood and its role in neutrophils is poorly documented. The aim of this study was to compare the effects of a crude hydroalcoholic extract (CHE) and an alkaloid-enriched (Alk) fraction obtained from Esenbeckia leiocarpa bark on human neutrophils by investigating the effect of each fraction alone or in a mixture with classical neutrophil agonists. CHE inhibited intracellular reactive oxygen species (ROS) production but increased the extracellular superoxide (O2-) production, while Alk increased the former and also slightly increased O2- production. We found that CHE and Alk also induced phagocytosis accompanied by Syk activation, adhesion and degranulation. However, neither CHE nor Alk potentiated the effect of classical neutrophil agonists, namely the cytokines GM-CSF for phagocytosis and TNF-α for adhesion or N-formyl-methionyl-leucyl-phenylalanine (fMLP) for degranulation. In addition, based on catalase treatment, CHE and Alk induced neutrophil apoptosis by a hydrogen peroxide (H2O2)-dependent mechanism. Since the elimination of apoptotic neutrophils by professional phagocytes is important for the resolution of inflammation, the ability of CHE and Alk to induce neutrophil apoptosis has to be considered as one possible mechanism associated with the anti-inflammatory activity of these fractions previously reported in vivo.

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Section: Discussionsupporting

confidence: 94%

Activation of human neutrophils by Esenbeckia leiocarpa: comparison between the crude hydroalcoholic extract (CHE) and an alkaloid (Alk) fraction

Liz

Horst

Pizzolatti

et al. 2012

Journal of Inflammation

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“…In addition, the incorporation of phytosterols in erythrocyte membranes accelerates the breakdown of these cells and increases the bilirubin load to the liver 21 . Phytosterols can also increase the risk of sepsis by altering the migratory and phagocytic function of neutrophils as shown in animal models 21 , 24 , 25 …”

Section: What Is Different Between Ivfes?mentioning

confidence: 99%

Intravenous Fat Emulsion Formulations for the Adult and Pediatric Patient

et al. 2016

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Intravenous lipid emulsions (IVLE) provide essential fatty acids (FA) and are a dense source of energy in parenteral nutrition (PN). Parenterally administered lipid was introduced in the 17th century but plagued with side effects. The formulation of lipid emulsions later on made it a relatively safe component for administration to patients. Many ingredients are common to all IVLE, yet the oil source(s) and its (their) percentage(s) makes them different from each other. The oil used dictates how IVLE are metabolized and cleared from the body. The FA present in each type of oil provide unique beneficial and detrimental properties. This review provides an overview of IVLE and discuss factors that would help clinicians choose the optimal product for their patients. Elucidating the characteristics of each oil source over time has resulted in an evolution of the different formulations currently available. Emulsions have gone from being solely made with soybean oil, to being combined with medium-chain triglycerides (i.e., coconut oil), olive oil, and more recently, fish oil. Unfortunately, the lipid, among other constituents in PN formulations, has been associated with the development of liver disease. Lipid-sparing or lipid-reduction strategies have therefore been proposed to avoid these complications. The ideal IVLE would reverse or prevent essential FA deficiency without leading to complications, while simultaneously providing energy to facilitate normal growth and development. Modifications in their ingredients, formulation, and dosing have made IVLE a relatively safe component alone or when added to PN formulations. The ideal emulsion, however, has yet to be developed.

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“…Pretreatment with MEOg, however, significantly reduced TNF-α level. In the pathogenesis of rheumatoid arthritis, TNF-α is known to be an important mediator that plays a part in regulating leuckocyte migration in the acute inflammatory phase; it also activates neutrophils by promoting extravasation to the lungs, liver, gut and other organs; and has a role in extravasated neutrophil damage tissues by releasing oxygen free radicals and proteases [39,40]. In this study, MEOg (50 and 100 mg/kg) significantly ameliorated the increase in TNF-α level in carrageenan-induced inflammation in the rat pouch.…”

Section: Discussionmentioning

confidence: 99%

Ocimum gratissimum Linn. Leaf extract inhibits free radical generation and suppressed inflammation in carrageenan-induced inflammation models in rats

Ajayi

Ologe

Ben‐Azu

et al. 2017

Journal of Basic and Clinical Physiology and Pharmacology

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Background: Ocimum gratissimum leaf is used in managing rheumatism and other inflammatory conditions. In this study, we investigated the antioxidant and anti-inflammatory effects of phenolic extract obtained by sequential methanol extraction of O. gratissimum leaves (MEOg). Methods: The methanol extract (MEOg) was obtained after sequential maceration (n-hexane, chloroform and methanol) of dried O. gratissimum leaves. The fingerprint of the extract was obtained using a high-performance liquid chromatrographic method. In vitro effects were tested by 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide (NO) free radical scavenging, lipoxygenase, and xanthine oxidase inhibitory assays. MEOg was studied for anti-inflammatory activity in carrageenan-induced paw edema and air pouch inflammation in rats. Results: HPLC fingerprint of the extract shows the presence of caffeic acid, rutin, ferulic acid, apigenin, and quercetin.

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Protected effect of Esenbeckia leiocarpa upon the inflammatory response induced by carrageenan in a murine air pouch model

Cited by 23 publications

References 26 publications

Activation of human neutrophils by Esenbeckia leiocarpa: comparison between the crude hydroalcoholic extract (CHE) and an alkaloid (Alk) fraction

Activation of human neutrophils by Esenbeckia leiocarpa: comparison between the crude hydroalcoholic extract (CHE) and an alkaloid (Alk) fraction

Intravenous Fat Emulsion Formulations for the Adult and Pediatric Patient

Ocimum gratissimum Linn. Leaf extract inhibits free radical generation and suppressed inflammation in carrageenan-induced inflammation models in rats

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