Protease and lipase production by a strain ofSerratia marcescens (532 S)

Henriette, C.; Zinebi, Sanae; Aumaitre, M. F.; Petitdemange, E.; Petitdemange, H.

doi:10.1007/bf01569913

Cited by 37 publications

(26 citation statements)

References 19 publications

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“…Proteases production by S. marcescens IBB Po15 control cells and cells exposed to 5% organic solvents was further investigated. Bacterial cultures (20 μl) after organic solvents exposure were spotted on proteose peptone agar 26 ). Petri plates were incubated for 24 hours at 30°C.…”

Section: Resultsmentioning

confidence: 99%

“…Protease activity was lower (below 75%) after cyclohexane, n-hexane, toluene, styrene, and ethylbenzene exposure of S. marcescens IBB Po15 cells, compared with the control and n-decane exposed cells (100%). Henriette et al 26 reported that the production of protease by Serratia marescens 532 S was influenced by nutritional factors which acted as inducers or repressors.…”

Section: Resultsmentioning

confidence: 99%

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Highly Solvent Tolerance in Serratia marcescens IBBPo15

Stancu

2016

Braz. arch. biol. technol.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Highly Solvent Tolerance in Serratia marcescens IBBPo15

Stancu

2016

Braz. arch. biol. technol.

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“…Olive oil was also used successfully to increase lipase production by several investigators using different microorganisms (SILVA et al 2005, THOMAS et al 2003, CIHANGIR and SARIKAYA 2004, TAN et al 2004, SUGIHARA et al 1990. Tributyrin had been successfully used to increase lipase production by microorganisms, such as Issatchenkia orientalis, Pseudomonas sp., Lactobacillus casei, Serratia marcescens, Alternaria brassicicola and Rhizopus delemar (COSTAS et al 2004, KANWAR et al 2002, CHOI and LEE, 2001, HENRIETTE et al 1993, BERTO et al 1997, ESPINOSA et al 1990). In contrast, NAHAS et al (1988) reported lower lipase production by Rhizopus oligosporus in oilsupplemented medium.…”

Section: Effect Of Oilmentioning

confidence: 93%

Sequential parametric optimization of lipase production by a mutant strain Rhizopus sp. BTNT‐2

Bapiraju

Sujatha

Ellaiah

et al. 2005

J. Basic Microbiol.

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Lipase production by the mutant strain Rhizopus sp. BTNT-2 was optimized in submerged fermentation. Different chemical and physical parameters such as carbon sources, nitrogen sources, oils, inoculum level, pH, incubation time, incubation temperature and aeration have been extensively studied to increase lipase productivity. Potato starch (1.25% w/v) as a carbon source, corn steep liquor (1.5% w/v) as a nitrogen source and olive oil (0.5% v/v) as lipid source were found to be optimal for lipase production. The optimal levels of other parameters are 4 ml of inoculum (2.6x10(8) spores/ml), initial pH of 5.5, incubation time of 48 hours, incubation temperature of 28 degrees C and aeration rate of 120 rpm. With the optimized parameters, the highest production of lipase was 59.2 U/ml while an yield of only 28.7 U/ml was obtained before optimization resulting in 206% increase in the productivity.

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“…After incubation, the biomass concentration was analyzed regularly at every 6 h intervals up to 36 h using spectrophotometer at 600 nm [17]. The carrying out the assay in triplicate and average of the experiment carried out 3 times, and the average values were taken.…”

Section: Effect Of Temperature On Growthmentioning

confidence: 99%

Isolation, Purification, and Characterization of Lipase From Bacillus Sp. From Kitchen Grease

Jaiganesh

2018

Asian J Pharm Clin Res

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Objective: The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. from kitchen grease for a variety of applications including organic synthetic reactions and preparation of enantiomerically pure pharmaceuticals.Methods: Lipase producing isolates were screened from kitchen grease on a selective medium rhodamine B olive oil agar, and tributyrin agar was used to screen the lipase and esterase producing an organism, respectively. The isolate identified using 16S rDNA sequencing method and enzyme activity was quantitatively assayed. Lipase production was characterized in different conditions. Results:The isolate showed highest lipase activity was which later was identified as Bacillus sp. using 16S rDNA sequencing method. The lipase was purified using ammonium sulfate precipitation. The isolate showed excellent tolerance to methanol, ethanol, acetonitrile, and moderate tolerance to butanol. The increased biomass concentration, maximum production, and activity were achieved at 37°C in 24 h incubation, then gradual reduction in production was observed. The maximum activity of lipase enzyme was obtained at pH between 6 and 9. Conclusion:The isolate produce solvent tolerance lipase enzyme and it can be a promising candidate of solvent tolerance lipase enzyme for variety of industrial applications.

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Protease and lipase production by a strain ofSerratia marcescens (532 S)

Cited by 37 publications

References 19 publications

Highly Solvent Tolerance in Serratia marcescens IBBPo15

Highly Solvent Tolerance in Serratia marcescens IBBPo15

Sequential parametric optimization of lipase production by a mutant strain Rhizopus sp. BTNT‐2

Isolation, Purification, and Characterization of Lipase From Bacillus Sp. From Kitchen Grease

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