Sequential parametric optimization of lipase production by a mutant strain <i>Rhizopus</i> sp. BTNT‐2

Bapiraju, K. V. V. S. N.; Sujatha, P.; Ellaiah, P; Ramana, T.

doi:10.1002/jobm.200410548

Cited by 21 publications

(15 citation statements)

References 67 publications

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“…Similar effects were reported on lipase production in Rhizopus sp. BTNT-2 [3] and Yarrowia lipolytica [13]. In contrast, the addition of surfactant to the culture medium has been shown to increase the secretion of lipolytic enzymes in a number of microorganisms, attributable to alteration of cell permeability leading to increased protein secretion or to surface effects on cell-bound enzymes [39].…”

Section: Discussionmentioning

confidence: 93%

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Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Dheeman¹,

Frias²,

Henehan³

2009

J Ind Microbiol Biotechnol

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23Among several lipase producing actinomycete strains screened, Amycolatopsis 24 mediterranei DSM 43304 was found to produce a thermostable, extracellular lipase. 25Culture condition and nutrient source modification studies involving carbon sources, 26 nitrogen sources, incubation temperature and medium pH were carried out. Lipase activity 27 of 1.37 ± 0.103 IU/ml of culture medium was obtained in 96 h at 28 °C and pH 7.5 using 28 linseed oil and fructose as carbon sources and a combination of phytone peptone and yeast 29 extract (5:1) as nitrogen sources. In optimal culture conditions the lipase activity was 30 enhanced 12-fold with a 2-fold increase in lipase specific activity. The lipase showed 31 maximum activity at 60 °C and pH 8.

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Section: Discussionmentioning

confidence: 93%

“…Actinomycete strains were grown on GYM agar slants (g/l: glucose 4.0 g; yeast extract 4.0 g; malt extract 10 g; CaCO 3 2.0 g; agar 12.0 g; pH 7.2). The working stock cultures were maintained and stored on GYM slants at 4°C.…”

Section: Maintenance Of Microorganismsmentioning

confidence: 99%

Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Dheeman¹,

Frias²,

Henehan³

2009

J Ind Microbiol Biotechnol

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“…This study was also supported by the positive effect of olive oil, tributyrin, coconut oil and castor oil on lipase production by Rhizopus sp. BTNT-2 (Bapiraju et al 2005). In the present study, the effect of different concentrations of cod liver oil on lipase production showed a positive linear increase (11.2-15.53 U/ml) as the concentration of cod liver oil increased from 0.5 to 1.5%.…”

Section: Effect Of Various Triglycerides On Lipase Productionmentioning

confidence: 97%

Lipase production by Staphylococcus epidermidis CMST-Pi 1 isolated from the gut of shrimp Penaeus indicus

et al. 2010

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A bacterium producing extracellular lipase was isolated from the gut of the estuarine shrimp Penaeus indicus and identified as Staphylococcus epidermidis. Lipase production was investigated in shake-flask culture. Cod liver oil was found to be a suitable triglyceride to increase lipase production, at an optimum concentration of 1.5%. Investigating the effect of carbon source on lipase production revealed that fructose yielded higher lipase production than the other tested carbon sources; the optimal concentration of fructose was 2.5%. Screening of a suitable nitrogen source for lipase production revealed that meat extract yielded higher lipase production; the optimal concentration of meat extract for higher lipase production was 2.0%. The most suitable trace element for maximum lipase production was zinc sulphate, followed by magnesium sulphate. Partial characterization of the crude lipase revealed that pH 7 and a temperature of 50°C gave optimal lipase activity. Testing the effect of various organic solvents on lipase activity revealed that hexadecane (77%), tetradecane (74%) and dodecane (48%) increased lipase activity over the control.

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“…In contrast, the addition of vegetable oils (olive, corn, and soybean oil) did not significantly affect lipase production. Bapiraju et al (2005) optimized the lipase production by the mutant strain Rhizopus sp. BTNT-2 in SmF.…”

Section: Agroindustrial Residuesmentioning

confidence: 99%

“…Bapiraju et al (2005) optimized the lipase production by the mutant strain of Rhizopus sp. and the optimum activity was 29 U mL −1 .…”

Section: Filamentous Fungimentioning

confidence: 99%

A Review on Microbial Lipases Production

Treichel

Oliveira

Mazutti

et al. 2009

Food Bioprocess Technol

423

222

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This review paper provides an overview regarding the main aspects of microbial lipases production. The most important microbial lipase-producing strains for submerged and solid-state fermentations are reviewed as well as the main substrates, including the use of agroindustrial residues. Current process techniques (batch, repeated-batch, fed-batch, and continuous mode) are discussed and the main bioreactors configurations are also presented. Furthermore, the present review paper shows a general overview about the development of mathematical models applied to lipase production. Finally, some future perspectives on lipase production are discussed with special emphasis on lipase engineering and the use of mathematical models as a useful tool for process improvement and control.

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Sequential parametric optimization of lipase production by a mutant strain Rhizopus sp. BTNT‐2

Cited by 21 publications

References 67 publications

Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Lipase production by Staphylococcus epidermidis CMST-Pi 1 isolated from the gut of shrimp Penaeus indicus

A Review on Microbial Lipases Production

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