Protaetia brevitarsis seulensis Derived Protein Isolate with Enhanced Osteomodulatory and Antioxidative Property

Abstract

The osteogenic differentiation of stem cells is profoundly affected by their microenvironmental conditions. The differentiation behavior of stem cells can be tuned by changing the niche environments. The proteins or peptides that are derived by living organisms facilitate the osteogenic differentiation of stem cells. Here, we have evaluated the osteoinductive and antioxidative potential of the Protaetia brevitarsis seulensis insect-derived protein for human bone marrow-derived mesenchymal stem cells (hBMSCs). The amino acid contents in the isolated protein were determined by an amino acid analyzer. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to analyze the extract’s functional groups and surface morphology. The extracted protein exhibited 51.08% β-sheet conformation. No adverse effects were observed in extract-treated cells, indicating their biocompatibility. The protein isolate showed an excellent antioxidative property. Besides this, an enhancement in the hBMSCs’ mineralization has been observed in the presence of treated protein isolates. Notably, osteogenic marker genes and proteins were effectively expressed in the treated cells. These results indicated that the P. brevitarsis-derived protein isolate can be used as a potential antioxidative biomaterial for bone tissue engineering applications.

Bioengineered Lab-Grown Meat-like Constructs through 3D Bioprinting of Antioxidative Protein Hydrolysates

“…The media with only 5% FBS was taken as the control. The staining procedure was described in our previous studies. , Briefly, the cells were fixed with 3.7% paraformaldehyde (PFA) for 15 min at room temperature, followed by permeabilization with Triton-X 100 (0.1%) for 10 min. After that, the cells were blocked by 1% BSA for 1 h and incubated with specific primary and secondary antibodies against desmin and myogenin.…”

“…PHs contain several bioactive peptides that act as stress-reducing agents. PHs can be obtained from various protein sources such as mushrooms, fish fins and scales, , porcine skin, soybeans, milk, , pigeon peas, potatoes, and edible insects . In addition, PHs (peptide fragments or cryptides) are widely recognized as antioxidative molecules present inside cells .…”

Osteo‐modulatory dietary proteins and peptides: A concise review

“…The popular ones include edible insects and marine organisms, among others (Van Huis, 2013). Recently, peptides from Protaetia brevitarsis insect‐derived protein have been shown to enhance osteogenic differentiation of bone stem cells in humans (Ganguly et al, 2020). In addition, algae and microalgae contain substantial amounts of proteins and are considered a sustainable protein sources for both human and animal nutrition (Nwachukwu & Aluko, 2021; Sheih et al, 2010).…”

The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells

“…In addition, the clinical studies have reported AG administration significantly improved the CD4+ lymphocyte count in HIV-positive patients [28] and inhibited the gp120-mediated cell fusion of HL2/3 cells with TZM-bl cells [29]. Moreover, publications related with the effects of natural molecules on osteogenesis of stem cells has been currently increased [30][31][32]. Stimulating bone and cartilage formation with biologically active pharmaceuticals derived from natural sources is a direction worth investigating for the treatment of homeostasis of bone diseases and/or bone remodeling in regenerative medicine field.…”