Prostatic tissue distribution of 17β-hydroxy-5α-androstan-3-one and of androgen receptors in benign hyperplasia

Sirett, D.A.N.; Cowan, Sheila K.; Janeczko, Anna; Grant, J. K.; Glen, E.S.

doi:10.1016/0022-4731(80)90222-8

Cited by 45 publications

(19 citation statements)

References 22 publications

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“…However, using a combined biochemical and morphometric approach, Schweikert et al (184) and Bartsch et al (185) report equal levels of 5a-reductase in prostatic epithelium and stroma. Androgen receptors have been shown by both biochemical and autoradiographic procedures to be in both epithelial and stromal cells (86,180, [186][187][188][189][190].…”

Section: Regulation Of Prostatic Growthmentioning

confidence: 98%

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The Endocrinology and Developmental Biology of the Prostate*

et al. 1987

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Section: Regulation Of Prostatic Growthmentioning

confidence: 98%

“…Although it is unclear whether androgen receptors are elevated in one or the other cell fraction, both cell types are now accepted as bona fide androgen targets (86,180, [186][187][188][189][190][191][192]. Estrogen and progestin receptors have also been detected in both epithelium and stroma (173,189).…”

Section: Regulation Of Prostatic Growthmentioning

confidence: 99%

The Endocrinology and Developmental Biology of the Prostate*

et al. 1987

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“…Androgen metabolism (Harper et al 1974;Cowan et al 1977Cowan et al , 1979Bruchovsky et al 1980;Romijn et al 1980;Krieg et al 1980Krieg et al , 1981, androgen and oestrogen binding (Bashirelahi et al 1980;Sirett et al 1980;Krieg et al 1981) and endogenous tissue DHT levels (Bruchovsky et al 1980;Sirett et al 1980;Bolton et al 1981) have been investigated in both compartments of BPH. With respect to androgen metabolism, most investigators came to stroma than in the epithelium of BPH, while in the normal prostate (Bruchovsky et al 1980;Krieg et al 1981) this difference is less pronounced.…”

mentioning

confidence: 97%

“…With respect to androgen metabolism, most investigators came to stroma than in the epithelium of BPH, while in the normal prostate (Bruchovsky et al 1980;Krieg et al 1981) this difference is less pronounced. Along this line, it has been speculated that the pathologi¬ cal alterations in BPH might be induced by in¬ creased DHT formation in the stroma, which in turn could stimulate the growth of the adjacent epithelium (Sirett et al 1980).…”

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confidence: 99%

Endogenous androgen levels in epithelium and stroma of human benign prostatic hyperplasia and normal prostate

Bartsch¹,

Krieg²,

Becker³

et al. 1982

Acta Endocrinologica

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5\g=a\-Dihydrotestosterone (DHT) and 5\g=a\-androstane-3\g=a\, 17\g=b\-diol(3\g=a\-diol) were extracted from epithelium and stroma of human benign prostatic hyperplasia (BPH) and of normal prostate and quantified by RIA. The main results were: (1) concerning the BPH, DHT is mainly located in the nuclear fraction of epithelium and stroma, whereas 3\g=a\-diolwas completely of extranuclear origin, (2) in the nuclei derived from BPH stroma the DHT content (7.1 \ m=+-\0.88 pmol/mg DNA, mean \m=+-\sem [n = 14]) was significantly higher (P < 0.01) than in the nuclei derived from BPH epithelium (3.8 \ m=+-\ 0.38 pmol/ mg DNA [n = 14]). The DHT content in the nuclear fraction derived from unseparated, i.e. whole tissue, was 5.6 \m=+-\0.60 pmol/mg DNA [n = 14], (3) the biological significance of the overwhelming DHT accumulation in the stromal nuclei for the BPH tissue is reflected by a significant correlation between the DHT values in the nuclei from stroma and whole tissue (rs = 0.710, P < 0. 01) and (4) in four normal prostates the DHT content in the nuclear fraction of epithelium (1.3 \m=+-\0.37

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“…[23] separated epithelium and stroma of human BPH tissue and found that cytosolic androgen-binding sites were evenly distributed beween epithelial and stromal cells. Also, Sirett et al [24] measured nuclear androgen recep tors in both epithelium and stroma and found no sub stantial enrichment of receptors in epithelial cells as com pared to the stromal compartment. From these results it seems to be established that androgen receptors are present in stromal cells of human BPH in comparable amounts as in epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Determination of Androgen Receptors in Human Benign Prostatic Hypertrophy with Two Synthetic Radiolabeled Ligands

Schwemmer¹,

Böttger²,

Schiessl

et al. 1986

Urol Int

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Nuclear and cytosolic androgen receptor concentrations in tissues of human benign prostatic hypertrophy (BPH) were determined by use of methyltrienolone (R-1881) and 7Α,17Α-dimethyl-19-nortestosterone (DMNT) as radiolabeled ligands. Cytosolic R-1881-binding sites were 46.1 ± 43 fmol/mg protein and nuclear R-1881-binding sites were 51.8 ± 42 fmol/mg protein. DMNT-binding sites in cytosol were 44.3 ± 38 fmol/mg protein and in nuclear extract 73.4 ± 64 fmol/mg protein. No significant correlation was found between the number of R-1881 – and DMNT-binding sites in either cytosol or nuclear extracts. Cytosolic or nuclear androgen receptor content was not significantly correlated with the percentage of epithelial or stromal cells as determined from the corresponding histological sections. In BPH tissue with marked cystic degeneration, very low androgen receptor levels were found.

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Prostatic tissue distribution of 17β-hydroxy-5α-androstan-3-one and of androgen receptors in benign hyperplasia

Cited by 45 publications

References 22 publications

The Endocrinology and Developmental Biology of the Prostate*

The Endocrinology and Developmental Biology of the Prostate*

Endogenous androgen levels in epithelium and stroma of human benign prostatic hyperplasia and normal prostate

Determination of Androgen Receptors in Human Benign Prostatic Hypertrophy with Two Synthetic Radiolabeled Ligands

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