Prostate Stem Cells and Prostate Cancer

Lawson, Devon A.; Li, Xin; Lukacs, Rita U.; Xu, Qiong; Cheng, Donghui; Witte, Owen N.

doi:10.1101/sqb.2005.70.003

Cited by 37 publications

(22 citation statements)

References 70 publications

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“…Sca-1 ϩ cells possess several properties of stem cells, including replication quiescence, multilineage differentiation capacity, and localization in the region of prostatic ducts proximal to the urethra that has been identified as the prostate stem cell niche. Sca-1 also is expressed in fetal prostate epithelium, demonstrating it is expressed by prostate stem cells from very early stages of prostate development (12). Here, we demonstrate that the CD45 Ϫ CD31 Ϫ Ter119 Ϫ Sca-1 ϩ CD49f ϩ prostate cell subpopulation is enriched for cells capable of both colony and sphere formation in vitro.…”

mentioning

confidence: 69%

“…Experiments in which mixtures of differentially marked cells were implanted in the in vivo prostate regeneration system have demonstrated that regenerated tubules are of clonal origin because chimeric tubules are observed rarely (11,12,32). Likewise, no chimeric tubules containing both dsRED ϩ and GFP ϩ cells were observed in these experiments (SI Fig.…”

Section: Lsc Cells Can Differentiate To Produce Prostatic Tubule Strumentioning

confidence: 88%

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Isolation and functional characterization of murine prostate stem cells

Lawson

Lukacs

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

354

425

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The ability to isolate prostate stem cells is essential to explore their role in prostate development and disease. In vitro prostate colonyand sphere-forming assays were used to quantitatively measure murine prostate stem/progenitor cell enrichment and self-renewal. Cell surface markers were screened for their ability to positively or negatively enrich for cells with enhanced growth potential in these assays. Immunohistochemical and FACS analyses demonstrate that specific cell surface markers can be used to discriminate prostate stromal (CD34 ؉ ), luminal epithelial (CD24 ؉ CD49f ؊ ), basal epithelial (CD24 ؉ CD49f ؉ ), hematopoietic (CD45 ؉ , Ter119 ؉ ), and endothelial (CD31 ؉ ) lineages. Sorting for cells with a CD45 ؊ CD31 ؊ Ter119 ؊ Sca-1 ؉ CD49f ؉ antigenic profile results in a 60-fold enrichment for colony-and sphere-forming cells. These cells can self-renew and expand to form spheres for many generations and can differentiate to produce prostatic tubule structures containing both basal and luminal cells in vivo. These cells also localize to the basal cell layer within the region of the gland that is proximal to the urethra, which has been identified as the prostate stem cell niche. Prostate stem cells can be isolated to a purity of up to 1 in 35 by using this antigenic profile. The remarkable similarity in cell surface profile between prostate and mammary gland stem cells suggests these markers may be conserved among epithelial stem cell populations.CD49f ͉ integrin ␣6 ͉ Sca-1 ͉ CD24 heat-stable antigen ͉ stem cell niche S tem cells are of interest clinically because of their potential to repair damaged tissues, treat degenerative diseases, and because of their purported role in tumor initiation. The ability to identify and isolate stem cells is necessary to study their specialized biology. Enrichment for many types of tissue stem cells has been achieved by using cell surface markers. Murine hematopoietic stem cells can be enriched by sorting Lin Ϫ Thy-1 lo Sca-1 ϩ ckit ϩ cells from the bone marrow (1). Recent studies suggest that even better purity can be achieved by further sorting based on expression of the SLAM family receptors CD150 and CD48 (2). Bronchioavelolar stem cells can be isolated from their niche at the bronchioalveolar duct junction (BADJ) by sorting cells with a CD45 Ϫ CD31 Ϫ Sca-1 ϩ CD34 ϩ profile (3). Data from two recent reports show that mouse mammary stem cells possess a Lin Ϫ Sca-1 ϩ CD140a Ϫ CD24 ϩ CD49f ϩ CD29 ϩ cell surface profile and can be isolated to a purity of up to 1 in 20 by using subsets of these markers (4, 5).The presence of stem cells in the prostate first was proposed to explain the seemingly inexhaustible capacity of the organ to regenerate during androgen cycling experiments (6). The identification of side-population cells and replication quiescent BrdU label-retaining cells further suggests that stem cells exist in the gland (7,8). Several studies have enriched for primitive prostate cells by using cell surface markers. Richardson et al. (9) demonstrated that th...

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mentioning

confidence: 69%

Section: Lsc Cells Can Differentiate To Produce Prostatic Tubule Strumentioning

confidence: 88%

Isolation and functional characterization of murine prostate stem cells

Lawson

Lukacs

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

354

425

View full text Add to dashboard Cite

show abstract

“…Prostate tissue can also be regenerated when dissociated cells are implanted s.c. in Matrigel or in collagen onto the flanks of immunodeficient mice (59,60). Experiments utilizing mixed populations of differentially marked epithelial cells have demonstrated that the tubules regenerated using these protocols are clonal, as chimeric tubules expressing both markers are rarely observed (60)(61)(62). Immunohistochemical analyses have shown that each duct contains both basal and luminal cell lineages (58,(60)(61)(62).…”

Section: Development Of Prostate Epithelial Stem Cell Assaysmentioning

confidence: 99%

Stem cells in prostate cancer initiation and progression

Lawson

Witte²

2007

J. Clin. Invest.

154

130

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“…The heterogeneity of cancer stem cells accounts for the different molecular characteristics of each individual tumour, therefore for successful treatment, the cancer stem cells need to be targeted. CD133 has been identified as a beneficial cancer stem cell marker, however the small population of cancer stem cells limits the amount of marker profiling that can be carried out (Lawson et al, 2005).…”

Section: Pathogenesis Of Prostate Cancermentioning

confidence: 99%