Prostate specific antigen molecular forms in breast cyst fluid and serum of women with fibrocystic breast disease

Borchert, Gudrun H.; Yu, He; Tomlinson, George; Giai, M; Roagna, Riccardo; Ponzone, Riccardo; Sgrò, Luca; Diamandis, Eleftherios P.

doi:10.1002/(sici)1098-2825(1999)13:2<75::aid-jcla6>3.0.co;2-f

Cited by 16 publications

(11 citation statements)

References 20 publications

(33 reference statements)

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“…Also, high PEPC levels in breast cyst fluid were found in women who were aspirated on the first half of their menstrual cycle. Recently, it was demonstrated that the production and intracystic accumulation of PSA, and in particular the free form of PSA, was associated with the apocrine/Type I cyst (13,24). In this report, we also associated increased levels of PEPC in cyst fluid with the apocrine/Type I cyst.…”

Section: Discussionsupporting

confidence: 63%

“…Cyst fluids were centrifuged and the supernatants were collected and stored at Ϫ20°C until analysis. Our clinical specimens were leftovers from a previous study examining if free (F-PSA) or total PSA levels in cyst fluids and matched sera associate with the type of breast cyst (13).…”

Section: Clinical Specimensmentioning

confidence: 99%

“…Although the involvement of PSA in the physiologic and pathogenic mechanisms of tissue development and tumorigenesis has not been elucidated yet (7), we have provided evidence that qualifies PSA as a prognostic indicator in breast cancer and supports its use for patient prognosis and selection of therapy (8 -11). Recently, we have quantified the PSA immunoreactivity in breast cyst fluid and suggested that this marker has some value for cyst subclassification (12,13).…”

Section: Introductionmentioning

confidence: 99%

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Quantification of pepsinogen C and prostaglandin D synthase in breast cyst fluid and their potential utility for cyst type classification

Borchert

Melegos

et al. 1999

Clinical Biochemistry

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Section: Discussionsupporting

confidence: 63%

Section: Clinical Specimensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantification of pepsinogen C and prostaglandin D synthase in breast cyst fluid and their potential utility for cyst type classification

Borchert

Melegos

et al. 1999

Clinical Biochemistry

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“…Surprisingly, we found that PSA RNA-transfected DC generated from healthy males, females, and cancer patients stimulated CTL responses of similar magnitude. One possible explanation is the fact that PSA expression, albeit at very low levels, can be found in some tissues in normal females (20,21). Indeed, anti-PSA Abs have been detected in some healthy females, suggesting that PSA can be the target of a humoral autoimmune response (22).…”

Section: Discussionmentioning

confidence: 99%

Human Dendritic Cells Transfected with RNA Encoding Prostate-Specific Antigen Stimulate Prostate-Specific CTL Responses In Vitro

Heiser¹,

Dahm²,

Yancey³

et al. 2000

The Journal of Immunology

174

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Although immunological tolerance to self Ags represents an important mechanism to prevent normal tissue injury, there is growing evidence that tolerance to tumor Ags, which often represent normal peripherally expressed proteins, is not absolute and can be effectively reverted. Prostate-specific Ag (PSA) is a self Ag expressed by both normal and malignant prostatic epithelium, and therefore offers a unique opportunity to examine the ability of self Ags to serve as specific CTL targets. In this study, we investigated the efficacy of autologous dendritic cells (DC) transfected with mRNA encoding PSA to stimulate CTL against PSA Ags in vitro. Ag in form of RNA carries the advantage to encode multiple epitopes for many HLA alleles, thus permitting induction of CTL responses among many cancer patients independent of their HLA repertoire. In this study, we show that PSA mRNA-transfected DC were capable of stimulating primary CTL responses against PSA Ags in vitro. The PSA-specific CTL did not cross-react with kallikrein Ags, a protein, which shares significant homology with PSA, suggesting that harmful autoimmune toxicity may not represent a significant problem with this approach. PSA RNA-transfected DC generated from male or female healthy volunteers or from cancer patients were equally effective in stimulating PSA-specific CTL in vitro, implying that neither natural tolerance to PSA Ags nor tumor-mediated T cell anergy may represent major barriers for CTL generation against the self Ag PSA. This study provides a preclinical rationale for using PSA RNA-transfected DC in active or adoptive immunization protocols.

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“…This finding is in accordance with in-situ hybridization studies in which it was found that the level of PSA expression in the prostate tumor mass is inversely proportional to the tumor grade (Qiu et al 1990). ␣-1-antichymotrypsin, a protein known to bind PSA (Borchert et al 1999), shows a similar expression pattern.…”

Section: Example Analysismentioning

confidence: 95%

The Comparison of Gene Expression from Multiple cDNA Libraries

Stekel

Git²,

Falciani³

2000

Genome Research

228

312

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Prostate specific antigen molecular forms in breast cyst fluid and serum of women with fibrocystic breast disease

Cited by 16 publications

References 20 publications

Quantification of pepsinogen C and prostaglandin D synthase in breast cyst fluid and their potential utility for cyst type classification

Quantification of pepsinogen C and prostaglandin D synthase in breast cyst fluid and their potential utility for cyst type classification

Human Dendritic Cells Transfected with RNA Encoding Prostate-Specific Antigen Stimulate Prostate-Specific CTL Responses In Vitro

The Comparison of Gene Expression from Multiple cDNA Libraries

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