Prostaglandin E2 opunteracts the effects of PGF2α in indomethacin treated cycling gilts

Akinlosotu, B.A.; Diehl, John R.; Giménez, T.

doi:10.1016/0090-6980(88)90276-6

Cited by 57 publications

(30 citation statements)

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“…The role of PGE2 in corpus luteum function during early preg¬ nancy of pigs is unknown. However, PGE2 can counteract the luteolytic effect of PGF2a, since chronic intrauterine infusions of PGE2 from day 7 in cyclic gilts (Akinlosotu et al, 1986(Akinlosotu et al, , 1988, and day 9 in cows (Giménez & Henricks, 1981) and ewes (Pratt et ai, 1979), inhibits luteolysis, main¬ tains luteal progesterone production and prolongs the oestrous cycle. Shelton et al (1990) Our studies also demonstrated that LH and prostaglandins F2a and E2 interact to inhibit progesterone production by pig luteal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Other prostaglandins, such as E2, I2 and D2 are also produced by luteal tissue and prolong the lifespan of the corpus luteum in ewes (Huecksteadt & Weems, 1978), cows (Milvae & Hansel, 1983;Alila et al, 1988) and humans (Bennegard et al, 1990). Prostaglandin E2 (PGE2) also counteracts the effects of PGF2a in indomethacin-treated cyclic gilts (Akinlosotu et al, 1986(Akinlosotu et al, , 1988, but the mechanism of action of prostaglandins on luteal function in pigs is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Effect of prostaglandins on luteal function during early pregnancy in pigs

Więsak

Hunter²,

Foxcroft³

1992

Reproduction

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Summary. Luteal cells were obtained by digestion of luteal tissue of cyclic (day 12) and early pregnant (days 12, 20 and 30) pigs. Suspensions of the dispersed luteal cells (5 x 104 cells ml \m=-\1) were incubated for 2 h in minimum essential medium (MEM) alone (control) and MEM with different concentrations of prostaglandin FF2\ g=a\(PGF2\g=a\) and PGE2 (0\m=.\01,0\m=.\1,1, 10, 100 and 1000 ng ml \m=-\1) and luteinizing hormone (LH) 100 and 1000 ng ml\m=-\1, or with combinations of LH+ PGF2\g=a\and LH + PGE2. Net progesterone production was measured in the incubation media by direct radioimmunoassay.The overall response pattern of the luteal cells to exogenous hormones on day 12 of the oestrous cycle and pregnancy differed (P < 0\m=.\5) from treatment on day 20 and 30 of pregnancy. In general progesterone production was higher (P < 0\m=.\5) and the response to PGF2\g=a\ and PGE2 treatment was most obvious on day 12 of the oestrous cycle and pregnancy. Overall, PGF2\g=a\ stimulated progesterone production in a dosedependent manner (P < 0\m=.\05). The response to PGE2 was of a quadratic nature (P < 0\m=.\05) in which the lowest and the highest doses of PGE2 were associated with a greater production of progesterone than were the intermediate doses. Treatment of luteal cells with PGF2\g=a\ + LH or PGE2 + LH caused overall inhibition (P < 0\m=.\05) of progesterone production compared with treatment with each hormone alone. This interaction was not affected by the dose of LH used.These findings indicate that PGF2\g=a\ and PGE2 are involved in the autocrine control of corpus luteum function.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of prostaglandins on luteal function during early pregnancy in pigs

Więsak

Hunter²,

Foxcroft³

1992

Reproduction

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“…Einspannier et al [10] [12]. The presence of the blastocyst probably also acts to alter the final product such that the primary prostaglandin released is PGE 2 rather than PGF Za [1,28]. As a matter of fact, PGE 2 neutralised the luteolytic effect of simultaneously infused PGFZa in indomethacin-treated gilts [1] with fewer PGF Za secretion spikes, and with a lower amplitude than has been reported for pregnant, as compared to cyclic gilts [28].…”

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confidence: 96%

Luteinising hormone receptor kinetic and LH-induced prostaglandin production throughout the oestrous cycle in porcine endometrium

Stępień¹,

Shemesh²,

Ziȩcik³

1999

Reprod. Nutr. Dev.

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“…Oxytocin, acting through oxytocin receptors (OTR) in the endometrium and myometrium, is involved in the control of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) secretion in pigs [3][4][5][6][7][8]. Prostaglandin F2α is responsible for luteal regression in non-pregnant pigs [1,9], while prostaglandin E2 acts as a luteotrophic and antiluteolytic factor that promotes maintenance of the corpus luteum during early pregnancy in pigs [10,11].Alteration of PG secretion by OT is implicated in maternal recognition of pregnancy in pigs [12][13][14]. Moreover, OT stimulates myometrial secretion of PGF2α and PGE2 as well as the contractile activity of the porcine myometrium during early pregnancy [8,15,16].…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

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Abstract. In the pig, the periimplantation period is critical for successful establishment of pregnancy. We studied the influence of embryos on oxytocin (OT) and progesterone (P4) regulated endometrial and myometrial secretion of 1) luteotrophic prostaglandin E2 (PGE2) and 2) luteolytic prostaglandin F2α and its metabolite (PGFM) on days 12-14 of pregnancy in pigs. We used unilaterally pregnant pigs created by a surgical procedure in which one uterine horn remained intact and the second horn was cut transversely so that part of the horn was detached from the uterine body. The animals were divided into two groups, inseminated gilts (days 12-14 of pregnancy, n=6) and uninseminated cyclic gilts, which were used as controls (days 12-14 of estrous cycle, n=5). Embryos developed only in the patent part of the uterus and not in the occluded horn. The abundance of OTR mRNA was increased in the endometrium and decreased in the myometrium of the gravid uterine horn in the pregnant pigs compared with the non-gravid uterine horn or either uterine horn in the cyclic pigs, indicative of a local effect of the conceptus. The presence of embryos in the uterine horn during the periimplantation period determines endometrial metabolism of PGF2α and the local response of the endometrium to OT and P4. OT stimulates PGF2α secretion and PGFM accumulation in endometrial cultures only from the non-gravid uterine horn and controls PGE2 secretion from the endometrium and myometrium in the pregnant gilts. The results indicate a more systemic affect of pregnancy on the uterine response to OT and a possibly the local effect of the conceptus in promoting progesterone's inhibition of OT-stimulated PGE2 secretion and uterine metabolism of PGF2α. Key words: Embryos, Oxytocin, Pigs, Progesterone, Prostaglandin E2, Prostaglandin F2α and its metabolite, Uterus (J. Reprod. Dev. 55: [262][263][264][265][266][267][268][269][270][271][272] 2009) rostaglandins (PG) produced in the uterus and corpus luteum are necessary for successful reproduction [1,2]. Oxytocin, acting through oxytocin receptors (OTR) in the endometrium and myometrium, is involved in the control of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) secretion in pigs [3][4][5][6][7][8]. Prostaglandin F2α is responsible for luteal regression in non-pregnant pigs [1,9], while prostaglandin E2 acts as a luteotrophic and antiluteolytic factor that promotes maintenance of the corpus luteum during early pregnancy in pigs [10,11].Alteration of PG secretion by OT is implicated in maternal recognition of pregnancy in pigs [12][13][14]. Moreover, OT stimulates myometrial secretion of PGF2α and PGE2 as well as the contractile activity of the porcine myometrium during early pregnancy [8,15,16]. The uterine response to OT depends on expression of OTR mRNA and protein [7,[17][18][19] and on generation of the second messengers inositol triposhate and diacyloglycerol [7,18,20,21]. OT also stimulates PGF2α secretion from the bovine endometrium through a previously discussed signaling pathway [22][2...

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Prostaglandin E2 opunteracts the effects of PGF2α in indomethacin treated cycling gilts

Cited by 57 publications

References 25 publications

Effect of prostaglandins on luteal function during early pregnancy in pigs

Effect of prostaglandins on luteal function during early pregnancy in pigs

Luteinising hormone receptor kinetic and LH-induced prostaglandin production throughout the oestrous cycle in porcine endometrium

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

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