1983
DOI: 10.1007/bf02405038
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Prostaglandin E2 binding and cyclic AMP production in isolated bone cells

Abstract: The binding of prostaglandin E2 (PGE2) to bone cells was studied to provide direct evidence for the existence of specific receptors in bone. Bone cells were isolated by collagenase digestion of fetal and newborn rat calvaria. Isolated cells were incubated with 3H-PGE2 and collected on Millipore filters. Specific binding was determined by subtracting the binding that occurred with 10(-6) M non-radioactive PGE2 and 3H-PGE2 from that with 3H-PGE2 alone. With heterogeneous cell preparations and at PGE2 concentrati… Show more

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Cited by 30 publications
(9 citation statements)
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“…12, 2001 / 52-61 Prostaglandins are important mediators of osteoblastic differentiation (Dziak et al 1983;Nemoto et al 1997;Sabbieti et al 1999). Local regulation of bone formation occurs in a physiological background regulated by the endocrine system, including hormones such as 1,25-(OH) 2 D 3.…”
mentioning
confidence: 99%
“…12, 2001 / 52-61 Prostaglandins are important mediators of osteoblastic differentiation (Dziak et al 1983;Nemoto et al 1997;Sabbieti et al 1999). Local regulation of bone formation occurs in a physiological background regulated by the endocrine system, including hormones such as 1,25-(OH) 2 D 3.…”
mentioning
confidence: 99%
“…Indeed, the cell cultures used in the present study do not contain a homogeneous population, but osteoblastlike cells are in the majority, as indicated by a variety of biochemical criteria. Osteoblasts possess receptors for prostaglandins of the E series, and respond to prostaglandins with increases in cyclic AMP formation [4][5][6]. It is reasonable to suggest, therefore, that the cyclic AMP response in our experimental system stems mainly from osteoblasts (or osteoblastlike cells).…”
Section: Discussionmentioning
confidence: 95%
“…Rises in cellular cyclic AMP and Ca 2 § levels trigger a cascade of metabolic events that culminates in purposive cell behavior. There is reason to believe that prostaglandins of the "E" series (PGE) act in similar fashion to influence osteoblast function [4,5]; PGE binds specifically to osteoblastlike cells and stimulates cyclic AMP formation [6].…”
Section: Abstract: Bone Cells --Parathyroid Hormone Pge 2 --Adriamycmentioning
confidence: 99%
“…Even though high levels of prostaglandins are associated with inflammation, prostaglandins are important mediators of osteoblastic differentiation. PGE2 is needed for the increase of TGF-β1 and both molecules are needed in later stages for DPSCs terminal differentiation by increasing alkaline phosphatase specific activity [41][42][43] . In the present model an increase of PGE2 secretion level released by cells grown on test compared to those exposed to control surfaces was recorded, thus confirming the hypothesis of a more prominent osteoblastic differentiation on test surfaces.…”
Section: Discussionmentioning
confidence: 99%