Prostaglandin E₂Up-regulation and Wound Healing Effect of the Ethanol Extract of Eriobotryae Folium in Human Keratinocyte

Abstract

: Prostaglandin (PG) E 2 is an important mediator of skin wound healing without excessive scarring and gastric ulcer healing. However, PGE 2 has a short lifetime in vivo because it is metabolized rapidly by 15-hydroxyprostaglandin dehydrogenase (15-PGDH). Ethanol extract of Eriobotryae folium (EFEE) elevated intracellular and extracellular PGE 2 levels in HaCaT cells and inhibited 15-PGDH (ED 50 : 168.4 ㎍/mL) with relatively low cytotoxicity (IC 50 : 250.0 ㎍/mL). Real-time PCR analysis showed that mRNA expression of cyclooxygenase (COX)-1 and COX-2 enzymes were increased and prostaglandin transporter (PGT) was decreased in HaCaT cells by EFEE. Moreover, wound healing effect of EFEE (168.4 ㎍/mL) was comparable to that of TGF-β1 (300 pg/mL) as a positive control. These results demonstrate that EFEE may be valuable therapeutic materials for the treatment of PGE 2 level dependent diseases.

A Review of Wound Healing Mechanisms of Natural Products in Keratinocyte Cells

“…The leaves of Eriobotrya japonica contain amygdalin (laetrile and vitamin B1) which has antioxidant activity with an IC 50 value of 56.59 μg/ml (Figure 14). 43 The ethanolic extract of Eriobotryae folium (EF) increases intracellular and extracellular PGE2 levels in HaCaT cells and inhibits 15-PGDH (ED50: 168,4 μg/ml) with relatively low cytotoxicity (IC 50 : 250.0 μg/ml). In the other study, EF extract suppressed LPS-induced nitric oxide and PGE2 production by inhibiting inducible nitric oxide synthase and COX-2 expression in lipopolysaccharides that stimulated RAW264 cells decreased MRP4 and PGT expression.…”