Prostaglandin E
            <sub>1</sub>
            in Preparation and Storage of Platelet Concentrates

Becker, G. A.; Chalos, M. K.; Tuccelli, M.; Aster, Richard H.

doi:10.1126/science.175.4021.538

Cited by 28 publications

(3 citation statements)

References 4 publications

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“…Prostaglandin E 1 (PGE 1 ) is a reversible inhibitor of platelet activation because of its ability to increase cyclic AMP levels within the platelets . Although early studies showed that the addition of PGE 1 to human PRP facilitated the separation, concentration, and resuspension of platelets, there have been no studies of the effects of PGE 1 on the preparation of PC in dogs. The aim of this study was to investigate the efficacy of adding PGE 1 during PC preparation.…”

mentioning

confidence: 99%

Effects of Prostaglandin E₁on the Preparation of Platelet Concentrates in Dogs

Segawa

Kondo

Kimura

et al. 2012

Veterinary Internal Medicne

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Background: Platelet concentrates (PC) are prepared by centrifugation of platelet-rich plasma (PRP) that is prepared by centrifugation of whole blood. The resuspension of the platelet pellet during PC preparation from dogs is difficult because of platelet activation induced by centrifugation.Objectives: To investigate the efficacy of adding prostaglandin E 1 (PGE 1 ) to prevent platelet activation during PC preparation from dogs.Animals: Fifteen healthy Beagle dogs. Methods: Prospective, experimental trial: PGE 1 was added to PRP before the high-speed centrifugation during PC preparation. To estimate the effect of this addition, we assessed the platelet aggregability before transfusion, the survival of the platelets after transfusion, and the platelet reactivity after transfusion, which is estimated by the P-selectin expression of the platelets when stimulated by thrombin.Results: The difficulty associated with platelet resuspension was resolved by PGE 1. PGE 1 strongly inhibited platelet aggregation induced by collagen and ADP; however, it recovered after the platelets were resuspended in plasma without PGE 1 (mean aggregation ratio; collagen: 10.00-80.80%, ADP: 8.20-53.60%). Survival of the platelets after transfusion was not affected by PGE 1 (mean 8.04 and 7.56 days, without and with PGE 1 ), and thrombin-induced P-selectin expression after transfusion in PGE 1 -treated PC was also well maintained (mean positive ratio 53.7 and 47.9%, before and 24 hours after transfusion).Conclusions and Clinical Importance: The addition of PGE 1 in PRP before the centrifugation of PRP can improve the preparation efficiency of PC from dogs, while maintaining the therapeutic efficacy of the platelets.

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Effects of Prostaglandin E₁on the Preparation of Platelet Concentrates in Dogs

Segawa

Kondo

Kimura

et al. 2012

Veterinary Internal Medicne

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“…Because platelet activation is normally inhibited by factors that raise the level of cyclic adenosine 3′, 5′ monophosphate (cAMP), 9–11 attempts have been made, by the addition of cAMP‐activating agents, to improve and extend the storage of PCs for transfusion. These include adenylate cyclase stimulators, such as prostaglandin E 1 or forskolin, and camp phosphodiesterase inhibitors, such as theophylline or isobutyl‐methyl‐xanthine 12–17 . In addition, a solution made up of second‐messenger effectors, named ThromboSol, has recently been reported 18 to limit the known defects induced by refrigerated storage of PCs 19–26 and to lower cytokine accumulation and bacterial growth from that seen with PCs stored at 22°C 27 …”

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confidence: 99%

Quality assessment of platelet concentrates supplemented with second‐messenger effectors

et al. 1999

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“…The present study was undertaken to investigate the benefits of adding prostaglandin El (PGEJ (56 nM final concentration) in vitro during routine PC preparation. Early studies showed that the addition of low concentrations of PGEl to whole blood facilitated the separation, concentration and resuspension of platelets (Becker et al, 1972(Becker et al, , 1974Shio & Ramwell, 1972;Valeri et al, 1972). In addition, Menitove et al (1986) demonstrated that the P-thromboglobulin (P-TG) release and thromboxane B2 (TxB2) production associated with the preparation and storage of PCs was significantly reduced when PGEI was added to PRP prior to centrifugation.…”

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Use of prostaglandin E₁ during preparation of platelet concentrates

Hawker¹,

Turner²,

Mitchell³

1996

Transfusion Medicine

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A paired study in 10 autologous volunteer donors was undertaken to investigate the efficacy of adding prostaglandin E1 (PGE1) in vitro during routine platelet concentrate (PC) production. After 5 days storage, PCs prepared with PGE1 were compared with control PCs. In vivo platelet recovery, survival and biodistribution were determined following autologous infusion of indium-111 labelled platelets into volunteers, together with the in vitro evaluation of platelet function and biochemistry. PGE1 facilitated easier and faster platelet resuspension following centrifugation. After storage there were few significant in vitro differences between PCs prepared with PGE1 and control PCs. The artifactual leucocyte concentration was significantly lower in the presence of PGE1, suggesting less platelet aggregates had been formed during storage and beta-thromboglobulin release was significantly reduced by PGE1, 14.0 +/- 6.0 micrograms per 10(9) platelets compared with 22.3 +/- 9.8 micrograms per 10(9) platelets in control PCs, (P < 0.01), indicating PGE1 reduced both platelet aggregation and activation probably at the initial preparation stage, known to produce the greatest trauma. Initial in vivo platelet recovery for PCs prepared with PGE1 was similar to that of control PCs, 41.1 +/- 12.5% vs. 44.4 +/- 8.0%, respectively, and there were no differences in organ distribution at 24 h. However, in vivo multiple hit survival was reduced in the presence of PGE1, 5.8 +/- 1.6 days compared with 6.9 +/- 1.4 days in control PCs (P < 0.05). Despite the ability of PGE1 to facilitate platelet resuspension and inhibit platelet aggregation and activation during preparation of the PCs, the reduced in vivo survival time may preclude the use of PGE1 during routine PC preparation.

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Prostaglandin E ₁ in Preparation and Storage of Platelet Concentrates

Abstract: The addition of prostaglandin E(1) to blood collection bags improved platelet harvesting; platelets were, esily suspended immediately after centrifugation. The treatment with prostaglandin E(1) did not affect the survival time of these platelets after infusion into a recipient.

Cited by 28 publications

References 4 publications

Effects of Prostaglandin E₁on the Preparation of Platelet Concentrates in Dogs

Effects of Prostaglandin E₁on the Preparation of Platelet Concentrates in Dogs

Quality assessment of platelet concentrates supplemented with second‐messenger effectors

Use of prostaglandin E₁ during preparation of platelet concentrates

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Prostaglandin E 1 in Preparation and Storage of Platelet Concentrates

Abstract: The addition of prostaglandin E(1) to blood collection bags improved platelet harvesting; platelets were, esily suspended immediately after centrifugation. The treatment with prostaglandin E(1) did not affect the survival time of these platelets after infusion into a recipient.

Cited by 28 publications

References 4 publications

Effects of Prostaglandin E1on the Preparation of Platelet Concentrates in Dogs

Effects of Prostaglandin E1on the Preparation of Platelet Concentrates in Dogs

Quality assessment of platelet concentrates supplemented with second‐messenger effectors

Use of prostaglandin E1 during preparation of platelet concentrates

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Product

Resources

About

Prostaglandin E ₁ in Preparation and Storage of Platelet Concentrates

Effects of Prostaglandin E₁on the Preparation of Platelet Concentrates in Dogs

Effects of Prostaglandin E₁on the Preparation of Platelet Concentrates in Dogs

Use of prostaglandin E₁ during preparation of platelet concentrates