Effects of Prostaglandin E₁on the Preparation of Platelet Concentrates in Dogs

Abstract

Background: Platelet concentrates (PC) are prepared by centrifugation of platelet-rich plasma (PRP) that is prepared by centrifugation of whole blood. The resuspension of the platelet pellet during PC preparation from dogs is difficult because of platelet activation induced by centrifugation.Objectives: To investigate the efficacy of adding prostaglandin E 1 (PGE 1 ) to prevent platelet activation during PC preparation from dogs.Animals: Fifteen healthy Beagle dogs. Methods: Prospective, experimental trial: PGE 1 was added to PRP before the high-speed centrifugation during PC preparation. To estimate the effect of this addition, we assessed the platelet aggregability before transfusion, the survival of the platelets after transfusion, and the platelet reactivity after transfusion, which is estimated by the P-selectin expression of the platelets when stimulated by thrombin.Results: The difficulty associated with platelet resuspension was resolved by PGE 1. PGE 1 strongly inhibited platelet aggregation induced by collagen and ADP; however, it recovered after the platelets were resuspended in plasma without PGE 1 (mean aggregation ratio; collagen: 10.00-80.80%, ADP: 8.20-53.60%). Survival of the platelets after transfusion was not affected by PGE 1 (mean 8.04 and 7.56 days, without and with PGE 1 ), and thrombin-induced P-selectin expression after transfusion in PGE 1 -treated PC was also well maintained (mean positive ratio 53.7 and 47.9%, before and 24 hours after transfusion).Conclusions and Clinical Importance: The addition of PGE 1 in PRP before the centrifugation of PRP can improve the preparation efficiency of PC from dogs, while maintaining the therapeutic efficacy of the platelets.

Description of a double centrifugation tube method for concentrating canine platelets

“…The platelet activation in fact can contribute to the development of platelet storage lesions and decrease platelet viability. It is therefore important to develop a method to minimize platelet activation during the preparation of platelet concentrates [19]. As described by Segawa in 2012 the addition of prostaglandin E 1 (PGE1) in PRP before the centrifugation of PRP can improve the preparation efficiency of platelet concentrates from dogs, while maintaining the therapeutic efficacy of the platelets [19].…”

“…It is therefore important to develop a method to minimize platelet activation during the preparation of platelet concentrates [19]. As described by Segawa in 2012 the addition of prostaglandin E 1 (PGE1) in PRP before the centrifugation of PRP can improve the preparation efficiency of platelet concentrates from dogs, while maintaining the therapeutic efficacy of the platelets [19]. It would be interesting to evaluate the method here described in another group of dogs studying whether the addition of PGE improve the yield and effectiveness of platelets reducing the percentage of activation.…”

Platelet Proteome Changes in Dogs with Chronic Heart Failure

“…HEPES buffer solution (137 mM NaCl, 3.8 mM HEPES, 5.6 mM Glucose, 2.7 mM KCl, 1 mM Magnesium sulfate, pH 7.4; Sigma) was added over the PRP samples. This was suspended with prostaglandin E1 (1 µM/L, Sigma) to inhibit platelet activation during high-speed centrifugation (58). This mix was centrifuged at 800 g for 15 min to concentrate the platelets.…”

Platelet proteome changes in dogs with congestive heart failure

“…HEPES buffer solution (137 mM NaCl, 3.8 mM HEPES, 5.6 mM Glucose, 2.7 mM KCl, 1 mM Magnesium sulfate, pH 7.4; Sigma) was added over the PRP samples. This was suspended with prostaglandin E1 (1 mM/L, Sigma) to inhibit platelet activation during high-speed centrifugation (75). This mix was centrifuged at 800 g for 15 min to concentrate the platelets.…”