2013
DOI: 10.1186/1746-6148-9-146
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Abstract: BackgroundTo evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was processed by a double cen… Show more

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Cited by 23 publications
(25 citation statements)
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References 20 publications
(44 reference statements)
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“…Accomplishing this may require the use of a double centrifugation approach, which allows a reduction in the WBC concentration [340]. The concentration of platelets in PRP varies by four-to ninefold depending on the separation system used [328,336,341].…”
Section: Cautions In Using Prp: Prp Compositionmentioning
confidence: 99%
See 1 more Smart Citation
“…Accomplishing this may require the use of a double centrifugation approach, which allows a reduction in the WBC concentration [340]. The concentration of platelets in PRP varies by four-to ninefold depending on the separation system used [328,336,341].…”
Section: Cautions In Using Prp: Prp Compositionmentioning
confidence: 99%
“…Some methods can yield relatively consistent PRP compositions, such as a double centrifugation tubes allow the consistent separation of dog PRP at about four times the base level and WBCs at not more than twofold above base level [340].…”
Section: Cautions In Using Prp: Prp Compositionmentioning
confidence: 99%
“…Compared to whole blood platelet concentrates obtained from equine donors and processed by a double centrifugation tube method contained 1,21 to 1,7 fold higher platelet concentrations [15]. However PRP obtained from canine donors and processed by a double centrifugation tube method revealed platelet concentrations up to 4.9 fold higher than baseline values [16]. As far as growth factor content of the completed PRP-preparation is considered, critical for the intended enhancement of wound healing and regenerative capacities at site of the lesion, final concentrations at time of application are of particular interest.…”
Section: Introductionmentioning
confidence: 99%
“…The platelet accumulates at the bottom of the tube, the PPP on top. The PPP is drawn off so that the PRP remains in the tube; the final PRP was obtained approximately 1 mL drawn up with an insulin syringe into Petri dish and platelets were activated by adding 0.2 mL of 10% calcium chloride to form PRP gel which was used in the wounded area [17].…”
Section: Preparation Of Prpmentioning
confidence: 99%