Prostacyclin secretion by human endothelial cells grown on carbodiimide cross-linked proteins: An assessment of the cytocompatibility of a substratum

Penhoat, J.; Sigot‐Luizard, M. F.; Warocquier-Clerout, R.

doi:10.1016/0142-9612(93)90237-v

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…This effect was most pronounced for tPA and vWF, the release of tPA and vWF decreased sharply when the number of cells increased. At cell numbers higher than 10,000-20,000 cells/cm 2 the release was almost independent of cell density, which confirms observations by others (35,36).…”

Section: Discussionsupporting

confidence: 90%

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Wissink

Luyn

Beernink³

et al. 2000

Thromb Haemost

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SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

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Section: Discussionsupporting

confidence: 90%

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Wissink

Luyn

Beernink³

et al. 2000

Thromb Haemost

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“…vWF secretion was found to be low in non-stimulated HUVEC on TCPS [140] and on fibronectin coated polystyrene sodium sulfonate microcarriers [141]. Similarly PGI 2 secretion, measured using immuno-or radio-immuno-assays, was low in nonstimulated HUVEC and BAEC cultured on plastic microspheres (Biosilon®) and dishes [142,143] and carbodiimide crosslinked gelatin and albumin gels [144]. HUVEC, analyzed by flow cytometry after removal from TCPS, Dacron, and Teflon showed no E-selectin and low levels of VCAM-1, independent of substrate, and HOTMEC showed no VCAM-1 and low levels of E-selectin.…”

Section: Molecular Expression Studiesmentioning

confidence: 96%

“…EC function has also been shown to depend on cell density and level of confluence [140]. A number of studies have noted significant differences in initial EC function dependent on the underlying substrate, which become insignificant when confluence is reached [140,144,164,165]. For example, bovine and porcine EC cultured sparsely on TCPS, coated with different concentrations of poly(HEMA) to vary adhesiveness, showed variations in F-actin and cell shape which correlated with levels of prostacyclin [166].…”

Section: Molecular Expression Studiesmentioning

confidence: 99%

The influence of biomaterials on endothelial cell thrombogenicity

2007

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Driven by tissue engineering and regenerative medicine, endothelial cells are being used in combination with biomaterials in a number of applications for the purpose of improving blood compatibility and host integration. Endothelialized vascular grafts are beginning to be used clinically with some success in some centers, while endothelial seeding is being explored as a means of creating a vasculature within engineered tissues. The underlying assumption of this strategy is that when cultured on artificial biomaterials, a confluent layer of endothelial cells maintain their nonthrombogenic phenotype. In this review the existing knowledge base of endothelial cell thrombogenicity cultured on a number of different biomaterials is summarized. The importance of selecting appropriate endpoint measures that are most reflective of overall surface thrombogenicity is the focus of this review. Endothelial cells inhibit thrombosis through three interconnected regulatory systems (1) the coagulation cascade (2) the cellular components of the blood such as leukocytes and platelets and (3) the complement cascade, and also through effects on fibrinolysis and vascular tone, the latter which influences blood flow. Thus, in order to demonstrate the thromobgenic benefit of seeding a biomaterial with EC, the conditions under which EC surfaces are more likely to exhibit lower thrombogenicity than unseeded biomaterial surfaces need to be consistent with the experimental context. The endpoints selected should be appropriate for the dominant thrombotic process that occurs under the given experimental conditions.

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“…18 Briefly, albumin and gelatin were dissolved 20% and 10%, respectively in PBS, pH 4.75. Gelatin (G) and albumin-gelatin (AG) gels were obtained by pouring 0.2 mL of gelatin or 0.2 mL of a mixture of albumin and gelatin (1:2) in four-well plates (Nunclon).…”

Section: Protein Coating Of Growth Surfacementioning

confidence: 99%

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Laemmel

Penhoat

Warocquier-Clerout

et al. 1998

J. Biomed. Mater. Res.

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Gelatin or a mixture of albumin and gelatin has been proposed for the coating of vascular grafts according to their surface thrombogenicity and biocompatibility, and the possibility of biodegradation. Heparin treatment of hemocompatible surfaces improved the patency of prostheses. In this study, different amounts of heparin were immobilized on these protein gels using a water-soluble carbodiimide [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide]. The results showed a coupling of heparin with gelatin and/or albumin at the surface of the gels, stable for as long as 1 month. From 0.20 to 3.60 microg x cm(-2), heparin could be immobilized. The antiproliferative activity of immobilized heparin was controlled toward bovine smooth-muscle cells grown on these gels. Cell growth inhibition was dose dependent, but the percentages of inhibition were lower at day 8 than at day 4 at any heparin concentration used under experimental conditions. Referring to heparin in solution, immobilized heparin displayed an antiproliferative activity that improved the potential interest for coating.

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Prostacyclin secretion by human endothelial cells grown on carbodiimide cross-linked proteins: An assessment of the cytocompatibility of a substratum

Cited by 8 publications

References 13 publications

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

The influence of biomaterials on endothelial cell thrombogenicity

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

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