Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Abstract

Gelatin or a mixture of albumin and gelatin has been proposed for the coating of vascular grafts according to their surface thrombogenicity and biocompatibility, and the possibility of biodegradation. Heparin treatment of hemocompatible surfaces improved the patency of prostheses. In this study, different amounts of heparin were immobilized on these protein gels using a water-soluble carbodiimide [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide]. The results showed a coupling of heparin with gelatin and/or albumin at the surface of the gels, stable for as long as 1 month. From 0.20 to 3.60 microg x cm(-2), heparin could be immobilized. The antiproliferative activity of immobilized heparin was controlled toward bovine smooth-muscle cells grown on these gels. Cell growth inhibition was dose dependent, but the percentages of inhibition were lower at day 8 than at day 4 at any heparin concentration used under experimental conditions. Referring to heparin in solution, immobilized heparin displayed an antiproliferative activity that improved the potential interest for coating.

The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization

“…In theory, a sustained released of antirestenotic drugs for at least three weeks is required to prevent SMC migration and proliferation [49]. Heparin was also proven to have the ability to inhibit SMC proliferation [50]. In our study, a confluent coverage layer of ECs formed at the 5th day culture during which time heparin still kept anticoagulation properties.…”

Coimmobilization of heparin/fibronectin mixture on titanium surfaces and their blood compatibility

“…Additionally, Laemmel et al reported that immobilization of heparin may prevent intimal hyperplasia, which often leads to graft failure [34]. Heparin mainly performs its anticoagulant properties by binding to AT III and indirectly impacting the intrinsic coagulation pathway (APTT), however, anticoagulant activity of the immobilized heparin depends on different reaction conditions.…”

Materials for Peripheral Nerve Regeneration

“…Gelatin chain backbones contain primary carboxyl groups which may bind the amine groups of neurotrophic molecules 57–59. Nerve regeneration has been found to be enhanced by filling nerve guides with neurotrophic factors, such as nerve growth factor (NGF), glial cell line‐derived neurotrophic factor (GDNF) and neurotrophin NT‐3 60,61.…”