Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances

Maina, Sofia; Prabhu, Ashish A.; Narisetty, Vivek; Vlysidis, Anestis; Koutinas, Apostolis; Kumar, Vinod

doi:10.1016/j.biotechadv.2021.107783

Cited by 74 publications

(51 citation statements)

References 239 publications

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“…case of arabinose [11,20]. Biological production of bulk chemicals has garnered increasing attention in recent years and one example is the microbial production of BDO [12]. To date little information is available on the biosynthesis of BDO from arabinose and in the current work, we have investigated BDO production using pure arabinose and crude arabinose from SBP by E. ludwigii.…”

Section: Discussionmentioning

confidence: 99%

Biological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigii

Narisetty

Jacob

et al. 2022

Renewable Energy

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Section: Discussionmentioning

confidence: 99%

Biological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigii

Narisetty

Jacob

et al. 2022

Renewable Energy

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“…Firstly, 2,3-butanediol (2,3-BDO) is a valuable chemical feedstock due to its potential applications, including its use as a liquid fuel and for the production of various chemicals, foods, and pharmaceuticals [ 1 , 2 , 3 , 4 ]. The global market of the BDO is expected to reach a value of USD 220 million by 2027, growing at a compound annual growth rate (CAGR) of 3% from 2019 to 2027 [ 5 ], and the current market price of 2,3-BDO is higher than that of 1,4-butanediol [ 6 ]. The production of 2,3-BDO through a bioprocess is an attractive aim, given its environmental friendliness.…”

Section: Introductionmentioning

confidence: 99%

“…For 2,3-BDO production, the hexoses enter the microbial metabolism through PTS (phosphortransferase system) and convert into 2 moles of pyruvate via the Embden–Meyerhof pathway (glycolysis) with the generation of 2 moles of NADH and 2 moles of ATP. Pyruvate is then channeled for the generation of metabolites such as acetate, formate, lactate, succinate, ethanol, and acetoin, besides BDO [ 5 ]. Thus far, little is known about the functional aspect of the metabolic regulation of 2,3-BDO production.…”

Section: Introductionmentioning

confidence: 99%

“…In shaken flasks, approximately 14.5, 16.8, and 8.0 g/L 2,3-BDO was produced by P. polymyxa [ 21 ], Bacillus amyloliquefaciens B10-127 [ 22 ], and Raoultella terrigena CECT 4519 [ 23 ], respectively. Among the strains, 2,3-BDO metabolism is dependent on strict oxygen control to ensure an oxygen supply condition, and oxygen supply is considered one of the most critical factors in 2,3-BDO production, affecting the distribution of metabolites produced, yield, and productivity [ 5 , 10 ]. However, oxygen supply is also a high-cost process that requires the supply of compressed air or pure oxygen.…”

Section: Introductionmentioning

confidence: 99%

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Effectively Converting Cane Molasses into 2,3-Butanediol Using Clostridiumljungdahlii by an Integrated Fermentation and Membrane Separation Process

et al. 2022

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Firstly, 2,3-butanediol (2,3-BDO) is a chemical platform used in several applications. However, the pathogenic nature of its producers and the expensive feedstocks used limit its scale production. In this study, cane molasses was used for 2,3-BDO production by a nonpathogenic Clostridium ljungdahlii. It was found that cane molasses alone, without the addition of other ingredients, was favorable for use as the culture medium for 2,3-BDO production. Compared with the control (i.e., the modified DSMZ 879 medium), the differential genes are mainly involved in the pathways of carbohydrate metabolism, membrane transport, and amino acid metabolism in the case of the cane molasses alone. However, when cane molasses alone was used, cell growth was significantly inhibited by KCl in cane molasses. Similarly, a high concentration of sugars (i.e., above 35 g/L) can inhibit cell growth and 2,3-BDO production. More seriously, 2,3-BDO production was inhibited by itself. As a result, cane molasses alone with an initial 35 g/L total sugars was suitable for 2,3-BDO production in batch culture. Finally, an integrated fermentation and membrane separation process was developed to maintain high 2,3-BDO productivity of 0.46 g·L−1·h−1. Meanwhile, the varied fouling mechanism indicated that the fermentation properties changed significantly, especially for the cell properties. Therefore, the integrated fermentation and membrane separation process was favorable for 2,3-BDO production by C. ljungdahlii using cane molasses.

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“…On the other hand, acetoin, the precursor of or product from 2,3-BD, can be oxidized to diacetyl, which is used as a high-value flavoring of food that gives food a buttery taste and as a bacteriostatic additive for food processing [3,9]. In the global market, 2,3-BD is expected to grow at a compound annual growth rate of 3% from 2019 to 2027, reaching US $ 220 million by 2027, and the current market price of 2,3-BD is higher than that of 1,4-butanediol [6,11,12]. The downstream products of BD have the potential of a global market of around 32 million tons per annually, worth US $ 43 billion [7,8].…”

Section: Introductionmentioning

confidence: 99%

Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii

et al. 2022

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Background 2,3-Butanediol (2,3-BD), a valuable compound used for chemicals, cosmetics, pesticides and pharmaceuticals, has been produced by various microbes. However, no high-temperature fermentation of the compound at high productivity has been reported. Methods Thermotolerant xylose-utilizing microbes were isolated from 6 different districts in Laos and screened for a low accumulation of xylitol in a xylose medium at 37 ˚C. One isolate was found to produce 2,3-BD and identified by 16S rDNA sequencing. The 2,3-BD fermentation capacity was investigated at different temperatures using xylose and glucose as carbon sources, and the fermentation parameters were determined by a high-performance liquid chromatography system. Results By screening for a low accumulation of xylitol in a xylose medium, one isolate that accumulated almost no xylitol was obtained. Further analyses revealed that the isolate is Cronobacter sakazakii and that it has the ability to produce 2,3-BD at high temperatures. When xylose and glucose were used, this strain, named C. sakazakii OX-25, accumulated 2,3-BD in a short period before the complete consumption of these sugars and then appeared to convert 2,3-BD to acetoin. The optimum temperature of the 2,3-BD fermentation was 42 ˚C to 45 ˚C, and the maximum yield of 2,3-BD was 0.3 g/g at 12 h in 20 g/l xylose medium and 0.4 g/g at 6 h in 20 g/l glucose medium at 42 ˚C. The 2,3-BD productivity of the strain was higher than the 2,3-BD productivities of other non-genetically engineered microorganisms reported previously, and the highest productivity was 0.6 g/l·h and 1.2 g/l·h for xylose and glucose, respectively. Conclusions Among thermotolerant microbes isolated in Laos, we discovered a strain, C. sakazakii OX-25, that can convert xylose and glucose to 2,3-BD with high efficiency and high productivity at high temperatures, suggesting that C. sakazakii OX-25 has the potential for industrial application to produce 2,3-BD as an important platform chemical.

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Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances

Cited by 74 publications

References 239 publications

Biological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigii

Biological production and recovery of 2,3-butanediol using arabinose from sugar beet pulp by Enterobacter ludwigii

Effectively Converting Cane Molasses into 2,3-Butanediol Using Clostridiumljungdahlii by an Integrated Fermentation and Membrane Separation Process

Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii

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