Prospects of curcumin as an additive in storage solutions: a study on erythrocytes

Ravikumar, S.; Hsieh, Carl; Vani, R.

doi:10.3906/sag-1501-93

Cited by 4 publications

(5 citation statements)

References 66 publications

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“…Various studies have been performed on stored blood to decrease the observed negative changes in a time-dependent manner and to increase the lifespan of RBCs. 5 , 6 , 32 , 33 …”

Section: Discussionmentioning

confidence: 99%

“…performed on stored blood to decrease the observed negative changes in a timedependent manner and to increase the lifespan of RBCs. 5,6,32,33 Dumaswala et al 34 showed that an increasing concentration of GSH in stored blood protected RBCs against free radical damage. Additionally, numerous studies have shown that lipid peroxidation is attenuated by the addition of metal chelators and antioxidant substances to stored blood or RBCs.…”

Section: Discussionmentioning

confidence: 99%

“…This is because reactive oxygen species (ROS), such as hydroxyl radical (OH − ), superoxide anion radical ( 1 O 2 − ), and hydrogen peroxide (H 2 O 2 ), increase oxidative stress in a time-dependent manner. 10 , 13 ROS induce oxidation in membrane lipids and proteins, and antioxidants in the phenolic structure can scavenge the factors that cause oxidative stress. 14 , 15 Therefore, the lifespan of stored RBCs can be extended by reducing oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner

et al. 2017

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ObjectiveIn stored red blood cells (RBCs), which are used in diseases (e.g., acute blood loss and leukaemia), storage lesions arise by oxidative stress and other factors over time. This study investigated the protective effects of resveratrol and serotonin on stored RBCs.MethodsBlood from each donor (n = 10) was placed in different bags containing 70 mL of citrate phosphate dextrose (total volume: 500 mL) and divided into three groups (n = 30): control, 60 µg/mL resveratrol, and 60 µg/mL serotonin. Malondialdehyde (MDA) and glutathione (GSH) levels, activity of glutathione peroxidase (GSH-Px), catalase, and carbonic anhydrase (CA), and susceptibility to oxidation in RBCs, and pH in whole blood were measured at baseline and on days 7, 14, 21, and 28.ResultsMDA levels and susceptibility to oxidation were increased in all three groups time-dependently, but this increase was greater in the serotonin group than in the other groups. Activity of GSH-Px, CAT, and CA, as well as GSH levels, were decreased in the control and serotonin groups time-dependently, but were significantly preserved in the resveratrol group. The pH was decreased in all groups time-dependently.ConclusionOur study shows that resveratrol attenuates susceptibility to oxidation of RBCs and protects their antioxidant capacity, and partially preserves CA activity time-dependently.

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“…Various studies have been performed on stored blood to decrease the observed negative changes in a time-dependent manner and to increase the lifespan of RBCs. 5 , 6 , 32 , 33 …”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner

et al. 2017

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“…An antioxidant is defined as "any substance that, when present at low concentrations compared to those of an oxidizable substrate, significantly delays or prevents oxidation of that substrate" [11]. Various studies have reported the beneficial effects of antioxidants (L-carnitine [LC], curcumin [Cu], vitamin C [VC]) in blood storage solutions [12][13][14][15][16][17][18][19][20][21][22].…”

Section: L-carnitinementioning

confidence: 99%

“…Cu increases intracellular glutathione (GSH) and regulates antioxidant enzymes. It also protects oxyhemoglobin from nitrite-induced oxidation [12,22].…”

Section: L-carnitinementioning

confidence: 99%

Ferric reducing ability of plasma: a potential oxidative stress marker in stored plasma

Hsieh

Vani

2021

Acta Haematol Pol.

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Introduction:The ferric reducing ability of plasma (FRAP) assay is used for measuring the antioxidant capacity. FRAP is proportional to the molar concentration of the antioxidant capacity. This study attempts to analyze the possibilities of FRAP as an indicator of oxidative stress.Methods: Blood was drawn from male Wistar rats and stored for 20 days at 4°C in citrate phosphate dextrose adenine 1. The rats were divided into two groups: controls and experimentals. The experimentals were added with antioxidants -L-carnitine, curcumin, vitamin C (VC), and caffeic acid of varying concentrations -10, 30, and 60 mM (n =5 for each group). Plasma was isolated from these samples at regular intervals (every 5 days), and FRAP and protein were assayed. Results were analyzed by two-way ANOVA, using GraphPad prism 6. FRAP was maintained in controls.Results: VC (ascorbic acid) was the most potent antioxidant in terms of FRAP during storage compared with the above antioxidants. This study emphasizes the use of FRAP as a potential marker of oxidative stress in plasma of stored blood. Conclusion:FRAP can be utilized as a reliable marker for determining the antioxidant capacity.

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Age-Related Modulations in Erythrocytes under Blood Bank Conditions

Hsieh

Ncs

Rajashekaraiah

2019

Transfus Med Hemother

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Background: During storage of erythrocytes, storage lesions are formed that reduce the safety and efficacy of the stored blood. Thus, there is a need to understand the changes that occur during storage. Most studies have focused on storage of a mixed population of erythrocytes. The aim of this study is to analyze the changes in young and old erythrocytes over the course if storage. Materials and Methods: Blood was collected from the blood bank at the Kempegowda Institute of Medical Sciences (KIMS) Hospital (Bengaluru, India) and stored for 35 days in CPDA-1 at 4°C. Every 5 days, erythrocytes were separated based on the blood’s age using a Percoll-BSA gradient. Young and old erythrocytes obtained were used for analysis of the following oxidative stress (OS) markers: hemoglobin (Hb), hemolysis, mechanical fragility, antioxidant enzymes (superoxide dismutase and catalase [CAT]), superoxides, sialic acid, glutamic oxaloacetate transaminase (GOT), glucose, plasma membrane redox system (PMRS), total antioxidant capacity-cupric ion reducing antioxidant capacity assay (TACCUPRAC), lactate dehydrogenase (LDH), lipid peroxidation products (malondialdehyde [MDA] and conjugate dienes), and protein oxidation products (advanced oxidation protein products and protein sulfhydryls). Result: Young cells had higher amounts of Hb, sialic acid, GOT, LDH, TACCUPRAC, CAT, and superoxides compared to old cells. Old cells, however, had higher PMRS and MDA levels with respect to young cells. Discussion: Young cells could endure OS during storage more efficiently than old cells. In conclusion, the efficacy of stored blood depends on the ratio of young cells in the population. This study highlights the prospects of storing young erythrocytes for a prolonged shelf life of blood.

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Prospects of curcumin as an additive in storage solutions: a study on erythrocytes

Cited by 4 publications

References 66 publications

Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner

Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner

Ferric reducing ability of plasma: a potential oxidative stress marker in stored plasma

Age-Related Modulations in Erythrocytes under Blood Bank Conditions

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