Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner

Huyut, Zübeyir; Şekeroğlu, Mehmet Ramazan; Balahoroğlu, Ragıp; Huyut, Mehmet Tahir

doi:10.1177/0300060517725450

Cited by 13 publications

(9 citation statements)

References 41 publications

(90 reference statements)

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“…Oxidative stress can be defined as ‘ a disturbance in the prooxidant‐antioxidant balance, in favor of the former, leading to potential damage ’ (Halliwell & Whiteman, ). Erythrocytes are highly prone to oxidative damage because of their constant presence in an environment of oxygen (Huyut et al ., ). Haemoglobin is abundantly present in erythrocytes, and the autoxidation of haemoglobin produces superoxide radicals, which initiate the formation of different reactive oxygen species (ROS) (D'Alessandro et al ., ).…”

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confidence: 97%

Effects of L‐carnitine on the erythrocytes of stored human blood

Ravikumar

Prabhu

Vani

2019

Transfusion Medicine

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SUMMARY Objectives This study aimed to assess the effects of L‐carnitine on oxidative stress in human erythrocytes during storage. Background Using antioxidants as components of blood storage solutions may combat the effects of storage‐induced oxidative stress on erythrocytes. Methods Blood from male adults was stored at 4 °C for 55 days in citrate phosphate dextrose adenine solution, without L‐carnitine (Control) and with L‐carnitine as an additive (at concentrations of 10, 30 and 60 mM – Experiments). Every fifth day, erythrocyte markers (morphology, count, haemoglobin, haemolysis and osmotic fragility), antioxidant defences (antioxidant enzymes and total antioxidant capacity) and oxidative stress markers (superoxides, lipid peroxidation and protein oxidation products) were analysed. Results Oxidative damage was observed in controls (day 25 onwards) and in experiments (day 35 onwards). L‐carnitine (10 and 30 mM) protected erythrocytes from damage up to day 35 by maintaining haemoglobin and lipid peroxidation, assisting antioxidant enzymes and increasing antioxidant capacity by elevating sulfhydryls and ascorbic acid. L‐carnitine was beneficial in prolonging storage up to 55 days but could not prevent oxidative damage completely in terms of haemolysis and osmotic fragility. Conclusions L‐carnitine ameliorated oxidative stress, but combinations with other antioxidants may provide comprehensive protection to erythrocytes during storage.

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confidence: 97%

Effects of L‐carnitine on the erythrocytes of stored human blood

Ravikumar

Prabhu

Vani

2019

Transfusion Medicine

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“…ROS causes lipid, protein and DNA oxidation, which are structural elements in cells (31,32). Upon lipid and protein oxidation in the cell membrane, the cells become susceptible for penetration and their integrity disrupted.…”

Section: Discussionmentioning

confidence: 99%

Actions of resveratrol and serotonin added to stored bloods on lipid peroxidation and oxidative DNA damage

Huyut¹,

Şekeroğlu²,

Hakan³

et al. 2018

Eastern J Med

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“…Then calibration curve was obtained with different standard concentrations by using Pb cathode lamp (Hollow Cathode Lamp, Hamamatsu Photonics K.K Made in Japan) and measurement was performed at 283.3 nm with atomic absorption combustion unit (Graphite Furnace Atomizer, GFA-7000 Shimadzu). In addition, hemoglobin was measured according to method used by Huyut et al (20). Hemolyzate (20 µL) was added onto 5 mL drabkin solution and incubated for 10 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%