Prospects for the use of animal cell cultures in screening of pharmaceutical substances

Kolesnikova, S G; Moiseeva, I. Ya.

doi:10.1088/1742-6596/784/1/012028

Cited by 3 publications

(2 citation statements)

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“…The safety of drug therapy is one of the most important tasks of modern health care. Recently, animal cell cultures are increasingly found in toxicological studies methods as an alternative to the classical experimental animal tests [35]. Additionally, these methods can significantly solve the ethical problems associated with the mass use and death of experimental animals, and reduce the cost and time of preliminary studies of new chemical drugs mainly at the stage of pre-clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Ellagic acid microspheres restrict the growth of Babesia and Theileria in vitro and Babesia microti in vivo

2019

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Background There are no effective vaccines against Babesia and Theileria parasites; therefore, therapy depends heavily on antiprotozoal drugs. Treatment options for piroplasmosis are limited; thus, the need for new antiprotozoal agents is becoming increasingly urgent. Ellagic acid (EA) is a polyphenol found in various plant products and has antioxidant, antibacterial and effective antimalarial activity in vitro and in vivo without toxicity. The present study documents the efficacy of EA and EA-loaded nanoparticles (EA-NPs) on the growth of Babesia and Theileria . Methods In this study, the inhibitory effect of EA, β-cyclodextrin ellagic acid (β-CD EA) and antisolvent precipitation with a syringe pump prepared ellagic acid (APSP EA) was evaluated on four Babesia species and Theileria equi in vitro , and on the multiplication of B. microti in mice. The cytotoxicity assay was tested on Madin-Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3T3) and human foreskin fibroblast (HFF) cell lines. Results The half-maximal inhibitory concentration (IC 50 ) values of EA and β-CD EA on B. bovis , B. bigemina , B. divergens , B. caballi and T. equi were 9.58 ± 1.47, 7.87 ± 5.8, 5.41 ± 2.8, 3.29 ± 0.42 and 7.46 ± 0.6 µM and 8.8 ± 0.53, 18.9 ± 0.025, 11 ± 0.37, 4.4 ± 0.6 and 9.1 ± 1.72 µM, respectively. The IC 50 values of APSP EA on B. bovis , B. bigemina , B. divergens , B. caballi and T. equi were 4.2 ± 0.42, 9.6 ± 0.6, 2.6 ± 1.47, 0.92 ± 5.8 and 7.3 ± 0.54 µM, respectively. A toxicity assay showed that EA, β-CD EA and APSP EA affected the viability of cells with a half-maximal effective concentration (EC 50 ) higher than 800 µM. In the experiments on mice, APSP EA at a concentration of 70 mg/kg reduced the peak parasitemia of B . microti by 68.1%. Furthermore, the APSP EA-atovaquone (AQ) combination showed a higher chemotherapeutic effect than that of APSP EA monotherapy. Conclusions To our knowledge, this is the first study to demonstrate the in vitro and in vivo antibabesial action of EA-NPs and thus supports the use of nanoparticles as an alternative antiparasitic agent. Electronic supplementary material The online version of this article (10.1186/s13071-019-3520-x) contains supplementary ma...

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Section: Discussionmentioning

confidence: 99%

Ellagic acid microspheres restrict the growth of Babesia and Theileria in vitro and Babesia microti in vivo

2019

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“…Cell culture is a versatile tool for modeling cell behavior, cell interaction and disease states. Cell culture models are used to study drug effects and toxicity in vitro, as they mimic human physiology during preclinical screening, providing efficacy and risk assessment data [8][9][10]. There is a diverse set of methods to culture human cells, namely 2D cultures, organs-on-a-chip, tissue engineering, etc.…”

Section: Introductionmentioning

confidence: 99%

Track‐Etched Membranes for Drug Pharmaceutical Research

Cuanalo-Contreras,

Hogrebe,

Teichmann

et al. 2023

Chemie Ingenieur Technik

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Over the last decades, the incorporation of membranes into micro physiological systems used for pharmaceutical research and drug development has grown significantly. One example is the use of microporous track‐etched membranes in microfluidic systems like organs‐on‐a‐chip and cell culture inserts for tissue engineering. Tissue‐culture treated track‐etched membranes serve as excellent support for cell growth and efficient nutrient supply, with the added benefit of precise control of pore parameters, a critical pre‐requisite to achieve reproducible results. Here, we provide an overview of track‐etched membrane technology in the context of applications in drug discovery and development.

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Electropermeabilization of metastatic chondrosarcoma cells from primary cell culture

Michel

Błasiak

Saczko

et al. 2019

Biotech and App Biochem

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Primary cell cultures are challenging, but reliable model reflecting tumor response in vitro. The study was designed to examine if the increased electropermeabilization can overcame initial drug insensitivity in chondrosarcoma cells from lung metastasis. We established a primary cell culture and evaluated the cytotoxic impact of four drugs—cisplatin (CDDP), camptothecin, 2‐methoxyestradiol, and leucovorin calcium (LeuCa). After determination of parameters allowing for electropermeabilization, we performed electrochemotherapy in vitro with the least toxic drugs—CDDP and LeuCa. Although combining CDDP and leucovorin together increased their toxicity and supported apoptosis, application of pulsed electric fields (PEFs) brought no advantage for their efficacy. The study emphasizes the need for introduction of primary cell cultures into studies on pulse electric fields as model frequently less sensitive to PEF‐based treatments than continuous cell lines.

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Prospects for the use of animal cell cultures in screening of pharmaceutical substances

Cited by 3 publications

References 1 publication

Ellagic acid microspheres restrict the growth of Babesia and Theileria in vitro and Babesia microti in vivo

Ellagic acid microspheres restrict the growth of Babesia and Theileria in vitro and Babesia microti in vivo

Track‐Etched Membranes for Drug Pharmaceutical Research

Electropermeabilization of metastatic chondrosarcoma cells from primary cell culture

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