Prospects and challenges in engineering functional respiratory epithelium for in vitro and in vivo applications

Kumar, Pramod; Vrana, Nihal Engin; Ghaemmaghami, Amir M.

doi:10.21037/mps.2017.09.01

Cited by 7 publications

(8 citation statements)

References 71 publications

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“…Collagen and hyaluronan are two commonly used natural biomaterials for the ciliary differentiation of human respiratory epithelial cells [72]. In combination, bi-layered collagen-hyaluronate scaffolds have been shown to facilitate lung epithelial cell differentiation and mucin expression [73].…”

Section: Tissue Scaffoldsmentioning

confidence: 99%

Current and Alternative Therapies for Nasal Mucosa Injury: A Review

Selvarajah

Saim

Idrus

et al. 2020

IJMS

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Nasal mucosa injury can be caused by trauma, radiotherapy, chronic infection such as sinusitis, and post sinus surgery. The rate of healing and its treatment are important in the recovery of patients especially in post sinus surgery, which introduces new injuries. In this review, the current knowledge in terms of the mechanism underlying nasal wound healing was initially discussed. The currently available treatment options for enhancement of wound healing following sinus surgery were discussed and these had included intravenous antibiotics or steroids, various nasal sprays, and nasal packing. In addition, emerging alternative therapies in nasal mucosa wound healing such as herbal medicine and the advancement of regenerative medicine therapies such as stem cells and their byproducts were also discussed. Despite the various available treatment options for wound healing in nasal mucosa, rigorous strong evidence of their efficacy is gravely warranted in order to recommend them as part of the treatment modality.

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Section: Tissue Scaffoldsmentioning

confidence: 99%

Current and Alternative Therapies for Nasal Mucosa Injury: A Review

Selvarajah

Saim

Idrus

et al. 2020

IJMS

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“…More sophisticated models based on tissue engineering are being developed, although they are not applied to the CF field yet. Presently, these models are based on scaffold materials, cell sheet technology, or microfluidic-based devices (lung-on-a-chip) that provide the adhesion, proliferation, and migratory support to the AECs and recapitulate tissue- and organ-level physiology [158]. For example, Blume et al [159] fabricated a multichamber cultured device that integrates standard permeable filter supports containing fully differentiated bronchial epithelial cells which are perfused by basal media simulating the circulation of fluids in the tissue.…”

Section: Future Directions In Tissue Engineeringmentioning

confidence: 99%

Human Cellular Models for the Investigation of Lung Inflammation and Mucus Production in Cystic Fibrosis

Castellani

Gioia

Toma

et al. 2018

Analytical Cellular Pathology

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Chronic inflammation, oxidative stress, mucus plugging, airway remodeling, and respiratory infections are the hallmarks of the cystic fibrosis (CF) lung disease. The airway epithelium is central in the innate immune responses to pathogens colonizing the airways, since it is involved in mucociliary clearance, senses the presence of pathogens, elicits an inflammatory response, orchestrates adaptive immunity, and activates mesenchymal cells. In this review, we focus on cellular models of the human CF airway epithelium that have been used for studying mucus production, inflammatory response, and airway remodeling, with particular reference to two- and three-dimensional cultures that better recapitulate the native airway epithelium. Cocultures of airway epithelial cells, macrophages, dendritic cells, and fibroblasts are instrumental in disease modeling, drug discovery, and identification of novel therapeutic targets. Nevertheless, they have to be implemented in the CF field yet. Finally, novel systems hijacking on tissue engineering, including three-dimensional cocultures, decellularized lungs, microfluidic devices, and lung organoids formed in bioreactors, will lead the generation of relevant human preclinical respiratory models a step forward.

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“…Trapped particles are then cleared by ciliated cells via their ciliary function. Hence, the epithelium protects the underlying connective tissue against the external environment including physical, chemical and microbial factors, and it is also a component of the innate immune system that functions as a protective barrier 1,3 . For these reasons, the lack of a functional airway epithelium in the trachea can lead to severe infections and fibrosis 1 …”

Section: Introductionmentioning

confidence: 99%

“…Hence, the epithelium protects the underlying connective tissue against the external environment including physical, chemical and microbial factors, and it is also a component of the innate immune system that functions as a protective barrier. 1,3 For these reasons, the lack of a functional airway epithelium in the trachea can lead to severe infections and fibrosis. 1 Tracheal disorders include both intrinsic and extrinsic disorders such as stenosis and mediastinal granuloma, respectively.…”

mentioning

confidence: 99%

Regenerating airway epithelium using fibrous biomimetic basement membranes

Gadalla

Tchoukalova

Lott

2022

J Biomedical Materials Res

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There are reciprocal interactions between epithelial cells and underlying basement membrane. The resemblance of biomaterials to native basement membrane is thus critical for their success when used to regenerate epithelium-containing organs. Particularly, the use of nanofibers and the incorporation of basement membrane proteins may mimic both biophysical and biochemical properties of basement membrane, respectively. Herein we tested how electrospun polycaprolactone/ heparin fibers with and without adsorbed laminin and collagen IV proteins affect epithelial cell functions. We found that airway epithelial cells attached, migrated, and proliferated on all scaffolds but protein-functionalized fibers promoted higher attachment, quicker migration, and increased proliferation. Fibers were then integrated on polyethylene scaffolds and cultured at an air-liquid interface. The detection of secretory and ciliated cell markers was higher in cells on polyethylene with fibers. These findings demonstrate that electrospun fibers incite beneficial epithelial cell responses and can be used in the fabrication of bioengineered functional epithelia.

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Prospects and challenges in engineering functional respiratory epithelium for in vitro and in vivo applications

Cited by 7 publications

References 71 publications

Current and Alternative Therapies for Nasal Mucosa Injury: A Review

Current and Alternative Therapies for Nasal Mucosa Injury: A Review

Human Cellular Models for the Investigation of Lung Inflammation and Mucus Production in Cystic Fibrosis

Regenerating airway epithelium using fibrous biomimetic basement membranes

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