“…First of all, it did not include any age-matched healthy controls because it was not designed to compare patients with CdLS with normal children. However, we do not think that this alters the meaningfulness of our results because: (a) the prevalence of otitis media with effusion in healthy infants and children has been widely reported in the literature as 2-30% depending on age and season, with a high rate of spontaneous resolution [Fiellau-Nikolajsen and Loud, 1979;Lous and Fiellau-Nikolajsen, 1981; American Academy of Pediatrics, 2004;Keles et al, 2004b]; (b) its prevalence among healthy Italian children is quite low (up to 20%), consistent over time [Bartolozzi et al, 1992;Marchisio et al, 1998], and periodically checked on a local basis; (c) the family meetings took place in late spring and early autumn, thus making seasonality irrelevant; (d) any allergic environment at the meetings was unlikely to influence the presence of middle ear effusion as most of our patients were not allergic and the findings obtained at the meetings were always confirmed in the hospital setting; and (e) interobserver variability was not a factor because pneumatic otoscopy was always performed by the same investigator (PM).…”