<p class="abstract"><strong>Background:</strong> A prospective study to observe the prevalence of deficiency of vitamin D and calcium in out patients of otolaryngology clinic at R.C.S.M Government medical college, Kolhapur, India. The patients attending outpatient of otolaryngology clinic with various complaints and not responding to conventional treatment were advised for assessment of vitamin D [25 (OH)D] level in blood.</p><p class="abstract"><strong>Methods:</strong> The age, sex, occupation, chief complaints, obesity and provisional diagnosis was noted in all cases. A total of 86 patients were examined, maximum patients were in the age group of 7-15 years. The chief complaints in majority of the patients were sore throat with recurrent upper respiratory tract infection. </p><p class="abstract"><strong>Results:</strong> Only three patient’s vitamin D levels were found to be within normal limits. In rest 83 (96.51%) it was either deficient 57 (66.28%) or insufficient 21 (24.42%).</p><strong>Conclusions:</strong> The incidence of vitamin D deficiency is extremely common in ear, nose and throat disease (ENT) patients. The results of vitamin supplementation were promising in cases of pharyngitis, upper respiratory tract infection (URTI) with asthma, post operation of chronic suppurative otitis media, empirical supplementation of vitamin D in all ENT patients not responding to conventional treatment is worth trying.