Prospective follow-up study of 423 children born after intracytoplasmic sperm injection

Abstract

In order to evaluate the safety of the intracytoplasmic sperm injection (ICSI) procedure, a prospective follow-up study of 423 children born after ICSI was carried out. The aim of this study was to compile data on karyotypes, congenital malformations, growth parameters and developmental milestones. Before starting the infertility treatment, couples were asked to participate in a follow-up study including genetic counselling and prenatal diagnosis. The follow-up study of the child was based on a visit to the paediatrician-geneticist at birth or at 2 months of age, at 1 year and at 2 years of age when a physical examination for major and minor malformations and a psychomotoric evaluation were done. Between April 1991 and September 1994, 320 pregnancies obtained after ICSI led to the birth of 423 children (222 singletons, 186 twins and 15 triplets). Prenatal diagnosis determined a total of 293 karyotypes, one of which was abnormal (0.3%), and four were benign familial structural aberrations, all inherited from the paternal side. A total of 14 (3.3%) major malformations were observed, defined as those causing functional impairment or requiring surgical correlation. Neurological or developmental problems at the age of 2 months were found in 14 children, four of whom were multiples. Compared to most registers of children born after assisted reproduction and to registers of malformations in the general population, the figure of 3.3% major malformations is within the expected range. Before drawing any firm conclusion, further careful evaluations of the available data are necessary.

Genetics of Male Infertility: Evolution of the X and Y Chromosome and Transmission of Male Infertility to Future Generations

“…The incidence of congenital abnormality in ICSI children (2.3%) is no greater than in every normal population studied (Bonduelle et al, 1995(Bonduelle et al, , 1998a(Bonduelle et al, , 1998b(Bonduelle et al, , 1999. Even the few reported ICSI offspring of Klinefelter's patients have been chromosomally normal Staessen et al, 1996;Levron et al, 2000).…”

“…Simple karyotyping of infertile men also raised the possibility of infertility being associated with autosomal translocations (Van Assche et al, 1996; Bonduelle et al, 1995Bonduelle et al, , 1996Bonduelle et al, , 1998aBonduelle et al, , 1998bBonduelle et al, , 1999Egozcue et al, 2000). A massive summary of karyotyping results in newborn populations, reviewed by Van Assche, revealed an incidence of balanced autosomal translocations in a normal newborn population of 0.25% but an incidence of 1.3%, in infertile men (Table Ia) (Van Assche et al., 1996).…”

“…Follow-up of the first 1,987 children born as a result of ICSI has been meticulously studied and reported by the originators of ICSI in the DutchSpeaking Free University of Brussels (Bonduelle et al, 1995(Bonduelle et al, , 1998a(Bonduelle et al, , 1998b(Bonduelle et al, , 1999. In 1,082 karyotypes of ICSI pregnancies, 9 (0.83%) had sex chromosomal abnormalities, including 45,X (Turners), 47, XXY (Klinefelter's), 47, XXX and mosaics of 47, XXX, as well as 47, XYY (Table 6).…”

Safety in Assisted Reproductive Technologies: Insights from Gene Expression Studies During Preimplantation Development

“…The studies were accused of design flows, including the retrospective nature of most of them. In addition, the debate was enlivened by several studies clashing with the evidence of safety risks associated with ART procedures: no evidence for worries in IVF/ICSI babies was in fact repeatedly reported (Bonduelle et al, 1996;Romundstad et al, 2008).…”

Functional morphology of the placental villous tree at term singleton pregnancies, achieved by methods of assisted reproductive technology

“…По данным D. Meschede и др., у них выявляется высокая частота развития врожденных аномалий хромосом и аутосом-ных транслокаций. Возможно, это является наследуемым признаком и передается потом-ству, что повышает у них также риск развития бесплодия [7][8][9]. По данным K.J.…”