Prospection of genetic resistance resources to root-knot nematodes in cucurbit genotypes

Abstract

The aim of this research was to prospect genetic resistant sources to root-knot-nematode in Cucurbitaceae accessions. Two assays were conducted, the first to evaluate the resistance to root-knot nematode, M. incognita race 1 in accessions of Lagenaria vulgaris; Sicana odorifera; Cucurbita facifolia; Luffa sp.; Citrullus lanatus cv. Citroides; Lagenaria vulgaris; Cucurbita moschata; Cucurbita moschata x Cucurbita maxima; Cucumis melo and Cucumis metuliferus. The second to evaluate the reaction to nematode M. incognita race 1, M. javanica and M. enterolobii of the three accessions of Cucumis metuliferus (‘Kino’) considered resistant to M. incognita race 1 in the first experiment. The assays were carried out in a greenhouse at Embrapa Hortaliças in a completely randomized design with six replications. The seedlings in pots were inoculated with 5000 eggs and juveniles of 2nd stage (J2) of each Meloidogyne species alone. Fifty-three and eighty-four days after inoculation, respectively in both experiments, the plants were evaluated for egg mass index (IMO), gall index (IG), number of eggs per gram of root (NEGR) and reproduction factor (RF). The melon Cucumis metuliferus known as ‘Kino’ was identified as genetic source of resistance to root-knot nematodes and can be explored in breeding program as resistant rootstock.

Reactions of Citrullus amarus and Cucumis metuliferus to Meloidogyne chitwoodi, Meloidogyne enterolobii and Meloidogyne luci

“…The main objective of this study was to determine host suitability of C. metuliferus BGV11135 and C. amarus BGV5167 for the nematodes M. chitwoodi, M. enterolobii and M. luci, to provide insights into the potential use of these rootstocks for melon and watermelon crops, and to provide this information to assist management of RKN species. Previous studies have reported resistance of some C. metuliferus accessions to M. incognita, M. arenaria, M. hapla, M. javanica and M. enterolobii (Walters et al, 2006;Ye et al, 2017;Pinheiro et al, 2019), and that of C. amarus to M. arenaria, M. enterolobii, M. incognita and M. javanica (García-Mendívil et al, 2019;Waldo et al, 2023). The present paper is the first report on levels of resistance of C. metuliferus and C. amarus to M. chitwoodi and M. luci.…”

“…In cucurbits, the experimental melon rootstocks Cucumis metuliferus BGV11135 display resistance to M. arenaria, M. incognita and M. javanica (Expósito et al, 2018, and, as well as Citrullus amarus, a commercial watermelon rootstock (García-Mendívil et al, 2019;Waldo et al, 2023). Additionally, three accessions of C. metuliferus 'Kino' exhibit resistance to M. enterolobii, M. incognita race 1, and M. javanica (Pinheiro et al, 2019). Waldo et al (2023) also evaluated 108 different accessions of C. amarus, and some of these were resistant to M. enterolobii.…”

Resistance of Cucurbita spp. germplasm tothe fungus Macrophomina phaseolina

“…Cucurbites in general have morpho-agronomic characteristics that make it possible to use them in a variety of ways, whether in feeding or culture management when used as a rootstock (CHAUDHARI et al, 2017;PINHEIRO et al, 2019;SANTOS et al, 2020;ZHOU et al, 2014).Grafting has been very sought after for cucurbits in the management of diseases, including charcoal rot (COHEN; ELKABETZ; EDELSTEIN, 2016), caused by Macrophomina phaseolina (Tassi) Goid. (1947), which affects several economically important crops of the family, such as melon and watermelon (MEDEIROS et al, 2015).…”

Identification of species of the genus Meloidogyne in cucurbits: Distribution and occurrence in Arequipa, Peru

“…Además, el proceso de infección del sistema radicular contribuye a la entrada de otros fitopatógenos que predisponen a la planta a estrés ambiental perjudicando su desarrollo (Marino et al, 2012;Andrade et al, 2016), y causante de importantes pérdidas económicas en cultivos de climas tropicales y subtropicales (López-Gómez et al, 2015;Sikora et al, 2018), así como en cultivos conducidos en sistemas de invernadero o bajo protección de temperaturas inclementes (Nyczepir, 2013). Se reconoce que la presencia de Meloidogyne es el mayor factor limitante para la producción de cucurbitáceas en el mundo (Pinheiro et al, 2019;Ito et al, 2014).…”