Proposals for optimization of the international phage typing system for Listeria monocytogenes: combined analysis of phage lytic spectrum and variability of typing results

Mee, N. Marquet-Van Der; Audurier, A

doi:10.1128/aem.61.1.303-309.1995

Cited by 8 publications

(5 citation statements)

References 14 publications

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“…It has been shown that a difference of at least two or three major phage reactions is necessary to distinguished between strains of serotype 1/2 or 4, respectively, because of the poor reproducibility observed with some phages used in the international method for phage typing (McLauchlin et al . 1986; Marquet‐van der Mee and Audurier 1995). However, with these criteria, there is still a small probability that clonally‐related strains may be considered distinguishable (Marquet‐van der Mee and Audurier 1995).…”

Section: Resultsmentioning

confidence: 99%

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Comparison of sludge and clinical isolates of Listeria monocytogenes

Lozniewski

Humbert

Corsaro

et al. 2001

Lett Appl Microbiol

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Section: Resultsmentioning

confidence: 99%

“…1986; Marquet‐van der Mee and Audurier 1995). However, with these criteria, there is still a small probability that clonally‐related strains may be considered distinguishable (Marquet‐van der Mee and Audurier 1995). Therefore, the possibility cannot be excluded that S1–S2 and S4, and S28 and S30, are clonally related.…”

Section: Resultsmentioning

confidence: 99%

Comparison of sludge and clinical isolates of Listeria monocytogenes

Lozniewski

Humbert

Corsaro

et al. 2001

Lett Appl Microbiol

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“…In our study the least active phages were 1807 and 6223 (absence of sensitive strains). The least active phage according to Marquet-van der Mee and Audurier (1995) was also phage 1807. In general, the experimental phages lysed very few strains and gave a high percentage of moderate or weak reactions (Table 4).…”

Section: Phage Evaluationmentioning

confidence: 98%

“…Thus, Audurier et al (1979), using a reduced phage set, observed that studying 645 strains isolated in France, the most active phage in the serogroup 1/2 was 12029, and the most speci®c was 1652. More recently, Marquet-van der Mee and Audurier (1995) tested 4697 L. monocytogenes strains isolated in France and found that the most speci®c phages for the serogroup 1/2 were 10, 1967, 387 and 43. In our case they were the same, as well as phage 4477. Phages 43 and 387 were those associated with most frequency as phage 43 produced lysis on 28 occasions, of which 24 coincided with phage 387 (this latter one produced lytic reactions on 25 occasions).…”

Section: Phage Evaluationmentioning

confidence: 99%

“…This system does, however, have some disadvantages, such as the large differences in the ef®ciency of this technique with regard to the geographical area of the strains tested due to the geographical speci®city of the L. monocytogenes strains. Another drawback is the low proportion of phage-typeable serogroup 1/2 strains (Audurier et al 1977a(Audurier et al , 1977b(Audurier et al , 1979(Audurier et al , 1984Rocourt et al 1985;McLauchlin et al 1986;Audurier and Martin 1989;Marquet-van der Mee and Audurier 1995;Rocourt and Cossart 1997;De Simo Ân and Ferrer 1998;Margolles et al 1998). The typeability may be increased by the use of additional phages (McLauchlin et al 1996;Marquet-van der Mee et al 1997).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the international phage typing set and some experimental phages for typing of Listeria monocytogenes from poultry in Spain

et al. 2002

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Aims: The validity of the international phage set and 13 experimental phages for subtyping Listeria monocytogenes strains isolated from poultry in Spain was investigated. Methods and Results: Ninety-six L. monocytogenes strains (52 from serogroup 1/2 and 44 from serogroup 4) were phage-typed using the international phage set, 10 experimental phages for typing serogroup 1/2 strains (seven isolated in France: 1313, 9425, 1807, 351, 881, 717 and 586-, and three from Denmark: 5775, 12682 and 6223-) and three experimental phages isolated in France for typing serogroup 4 strains (2425 A, 4286 and 197). Percentages of serogroup 1/2, serogroup 4 and total phage-typeable strains were 57á7%, 52á3% and 55á2%, respectively. Important differences in the behaviour of the phages tested were found. The typeability rate, the speci®city index and the percentage of strong reactions were greater in the phages of international set than in the experimental phages. The number of phage typeable strains and the number of phage types (42) were not modi®ed by the use of experimental phages. Conclusions: The phage set used was not effective for typing L. monocytogenes strains from poultry in Spain, because a low typeability rate was found. Signi®cance and Impact of the Study: Our results suggest the importance of the availability of new phages speci®c to a geographical area in order to improve the typeability of the system.

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Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

Hughes²,

2005

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Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. As a result more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h. More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid based sequence amplification (NASBA). These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests are available for sub-species characterization, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as multilocus enzyme electrophoresis, phage typing and serotyping. These phenotypic typing methods are being replaced by molecular tests, which reflect genetic relationships between isolates and are more accurate. These new methods are currently mainly used in research but their considerable potential for routine testing in the future cannot be overlooked.

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Proposals for optimization of the international phage typing system for Listeria monocytogenes: combined analysis of phage lytic spectrum and variability of typing results

Cited by 8 publications

References 14 publications

Comparison of sludge and clinical isolates of Listeria monocytogenes

Comparison of sludge and clinical isolates of Listeria monocytogenes

Evaluation of the international phage typing set and some experimental phages for typing of Listeria monocytogenes from poultry in Spain

Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

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