Comparison of sludge and clinical isolates of Listeria monocytogenes

Abstract

Sludge and human L. monocytogenes may be related but further epidemiological studies are necessary to elucidate this point.

Characterization of isolates ofListeria monocytogenesfrom sludge using pulsed-field gel electrophoresis and virulence assays

“…This agrees with the findings of De Luca et al. (1998), Lozniewski et al. (2001) and Paillard et al.…”

“…2005) and in sludge spread onto agricultural land (Watkins and Sleath 1981; Al‐Ghazali and Al‐Azawi 1986; Schwartzbrod et al. 1989; Lozniewski et al. 2001; Paillard et al.…”

“…Listeria monocytogenes strains from sewage sludge have not been studied extensively, and typing of these environmental strains has often been limited to serotyping (De Luca et al. 1998; Lozniewski et al. 2001; Garrec et al.…”

Listeria monocytogenes σ B Has a Small Core Regulon and a Conserved Role in Virulence but Makes Differential Contributions to Stress Tolerance across a Diverse Collection of Strains

“…Characterization of L. monocytogenes isolates from a variety of hosts and environments by multiple subtyping methods (35,63), including multilocus enzyme electrophoresis (MLEE) (47), has shown that strains comprising the species L. monocytogenes belong to two major divisions designated lineages I and II and at least one additional distinct genetic lineage. The third lineage, lineage III, can be subdivided into lineages IIIA/C and IIIB (lineage IIIB has recently been classified as lineage IV [65]), based on results obtained with a number of molecular subtyping strategies, including pulsed-field gel electrophoresis (PFGE) (20) and virulence gene sequencing (20,68).…”

Environmental Reservoir and Transmission into the Mammalian Host

“…Multilocus enzyme electrophoresis (MLEE) and phage typing provided initial insight into the population genetics and transmission of L. monocytogenes. Molecular subtyping methods, including ribotyping (Wiedmann et al 1996, pulsed-field gel electrophoresis (PFGE) (Lozniewski et al 2001;Vela et al 2001), and, more recently, multilocus sequence-based typing (MLST) (Cai et al 2002;Salcedo et al 2003), have provided recent advances in our understanding of L. monocytogenes ecology and transmission and have been used in many studies on L. monocytogenes ecology reported since 1995. While all three molecular methods provide discriminatory power, PFGE was shown to be more discriminatory for L. monocytogenes than MLST or ribotyping.…”